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Effect of american cranberry (Cysticlean) on Escherichia coli adherence to bladder epithelial cells. In vitro and in vivo study.
Arch Esp Urol 2010 Jul-Aug; 63(6):422-30AE

Abstract

OBJECTIVES

The American cranberry proanthocyanidins (PACs) are the main responsible for its efficacy in urinary tract infections. Their mechanism of action is related to inhibition of Escherichia coli to urothelial cells. Cysticlean contains an extract of American cranberry which provides 118 mg of PACs per dose. The activity of Cysticlean tablets on Escherichia Coli adherence to bladder epithelial cells has been studied in vitro. Moreover, the activity of Cistyclean both in powder for oral suspension and tablets has been compared ex-vivo.

METHODS

The rats received both Cysticlean preparations per orem, and urine from each animal was collected during the following 16 hours and preincubated with E. coli. Subsequently, bacteria were incubated with T24 cells. After 1 hour the number of bacteria adhered per cell was calculated. For the in vitro study, E. Coli preincubated at various concentrations of the products were incubated with T24 cells and the same process previously referred was carried out.

RESULTS

Urine samples from rats taking Cysticlean powder for oral suspension and tablets (118 mg PACs/animal) showed an important inhibition of E. Coli adherence (83% and 52%respectively). The inferior dose of 59 mg PACs/animal also showed marked inhibition of E. Coli adherence (29% after Cysticlean tablets intake and 40% for powder). In vitro, Cysticlean showed inhibition of bacterial adherence in all tested concentrations: 5, 25 and 75 PACs mg/ml, diminishing the number og bacteria adhered to epithelial cells by 25%, 36% and 34% respectively.

CONCLUSIONS

Cysticlean shows a significant inhibition of E. Coli adherence to urothelial cells. Cysticlean powder for suspensión preparation is more effective tha tablets. Cysticlean powder for suspensión is well tolerated, and compliance has been observed. Its use is very recommendable in pediatric urinary tract infection prophylaxis. Due to the variety of products with American cranberry extracts in the market, with different proanthocyanidins declared content, it would be interesting to compare their activity using established pharmacological methods.

Authors+Show Affiliations

Facultad de Farmacia de Universidad de Barcelona, Barcelona, Spain. erisco@ub.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng spa

PubMed ID

20820081

Citation

Risco, Ester, et al. "Effect of American Cranberry (Cysticlean) On Escherichia Coli Adherence to Bladder Epithelial Cells. in Vitro and in Vivo Study." Archivos Espanoles De Urologia, vol. 63, no. 6, 2010, pp. 422-30.
Risco E, Miguélez C, Sanchez de Badajoz E, et al. Effect of american cranberry (Cysticlean) on Escherichia coli adherence to bladder epithelial cells. In vitro and in vivo study. Arch Esp Urol. 2010;63(6):422-30.
Risco, E., Miguélez, C., Sanchez de Badajoz, E., & Rouseaud, A. (2010). Effect of american cranberry (Cysticlean) on Escherichia coli adherence to bladder epithelial cells. In vitro and in vivo study. Archivos Espanoles De Urologia, 63(6), pp. 422-30.
Risco E, et al. Effect of American Cranberry (Cysticlean) On Escherichia Coli Adherence to Bladder Epithelial Cells. in Vitro and in Vivo Study. Arch Esp Urol. 2010;63(6):422-30. PubMed PMID: 20820081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of american cranberry (Cysticlean) on Escherichia coli adherence to bladder epithelial cells. In vitro and in vivo study. AU - Risco,Ester, AU - Miguélez,Carlos, AU - Sanchez de Badajoz,Eduardo, AU - Rouseaud,Alberto, PY - 2010/9/8/entrez PY - 2010/9/8/pubmed PY - 2011/1/12/medline SP - 422 EP - 30 JF - Archivos espanoles de urologia JO - Arch. Esp. Urol. VL - 63 IS - 6 N2 - OBJECTIVES: The American cranberry proanthocyanidins (PACs) are the main responsible for its efficacy in urinary tract infections. Their mechanism of action is related to inhibition of Escherichia coli to urothelial cells. Cysticlean contains an extract of American cranberry which provides 118 mg of PACs per dose. The activity of Cysticlean tablets on Escherichia Coli adherence to bladder epithelial cells has been studied in vitro. Moreover, the activity of Cistyclean both in powder for oral suspension and tablets has been compared ex-vivo. METHODS: The rats received both Cysticlean preparations per orem, and urine from each animal was collected during the following 16 hours and preincubated with E. coli. Subsequently, bacteria were incubated with T24 cells. After 1 hour the number of bacteria adhered per cell was calculated. For the in vitro study, E. Coli preincubated at various concentrations of the products were incubated with T24 cells and the same process previously referred was carried out. RESULTS: Urine samples from rats taking Cysticlean powder for oral suspension and tablets (118 mg PACs/animal) showed an important inhibition of E. Coli adherence (83% and 52%respectively). The inferior dose of 59 mg PACs/animal also showed marked inhibition of E. Coli adherence (29% after Cysticlean tablets intake and 40% for powder). In vitro, Cysticlean showed inhibition of bacterial adherence in all tested concentrations: 5, 25 and 75 PACs mg/ml, diminishing the number og bacteria adhered to epithelial cells by 25%, 36% and 34% respectively. CONCLUSIONS: Cysticlean shows a significant inhibition of E. Coli adherence to urothelial cells. Cysticlean powder for suspensión preparation is more effective tha tablets. Cysticlean powder for suspensión is well tolerated, and compliance has been observed. Its use is very recommendable in pediatric urinary tract infection prophylaxis. Due to the variety of products with American cranberry extracts in the market, with different proanthocyanidins declared content, it would be interesting to compare their activity using established pharmacological methods. SN - 1576-8260 UR - https://www.unboundmedicine.com/medline/citation/20820081/Effect_of_american_cranberry__Cysticlean__on_Escherichia_coli_adherence_to_bladder_epithelial_cells__In_vitro_and_in_vivo_study_ L2 - http://www.aeurologia.com/articulo_prod.php?id_art=7070914951785 DB - PRIME DP - Unbound Medicine ER -