Tags

Type your tag names separated by a space and hit enter

[Effects of Shaoqiduogan on MMP-13, TIMP-1 expression in liver and hepatic stellate cells of hepatic fibrosis rats].
Zhongguo Zhong Yao Za Zhi 2010; 35(11):1447-51ZZ

Abstract

OBJECTIVE

To investigate the effects of Shaoqiduogan (SQDG) on the expression of matrix metalloproteinase 13 (MMP-13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in carbon tetrachloride (CCl4) induced hepatic fibrosis rats and transforming growth factor beta1 (TGF-beta1) irritated hepatic stellate cells (HSC), and to explore its possible mechanisms.

METHOD

The model of chemical hepatic fibrosis induced by CCl4 was prepared. The rats were randomly divided into 5 groups, including normal control group, liver fibrosis model group and SQDG (42. 5, 85, 170 mg x kg(-1)) treated groups. The level of collagen type 1 (C-1) in serum was determined by radioimmunoassay. Masson stain was used to examine the histopathological change. MMP-13 and TIMP-1 ex-pression in liver tissues were assayed by immunohistochemistry. In vitro, effects of SQDG on the expression of MMP-13, TIMP-1 and C-1 in HSC-T6 stimulated by TGF-beta were measured by Western-blot.

RESULT

The results showed that SQDG significantly decreased the elevated level of C-1 in serum of hepatic fibrosis rats induced by CCl4. Pathological examination showed that SQDG could remarkably alleviate the degree of liver fibrogenesis and formation of pseudolobulus. The results of immunohistochemistry demonstrated that SQDG significantly increased MMP-13 expression and decreased TIMP-1 expression in liver tissues. Furthermore, SQDG (20-160 mg x L(-1)) could facilitate MMP-13 expression, inhibit TIMP-1 expression and significantly inhibit the C-I production of HSC stimulated with TGF-beta1 in vitro.

CONCLUSION

The anti-fibrotic effects of SQDG may be associated with its action of promoting collagen degradation via controlling the levels of MMP-13 and TIMP-1 in liver.

Authors+Show Affiliations

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immunopharmacology of Education Ministry, Anhui Engineering Technology Research Center of Anti-inflammatory and Immunodrugs, Hefei 230032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

20822018

Citation

Sun, Wuyi, et al. "[Effects of Shaoqiduogan On MMP-13, TIMP-1 Expression in Liver and Hepatic Stellate Cells of Hepatic Fibrosis Rats]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 35, no. 11, 2010, pp. 1447-51.
Sun W, Gui S, Wu L, et al. [Effects of Shaoqiduogan on MMP-13, TIMP-1 expression in liver and hepatic stellate cells of hepatic fibrosis rats]. Zhongguo Zhong Yao Za Zhi. 2010;35(11):1447-51.
Sun, W., Gui, S., Wu, L., Wang, H., & Wei, W. (2010). [Effects of Shaoqiduogan on MMP-13, TIMP-1 expression in liver and hepatic stellate cells of hepatic fibrosis rats]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 35(11), pp. 1447-51.
Sun W, et al. [Effects of Shaoqiduogan On MMP-13, TIMP-1 Expression in Liver and Hepatic Stellate Cells of Hepatic Fibrosis Rats]. Zhongguo Zhong Yao Za Zhi. 2010;35(11):1447-51. PubMed PMID: 20822018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Shaoqiduogan on MMP-13, TIMP-1 expression in liver and hepatic stellate cells of hepatic fibrosis rats]. AU - Sun,Wuyi, AU - Gui,Shuangying, AU - Wu,Li, AU - Wang,Hua, AU - Wei,Wei, PY - 2010/9/9/entrez PY - 2010/9/9/pubmed PY - 2010/10/6/medline SP - 1447 EP - 51 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 35 IS - 11 N2 - OBJECTIVE: To investigate the effects of Shaoqiduogan (SQDG) on the expression of matrix metalloproteinase 13 (MMP-13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in carbon tetrachloride (CCl4) induced hepatic fibrosis rats and transforming growth factor beta1 (TGF-beta1) irritated hepatic stellate cells (HSC), and to explore its possible mechanisms. METHOD: The model of chemical hepatic fibrosis induced by CCl4 was prepared. The rats were randomly divided into 5 groups, including normal control group, liver fibrosis model group and SQDG (42. 5, 85, 170 mg x kg(-1)) treated groups. The level of collagen type 1 (C-1) in serum was determined by radioimmunoassay. Masson stain was used to examine the histopathological change. MMP-13 and TIMP-1 ex-pression in liver tissues were assayed by immunohistochemistry. In vitro, effects of SQDG on the expression of MMP-13, TIMP-1 and C-1 in HSC-T6 stimulated by TGF-beta were measured by Western-blot. RESULT: The results showed that SQDG significantly decreased the elevated level of C-1 in serum of hepatic fibrosis rats induced by CCl4. Pathological examination showed that SQDG could remarkably alleviate the degree of liver fibrogenesis and formation of pseudolobulus. The results of immunohistochemistry demonstrated that SQDG significantly increased MMP-13 expression and decreased TIMP-1 expression in liver tissues. Furthermore, SQDG (20-160 mg x L(-1)) could facilitate MMP-13 expression, inhibit TIMP-1 expression and significantly inhibit the C-I production of HSC stimulated with TGF-beta1 in vitro. CONCLUSION: The anti-fibrotic effects of SQDG may be associated with its action of promoting collagen degradation via controlling the levels of MMP-13 and TIMP-1 in liver. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/20822018/[Effects_of_Shaoqiduogan_on_MMP_13_TIMP_1_expression_in_liver_and_hepatic_stellate_cells_of_hepatic_fibrosis_rats]_ L2 - http://www.diseaseinfosearch.org/result/3329 DB - PRIME DP - Unbound Medicine ER -