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Gene limiting cadmium accumulation in rice.
Proc Natl Acad Sci U S A. 2010 Sep 21; 107(38):16500-5.PN

Abstract

Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P(1B)-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

Authors+Show Affiliations

Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20823253

Citation

Ueno, Daisei, et al. "Gene Limiting Cadmium Accumulation in Rice." Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 38, 2010, pp. 16500-5.
Ueno D, Yamaji N, Kono I, et al. Gene limiting cadmium accumulation in rice. Proc Natl Acad Sci U S A. 2010;107(38):16500-5.
Ueno, D., Yamaji, N., Kono, I., Huang, C. F., Ando, T., Yano, M., & Ma, J. F. (2010). Gene limiting cadmium accumulation in rice. Proceedings of the National Academy of Sciences of the United States of America, 107(38), 16500-5. https://doi.org/10.1073/pnas.1005396107
Ueno D, et al. Gene Limiting Cadmium Accumulation in Rice. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16500-5. PubMed PMID: 20823253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene limiting cadmium accumulation in rice. AU - Ueno,Daisei, AU - Yamaji,Naoki, AU - Kono,Izumi, AU - Huang,Chao Feng, AU - Ando,Tsuyu, AU - Yano,Masahiro, AU - Ma,Jian Feng, Y1 - 2010/09/07/ PY - 2010/9/9/entrez PY - 2010/9/9/pubmed PY - 2010/11/3/medline SP - 16500 EP - 5 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 107 IS - 38 N2 - Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P(1B)-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/20823253/Gene_limiting_cadmium_accumulation_in_rice_ DB - PRIME DP - Unbound Medicine ER -