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Rapid determination of caffeoylquinic acid derivatives in Cynara scolymus L. by ultra-fast liquid chromatography/tandem mass spectrometry based on a fused core C18 column.
J Sep Sci. 2010 Oct; 33(20):3152-8.JS

Abstract

An ultra-fast high-performance LC-ESI-MS/MS method was developed for the analysis and quantification of caffeoylquinic acid derivatives, including chlorogenic acid, 1,3-di-O-caffeoylquinic acid (cynarin) and 1,5-di-O-caffeoylquinic acid, in artichoke (Cynara scolymus L.) heads and leaves. The rapid separation (less than 4 min) was achieved based on a Halo fused core C18-silica column (50 mm × 2.1 mm id, 2.7 μm). The target compounds were detected and quantified by a triple-quadrupole mass spectrometer in multiple-reaction monitoring mode. The calibration function is linear from 0.06 to 2800 ng/mL for chlorogenic acid, 0.3-3000 ng/mL for cynarin and 0.24-4800 ng/mL for 1,5-di-O-caffeoylquinic acid, respectively. The average recoveries ranged from 92.1 to 113.2% with RSDs ≤6.5%. Moreover, four batches of artichoke head and leaf extracts were analyzed using the established method. The results indicated that the Halo fused core column provided much faster separations and higher sample throughput without sacrificing column ruggedness and reliability, and triple-quadrupole MS provided extraordinarily lower LOQs for most of the target analytes. Comparing to conventional quantitative approaches, the established method was fast, sensitive and reliable for the determination of caffeoylquinic acid derivatives in artichoke.

Authors+Show Affiliations

College of Food Science and Biological Engineering, Zhejiang Gongshang University, Hangzhou, PR China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

20824661

Citation

Shen, Qing, et al. "Rapid Determination of Caffeoylquinic Acid Derivatives in Cynara Scolymus L. By Ultra-fast Liquid Chromatography/tandem Mass Spectrometry Based On a Fused Core C18 Column." Journal of Separation Science, vol. 33, no. 20, 2010, pp. 3152-8.
Shen Q, Dai Z, Lu Y. Rapid determination of caffeoylquinic acid derivatives in Cynara scolymus L. by ultra-fast liquid chromatography/tandem mass spectrometry based on a fused core C18 column. J Sep Sci. 2010;33(20):3152-8.
Shen, Q., Dai, Z., & Lu, Y. (2010). Rapid determination of caffeoylquinic acid derivatives in Cynara scolymus L. by ultra-fast liquid chromatography/tandem mass spectrometry based on a fused core C18 column. Journal of Separation Science, 33(20), 3152-8. https://doi.org/10.1002/jssc.201000397
Shen Q, Dai Z, Lu Y. Rapid Determination of Caffeoylquinic Acid Derivatives in Cynara Scolymus L. By Ultra-fast Liquid Chromatography/tandem Mass Spectrometry Based On a Fused Core C18 Column. J Sep Sci. 2010;33(20):3152-8. PubMed PMID: 20824661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid determination of caffeoylquinic acid derivatives in Cynara scolymus L. by ultra-fast liquid chromatography/tandem mass spectrometry based on a fused core C18 column. AU - Shen,Qing, AU - Dai,Zhiyuan, AU - Lu,Yanbin, PY - 2010/9/9/entrez PY - 2010/9/9/pubmed PY - 2011/3/1/medline SP - 3152 EP - 8 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 20 N2 - An ultra-fast high-performance LC-ESI-MS/MS method was developed for the analysis and quantification of caffeoylquinic acid derivatives, including chlorogenic acid, 1,3-di-O-caffeoylquinic acid (cynarin) and 1,5-di-O-caffeoylquinic acid, in artichoke (Cynara scolymus L.) heads and leaves. The rapid separation (less than 4 min) was achieved based on a Halo fused core C18-silica column (50 mm × 2.1 mm id, 2.7 μm). The target compounds were detected and quantified by a triple-quadrupole mass spectrometer in multiple-reaction monitoring mode. The calibration function is linear from 0.06 to 2800 ng/mL for chlorogenic acid, 0.3-3000 ng/mL for cynarin and 0.24-4800 ng/mL for 1,5-di-O-caffeoylquinic acid, respectively. The average recoveries ranged from 92.1 to 113.2% with RSDs ≤6.5%. Moreover, four batches of artichoke head and leaf extracts were analyzed using the established method. The results indicated that the Halo fused core column provided much faster separations and higher sample throughput without sacrificing column ruggedness and reliability, and triple-quadrupole MS provided extraordinarily lower LOQs for most of the target analytes. Comparing to conventional quantitative approaches, the established method was fast, sensitive and reliable for the determination of caffeoylquinic acid derivatives in artichoke. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20824661/Rapid_determination_of_caffeoylquinic_acid_derivatives_in_Cynara_scolymus_L__by_ultra_fast_liquid_chromatography/tandem_mass_spectrometry_based_on_a_fused_core_C18_column_ L2 - https://doi.org/10.1002/jssc.201000397 DB - PRIME DP - Unbound Medicine ER -