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Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16.
Biochem Biophys Res Commun 2010; 400(4):613-8BB

Abstract

Dimethyl sulfoxide (DMSO) is an important solvent that is widely used in industry and medical studies, as well as in the study of aging, in which it is used as a negative control for lifespan assays; however, our data showed that 0.5% and 2% DMSO extended the lifespan of Caenorhabditis elegans by 24.4% and 23.0% (the first trial), respectively. Treatment with 0.5% DMSO did not affect the progeny number or the lifespan of C. elegans under thermal stress. Using real time reverse transcription-polymerase chain reaction (RT-PCR), we found that the expression levels of hsp-16.2, hsp-70, lys-7, old-1, and sod-5 were enhanced by 2.5, 2.9, 1.3, 2.3, and 4.5-fold, respectively, after treatment with 0.5% DMSO. This suggests that these genes downstream of DAF-16 might function in the lifespan extension properties of DMSO. Using the transgenic strain lys-7::GFP, we found that treatment with 0.5% DMSO also caused expression levels of lys-7 increased by 1.5-fold. Genetic analysis using mutants of aging-related genes showed that lifespan extension in C. elegans by DMSO was dependent on sir-2.1 and daf-16 but not eat-2 or hsf-1. In summary, we report the function and the putative mechanism of DMSO in lifespan extension of C. elegans. This study draws attention to using DMSO as a solvent when conducting aging studies.

Authors+Show Affiliations

The Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China. xm224@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20828537

Citation

Wang, Xiangming, et al. "Lifespan Extension in Caenorhabditis Elegans By DMSO Is Dependent On Sir-2.1 and Daf-16." Biochemical and Biophysical Research Communications, vol. 400, no. 4, 2010, pp. 613-8.
Wang X, Wang X, Li L, et al. Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16. Biochem Biophys Res Commun. 2010;400(4):613-8.
Wang, X., Wang, X., Li, L., & Wang, D. (2010). Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16. Biochemical and Biophysical Research Communications, 400(4), pp. 613-8. doi:10.1016/j.bbrc.2010.08.113.
Wang X, et al. Lifespan Extension in Caenorhabditis Elegans By DMSO Is Dependent On Sir-2.1 and Daf-16. Biochem Biophys Res Commun. 2010 Oct 1;400(4):613-8. PubMed PMID: 20828537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16. AU - Wang,Xiangming, AU - Wang,Xiaoyan, AU - Li,Lianda, AU - Wang,Danqiao, Y1 - 2010/09/07/ PY - 2010/08/13/received PY - 2010/08/25/accepted PY - 2010/9/11/entrez PY - 2010/9/11/pubmed PY - 2010/12/14/medline SP - 613 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 400 IS - 4 N2 - Dimethyl sulfoxide (DMSO) is an important solvent that is widely used in industry and medical studies, as well as in the study of aging, in which it is used as a negative control for lifespan assays; however, our data showed that 0.5% and 2% DMSO extended the lifespan of Caenorhabditis elegans by 24.4% and 23.0% (the first trial), respectively. Treatment with 0.5% DMSO did not affect the progeny number or the lifespan of C. elegans under thermal stress. Using real time reverse transcription-polymerase chain reaction (RT-PCR), we found that the expression levels of hsp-16.2, hsp-70, lys-7, old-1, and sod-5 were enhanced by 2.5, 2.9, 1.3, 2.3, and 4.5-fold, respectively, after treatment with 0.5% DMSO. This suggests that these genes downstream of DAF-16 might function in the lifespan extension properties of DMSO. Using the transgenic strain lys-7::GFP, we found that treatment with 0.5% DMSO also caused expression levels of lys-7 increased by 1.5-fold. Genetic analysis using mutants of aging-related genes showed that lifespan extension in C. elegans by DMSO was dependent on sir-2.1 and daf-16 but not eat-2 or hsf-1. In summary, we report the function and the putative mechanism of DMSO in lifespan extension of C. elegans. This study draws attention to using DMSO as a solvent when conducting aging studies. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/20828537/Lifespan_extension_in_Caenorhabditis_elegans_by_DMSO_is_dependent_on_sir_2_1_and_daf_16_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(10)01634-7 DB - PRIME DP - Unbound Medicine ER -