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Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring.
Ecotoxicol Environ Saf. 2011 Jan; 74(1):144-9.EE

Abstract

This study concerns a comparative analysis of the acridine orange and Giemsa staining procedures for the fish erythrocyte micronucleus assay. The goal was to optimize the assay in the context of field water monitoring. Fish (Carassius carassius) were exposed to a reference genotoxic agent, cyclophosphamide monohydrate 5 mg l(-1) for 2, 4, and 6 days before testing. Slides from each individual were scored using the two procedures. The results show that the assay was more sensitive when acridine orange was used. When slides were Giemsa stained, the presence of ambiguous artefacts, leading to false positives and increasing random variance, reduced the contrast between exposed and control samples. Acridine Orange staining was then applied in the context of water quality monitoring. Fish were exposed for 4 days to water sampled in two hydrological contexts: basal flow and spring flood. The results show that exposure to spring flood water in an agricultural stream can induce mutagenicity.

Authors+Show Affiliations

Université de Toulouse; INP-ENSAT, Avenue de l'Agrobiopôle, 31326 Castanet-Tolosan, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20828819

Citation

Polard, T, et al. "Giemsa Versus Acridine Orange Staining in the Fish Micronucleus Assay and Validation for Use in Water Quality Monitoring." Ecotoxicology and Environmental Safety, vol. 74, no. 1, 2011, pp. 144-9.
Polard T, Jean S, Merlina G, et al. Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring. Ecotoxicol Environ Saf. 2011;74(1):144-9.
Polard, T., Jean, S., Merlina, G., Laplanche, C., Pinelli, E., & Gauthier, L. (2011). Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring. Ecotoxicology and Environmental Safety, 74(1), 144-9. https://doi.org/10.1016/j.ecoenv.2010.08.005
Polard T, et al. Giemsa Versus Acridine Orange Staining in the Fish Micronucleus Assay and Validation for Use in Water Quality Monitoring. Ecotoxicol Environ Saf. 2011;74(1):144-9. PubMed PMID: 20828819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring. AU - Polard,T, AU - Jean,S, AU - Merlina,G, AU - Laplanche,C, AU - Pinelli,E, AU - Gauthier,L, Y1 - 2010/09/15/ PY - 2009/11/26/received PY - 2010/07/29/revised PY - 2010/08/01/accepted PY - 2010/9/11/entrez PY - 2010/9/11/pubmed PY - 2011/2/15/medline SP - 144 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 74 IS - 1 N2 - This study concerns a comparative analysis of the acridine orange and Giemsa staining procedures for the fish erythrocyte micronucleus assay. The goal was to optimize the assay in the context of field water monitoring. Fish (Carassius carassius) were exposed to a reference genotoxic agent, cyclophosphamide monohydrate 5 mg l(-1) for 2, 4, and 6 days before testing. Slides from each individual were scored using the two procedures. The results show that the assay was more sensitive when acridine orange was used. When slides were Giemsa stained, the presence of ambiguous artefacts, leading to false positives and increasing random variance, reduced the contrast between exposed and control samples. Acridine Orange staining was then applied in the context of water quality monitoring. Fish were exposed for 4 days to water sampled in two hydrological contexts: basal flow and spring flood. The results show that exposure to spring flood water in an agricultural stream can induce mutagenicity. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/20828819/Giemsa_versus_acridine_orange_staining_in_the_fish_micronucleus_assay_and_validation_for_use_in_water_quality_monitoring_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(10)00202-2 DB - PRIME DP - Unbound Medicine ER -