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Electrochemical determination of guanine and adenine by CdS microspheres modified electrode and evaluation of damage to DNA purine bases by UV radiation.
Biosens Bioelectron. 2010 Oct 15; 26(2):314-20.BB

Abstract

Sensitive electrochemical sensor based on a composite of novel pearl-like CdS microspheres and chitosan (CdS-CHIT/GCE) was constructed and characterized using cyclic voltammetry. Single-stranded (ssDNA) and double-stranded (dsDNA) deoxyribonuleic acid were electrochemically adsorbed onto CdS-CHIT/GCE and a significant difference in electrochemical impedance spectra of the ssDNA/CdS-CHIT/GCE and dsDNA/CdS-CHIT/GCE electrodes was found. Electrocatalytic properties of CdS allowed to use the CdS-CHIT/GCE successfully for the trace determination of simple guanine and adenine with nanomolar detection limits by differential pulse voltammetry (DPV). Moreover, damage to the DNA purine bases, guanine and adenine, liberated in previously hydrolyzed calf thymus dsDNA, caused by UV-B, UV-C, and visible light was evaluated. While only minor changes in anodic DPV response of guanine and adenine could be seen after 60 min exposition to UV-B and visible light, total degradation of DNA bases was observed after 20 min exposure to UV-C. A great potential of the CdS microspheres used as the interface at the nucleic acid based biosensors was demonstrated.

Authors+Show Affiliations

University of Eastern Finland, Laboratory of Applied Environmental Chemistry, Patteristonkatu 1, FI-50100 Mikkeli, Finland. Adriana.Ferancova@uef.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20829020

Citation

Ferancová, A, et al. "Electrochemical Determination of Guanine and Adenine By CdS Microspheres Modified Electrode and Evaluation of Damage to DNA Purine Bases By UV Radiation." Biosensors & Bioelectronics, vol. 26, no. 2, 2010, pp. 314-20.
Ferancová A, Rengaraj S, Kim Y, et al. Electrochemical determination of guanine and adenine by CdS microspheres modified electrode and evaluation of damage to DNA purine bases by UV radiation. Biosens Bioelectron. 2010;26(2):314-20.
Ferancová, A., Rengaraj, S., Kim, Y., Labuda, J., & Sillanpää, M. (2010). Electrochemical determination of guanine and adenine by CdS microspheres modified electrode and evaluation of damage to DNA purine bases by UV radiation. Biosensors & Bioelectronics, 26(2), 314-20. https://doi.org/10.1016/j.bios.2010.08.026
Ferancová A, et al. Electrochemical Determination of Guanine and Adenine By CdS Microspheres Modified Electrode and Evaluation of Damage to DNA Purine Bases By UV Radiation. Biosens Bioelectron. 2010 Oct 15;26(2):314-20. PubMed PMID: 20829020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical determination of guanine and adenine by CdS microspheres modified electrode and evaluation of damage to DNA purine bases by UV radiation. AU - Ferancová,A, AU - Rengaraj,S, AU - Kim,Y, AU - Labuda,J, AU - Sillanpää,M, Y1 - 2010/08/19/ PY - 2010/04/21/received PY - 2010/08/04/revised PY - 2010/08/12/accepted PY - 2010/9/11/entrez PY - 2010/9/11/pubmed PY - 2011/2/8/medline SP - 314 EP - 20 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 2 N2 - Sensitive electrochemical sensor based on a composite of novel pearl-like CdS microspheres and chitosan (CdS-CHIT/GCE) was constructed and characterized using cyclic voltammetry. Single-stranded (ssDNA) and double-stranded (dsDNA) deoxyribonuleic acid were electrochemically adsorbed onto CdS-CHIT/GCE and a significant difference in electrochemical impedance spectra of the ssDNA/CdS-CHIT/GCE and dsDNA/CdS-CHIT/GCE electrodes was found. Electrocatalytic properties of CdS allowed to use the CdS-CHIT/GCE successfully for the trace determination of simple guanine and adenine with nanomolar detection limits by differential pulse voltammetry (DPV). Moreover, damage to the DNA purine bases, guanine and adenine, liberated in previously hydrolyzed calf thymus dsDNA, caused by UV-B, UV-C, and visible light was evaluated. While only minor changes in anodic DPV response of guanine and adenine could be seen after 60 min exposition to UV-B and visible light, total degradation of DNA bases was observed after 20 min exposure to UV-C. A great potential of the CdS microspheres used as the interface at the nucleic acid based biosensors was demonstrated. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20829020/Electrochemical_determination_of_guanine_and_adenine_by_CdS_microspheres_modified_electrode_and_evaluation_of_damage_to_DNA_purine_bases_by_UV_radiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00530-0 DB - PRIME DP - Unbound Medicine ER -