Tags

Type your tag names separated by a space and hit enter

Gymnasterkoreayne F inhibits osteoclast formation by suppressing NFATc1 and DC-STAMP expression.
Int Immunopharmacol. 2010 Nov; 10(11):1440-7.II

Abstract

Osteoclasts are multinucleated cells that have a unique role in bone degradation. Modulation of osteoclast formation and/or its activity is an important approach for the treatment of bone-destructive diseases such as osteoporosis and rheumatoid arthritis. In this study, Gymnasterkoreayne F (GK-F), a natural compound isolated from Gymnaster koraiensis, was found to inhibit osteoclast differentiation from primary bone marrow-derived macrophages (BMMs) in a dose-dependent manner. The inhibition occurred through the suppression of nuclear factor of activated T cells c1 (NFATc1) expression, which then led to the decreased levels of osteoclastogenic markers, including Cathepsin K and tartrate-resistant acid phosphatase (TRAP). In addition, GK-F abolished pre-osteoclast fusion induced by the receptor activator of nuclear factor kappa B ligand (RANKL), lipopolysaccharide (LPS) and TNF-α. Reflecting its inhibitory effects on cell-cell fusion, GK-F attenuated the gene expression of an essential molecule of osteoclast fusion, the dendritic cell-specific transmembrane protein (DC-STAMP). Furthermore, GK-F inhibited the bone resorptive activity of differentiated osteoclasts through its ability to block RANKL-induced actin ring formation. The effect was associated with a significant decrease in the induction of β3 integrin expression, which is an essential regulator of osteoclast cytoskeletal function. Taken together, these results suggest that GK-F might be useful as a therapeutic agent for bone resorption-related diseases.

Authors+Show Affiliations

Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Jung-gu, Daegu, Republic of Korea. biohjk@knu.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20831919

Citation

Kim, Hyun-Ju, et al. "Gymnasterkoreayne F Inhibits Osteoclast Formation By Suppressing NFATc1 and DC-STAMP Expression." International Immunopharmacology, vol. 10, no. 11, 2010, pp. 1440-7.
Kim HJ, Hong J, Jung JW, et al. Gymnasterkoreayne F inhibits osteoclast formation by suppressing NFATc1 and DC-STAMP expression. Int Immunopharmacol. 2010;10(11):1440-7.
Kim, H. J., Hong, J., Jung, J. W., Kim, T. H., Kim, J. A., Kim, Y. H., & Kim, S. Y. (2010). Gymnasterkoreayne F inhibits osteoclast formation by suppressing NFATc1 and DC-STAMP expression. International Immunopharmacology, 10(11), 1440-7. https://doi.org/10.1016/j.intimp.2010.08.017
Kim HJ, et al. Gymnasterkoreayne F Inhibits Osteoclast Formation By Suppressing NFATc1 and DC-STAMP Expression. Int Immunopharmacol. 2010;10(11):1440-7. PubMed PMID: 20831919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gymnasterkoreayne F inhibits osteoclast formation by suppressing NFATc1 and DC-STAMP expression. AU - Kim,Hyun-Ju, AU - Hong,JungMin, AU - Jung,Ji-Won, AU - Kim,Tae-Ho, AU - Kim,Jeong Ah, AU - Kim,Young Ho, AU - Kim,Shin-Yoon, Y1 - 2010/09/08/ PY - 2010/03/30/received PY - 2010/08/06/revised PY - 2010/08/20/accepted PY - 2010/9/14/entrez PY - 2010/9/14/pubmed PY - 2011/3/4/medline SP - 1440 EP - 7 JF - International immunopharmacology JO - Int Immunopharmacol VL - 10 IS - 11 N2 - Osteoclasts are multinucleated cells that have a unique role in bone degradation. Modulation of osteoclast formation and/or its activity is an important approach for the treatment of bone-destructive diseases such as osteoporosis and rheumatoid arthritis. In this study, Gymnasterkoreayne F (GK-F), a natural compound isolated from Gymnaster koraiensis, was found to inhibit osteoclast differentiation from primary bone marrow-derived macrophages (BMMs) in a dose-dependent manner. The inhibition occurred through the suppression of nuclear factor of activated T cells c1 (NFATc1) expression, which then led to the decreased levels of osteoclastogenic markers, including Cathepsin K and tartrate-resistant acid phosphatase (TRAP). In addition, GK-F abolished pre-osteoclast fusion induced by the receptor activator of nuclear factor kappa B ligand (RANKL), lipopolysaccharide (LPS) and TNF-α. Reflecting its inhibitory effects on cell-cell fusion, GK-F attenuated the gene expression of an essential molecule of osteoclast fusion, the dendritic cell-specific transmembrane protein (DC-STAMP). Furthermore, GK-F inhibited the bone resorptive activity of differentiated osteoclasts through its ability to block RANKL-induced actin ring formation. The effect was associated with a significant decrease in the induction of β3 integrin expression, which is an essential regulator of osteoclast cytoskeletal function. Taken together, these results suggest that GK-F might be useful as a therapeutic agent for bone resorption-related diseases. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/20831919/Gymnasterkoreayne_F_inhibits_osteoclast_formation_by_suppressing_NFATc1_and_DC_STAMP_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(10)00271-7 DB - PRIME DP - Unbound Medicine ER -