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Characterization of oil bodies in adlay (Coix lachryma-jobi L).
Biosci Biotechnol Biochem. 2010; 74(9):1841-7.BB

Abstract

Oil bodies were observed in cells of both embryo and aleurone layers of mature adlay grains (Coix lachryma-jobi L. var. ma-yuen Stapf). Stable oil bodies were successfully isolated from the adlay grains. Thin-layer chromatography revealed that the contents stored in the adlay oil bodies were mainly neutral lipids (>90% triacylglycerols and about 5% diacylglycerols). The integrity of the isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms (termed oleosin-H and oleosin-L) and one caleosin were present in the adlay oil bodies. Full-length cDNA fragments encoding these three unique oil-body proteins were obtained by PCR cloning. MALDI-MS analyses confirmed that the three full-length cDNA fragments encoded the two oleosin isoforms and one caleosin observed in the oil bodies isolated from the adlay grains.

Authors+Show Affiliations

Department of Food Science, Tunghai University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20834154

Citation

Lu, Hsi-Chi, et al. "Characterization of Oil Bodies in Adlay (Coix Lachryma-jobi L)." Bioscience, Biotechnology, and Biochemistry, vol. 74, no. 9, 2010, pp. 1841-7.
Lu HC, Jiang PL, Hsu LR, et al. Characterization of oil bodies in adlay (Coix lachryma-jobi L). Biosci Biotechnol Biochem. 2010;74(9):1841-7.
Lu, H. C., Jiang, P. L., Hsu, L. R., Chyan, C. L., & Tzen, J. T. (2010). Characterization of oil bodies in adlay (Coix lachryma-jobi L). Bioscience, Biotechnology, and Biochemistry, 74(9), 1841-7.
Lu HC, et al. Characterization of Oil Bodies in Adlay (Coix Lachryma-jobi L). Biosci Biotechnol Biochem. 2010;74(9):1841-7. PubMed PMID: 20834154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of oil bodies in adlay (Coix lachryma-jobi L). AU - Lu,Hsi-Chi, AU - Jiang,Pei-Luen, AU - Hsu,Light R C, AU - Chyan,Chia-Lin, AU - Tzen,Jason T C, Y1 - 2010/09/07/ PY - 2010/9/14/entrez PY - 2010/9/14/pubmed PY - 2011/2/10/medline SP - 1841 EP - 7 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 74 IS - 9 N2 - Oil bodies were observed in cells of both embryo and aleurone layers of mature adlay grains (Coix lachryma-jobi L. var. ma-yuen Stapf). Stable oil bodies were successfully isolated from the adlay grains. Thin-layer chromatography revealed that the contents stored in the adlay oil bodies were mainly neutral lipids (>90% triacylglycerols and about 5% diacylglycerols). The integrity of the isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms (termed oleosin-H and oleosin-L) and one caleosin were present in the adlay oil bodies. Full-length cDNA fragments encoding these three unique oil-body proteins were obtained by PCR cloning. MALDI-MS analyses confirmed that the three full-length cDNA fragments encoded the two oleosin isoforms and one caleosin observed in the oil bodies isolated from the adlay grains. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/20834154/Characterization_of_oil_bodies_in_adlay__Coix_lachryma_jobi_L__ L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/bbb/100247?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -