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Collagen-PCL sheath-core bicomponent electrospun scaffolds increase osteogenic differentiation and calcium accretion of human adipose-derived stem cells.
J Biomater Sci Polym Ed. 2011; 22(13):1695-712.JB

Abstract

Human adipose-derived stem cells (hASCs) are an abundant cell source capable of osteogenic differentiation, and have been investigated as an autologous stem cell source for bone tissue engineering applications. The objective of this study was to determine if the addition of a type-I collagen sheath to the surface of poly(ε-caprolactone) (PCL) nanofibers would enhance viability, proliferation and osteogenesis of hASCs. This is the first study to examine the differentiation behavior of hASCs on collagen-PCL sheath-core bicomponent nanofiber scaffolds developed using a co-axial electrospinning technique. The use of a sheath-core configuration ensured a uniform coating of collagen on the PCL nanofibers. PCL nanofiber scaffolds prepared using a conventional electrospinning technique served as controls. hASCs were seeded at a density of 20 000 cells/cm(2) on 1 cm(2) electrospun nanofiber (pure PCL or collagen-PCL sheath-core) sheets. Confocal microscopy and hASC proliferation data confirmed the presence of viable cells after 2 weeks in culture on all scaffolds. Greater cell spreading occurred on bicomponent collagen-PCL scaffolds at earlier time points. hASCs were osteogenically differentiated by addition of soluble osteogenic inductive factors. Calcium quantification indicated cell-mediated calcium accretion was approx. 5-times higher on bicomponent collagen-PCL sheath-core scaffolds compared to PCL controls, indicating collagen-PCL bicomponent scaffolds promoted greater hASC osteogenesis after two weeks of culture in osteogenic medium. This is the first study to examine the effects of collagen-PCL sheath-core composite nanofibers on hASC viability, proliferation and osteogenesis. The sheath-core composite fibers significantly increased calcium accretion of hASCs, indicating that collagen-PCL sheath-core bicomponent structures have potential for bone tissue engineering applications using hASCs.

Authors+Show Affiliations

Joint Department of Biomedical Engineering, North Carolina State University, Raleigh, NC 27695, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20836922

Citation

Haslauer, Carla Maria, et al. "Collagen-PCL Sheath-core Bicomponent Electrospun Scaffolds Increase Osteogenic Differentiation and Calcium Accretion of Human Adipose-derived Stem Cells." Journal of Biomaterials Science. Polymer Edition, vol. 22, no. 13, 2011, pp. 1695-712.
Haslauer CM, Moghe AK, Osborne JA, et al. Collagen-PCL sheath-core bicomponent electrospun scaffolds increase osteogenic differentiation and calcium accretion of human adipose-derived stem cells. J Biomater Sci Polym Ed. 2011;22(13):1695-712.
Haslauer, C. M., Moghe, A. K., Osborne, J. A., Gupta, B. S., & Loboa, E. G. (2011). Collagen-PCL sheath-core bicomponent electrospun scaffolds increase osteogenic differentiation and calcium accretion of human adipose-derived stem cells. Journal of Biomaterials Science. Polymer Edition, 22(13), 1695-712. https://doi.org/10.1163/092050610X521595
Haslauer CM, et al. Collagen-PCL Sheath-core Bicomponent Electrospun Scaffolds Increase Osteogenic Differentiation and Calcium Accretion of Human Adipose-derived Stem Cells. J Biomater Sci Polym Ed. 2011;22(13):1695-712. PubMed PMID: 20836922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Collagen-PCL sheath-core bicomponent electrospun scaffolds increase osteogenic differentiation and calcium accretion of human adipose-derived stem cells. AU - Haslauer,Carla Maria, AU - Moghe,Ajit K, AU - Osborne,Jason A, AU - Gupta,Bhupender S, AU - Loboa,Elizabeth G, Y1 - 2010/09/10/ PY - 2010/9/15/entrez PY - 2010/9/15/pubmed PY - 2011/11/8/medline SP - 1695 EP - 712 JF - Journal of biomaterials science. Polymer edition JO - J Biomater Sci Polym Ed VL - 22 IS - 13 N2 - Human adipose-derived stem cells (hASCs) are an abundant cell source capable of osteogenic differentiation, and have been investigated as an autologous stem cell source for bone tissue engineering applications. The objective of this study was to determine if the addition of a type-I collagen sheath to the surface of poly(ε-caprolactone) (PCL) nanofibers would enhance viability, proliferation and osteogenesis of hASCs. This is the first study to examine the differentiation behavior of hASCs on collagen-PCL sheath-core bicomponent nanofiber scaffolds developed using a co-axial electrospinning technique. The use of a sheath-core configuration ensured a uniform coating of collagen on the PCL nanofibers. PCL nanofiber scaffolds prepared using a conventional electrospinning technique served as controls. hASCs were seeded at a density of 20 000 cells/cm(2) on 1 cm(2) electrospun nanofiber (pure PCL or collagen-PCL sheath-core) sheets. Confocal microscopy and hASC proliferation data confirmed the presence of viable cells after 2 weeks in culture on all scaffolds. Greater cell spreading occurred on bicomponent collagen-PCL scaffolds at earlier time points. hASCs were osteogenically differentiated by addition of soluble osteogenic inductive factors. Calcium quantification indicated cell-mediated calcium accretion was approx. 5-times higher on bicomponent collagen-PCL sheath-core scaffolds compared to PCL controls, indicating collagen-PCL bicomponent scaffolds promoted greater hASC osteogenesis after two weeks of culture in osteogenic medium. This is the first study to examine the effects of collagen-PCL sheath-core composite nanofibers on hASC viability, proliferation and osteogenesis. The sheath-core composite fibers significantly increased calcium accretion of hASCs, indicating that collagen-PCL sheath-core bicomponent structures have potential for bone tissue engineering applications using hASCs. SN - 1568-5624 UR - https://www.unboundmedicine.com/medline/citation/20836922/Collagen_PCL_sheath_core_bicomponent_electrospun_scaffolds_increase_osteogenic_differentiation_and_calcium_accretion_of_human_adipose_derived_stem_cells_ L2 - http://www.tandfonline.com/doi/full/10.1163/092050610X521595 DB - PRIME DP - Unbound Medicine ER -