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Antioxidant and hepatoprotective activities of Egyptian moraceous plants against carbon tetrachloride-induced oxidative stress and liver damage in rats.
Pharm Biol. 2010 Nov; 48(11):1255-64.PB

Abstract

CONTEXT

In the absence of reliable liver-protective drugs in modern medicine, a large number of medicinal preparations are recommended for treatment of liver disorders.

OBJECTIVE

The antioxidant, hepatoprotective and kidney protective activities of methanol extracts of Ficus carica Linn. (Moraceae) leaves and fruits and Morus alba Linn. root barks (Moraceae) are evaluated here.

MATERIALS AND METHODS

Liver and kidney damage were induced in rats by carbon tetrachloride in a subcutaneous dose of 1 mL (40% v/v in corn oil)/kg. The extract was given intraperitoneally at doses of 50 mg/kg (F. carica leaf and M. alba root bark) and 150 mg/kg (F. carica fruit). The activity of the extracts was comparable to that of silymarin, a known hepatoprotective agent. Antioxidant activity was evaluated by measuring blood glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde equivalent (MDA). Hepatoprotective activity was evaluated by measuring serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, and total protein. These biochemical observations were supported by histopathological examination of liver sections. Kidney function was evaluated by measuring plasma urea and creatinine.

RESULTS

Methanol extracts of Ficus carica and Morus alba showed potent antioxidant and hepatoprotective activities; in-depth chromatographic investigation of the most active extract (Ficus carica leaf extract) resulted in identification of umbelliferone, caffeic acid, quercetin-3-O-β-d-glucopyranoside, quercetin-3-O-α-l-rhamnopyranoside, and kaempferol-3-O-α-l-rhamnopyranoside.

DISCUSSION AND CONCLUSION

These findings demonstrate that the phenolic constituents of Ficus carica leaf and Morus alba root bark are responsible at least in part for the observed protective effects.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Singab AN
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt. nasersingab@hotmail.com
Ayoub NA
No affiliation info available
Ali EN
No affiliation info available
Mostafa NM
No affiliation info available

MeSH

AnimalsAntioxidantsCarbon TetrachlorideChemical and Drug Induced Liver InjuryDisease Models, AnimalDose-Response Relationship, DrugEgyptFemaleFruitInjections, IntraperitonealLipid PeroxidationLiverMethanolMoraceaeOxidative StressPlant BarkPlant ExtractsPlant LeavesPlant RootsRatsSilymarin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20839909

Citation

Singab, Abdel Nasser B., et al. "Antioxidant and Hepatoprotective Activities of Egyptian Moraceous Plants Against Carbon Tetrachloride-induced Oxidative Stress and Liver Damage in Rats." Pharmaceutical Biology, vol. 48, no. 11, 2010, pp. 1255-64.
Singab AN, Ayoub NA, Ali EN, et al. Antioxidant and hepatoprotective activities of Egyptian moraceous plants against carbon tetrachloride-induced oxidative stress and liver damage in rats. Pharm Biol. 2010;48(11):1255-64.
Singab, A. N., Ayoub, N. A., Ali, E. N., & Mostafa, N. M. (2010). Antioxidant and hepatoprotective activities of Egyptian moraceous plants against carbon tetrachloride-induced oxidative stress and liver damage in rats. Pharmaceutical Biology, 48(11), 1255-64. https://doi.org/10.3109/13880201003730659
Singab AN, et al. Antioxidant and Hepatoprotective Activities of Egyptian Moraceous Plants Against Carbon Tetrachloride-induced Oxidative Stress and Liver Damage in Rats. Pharm Biol. 2010;48(11):1255-64. PubMed PMID: 20839909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and hepatoprotective activities of Egyptian moraceous plants against carbon tetrachloride-induced oxidative stress and liver damage in rats. AU - Singab,Abdel Nasser B, AU - Ayoub,Nahla A, AU - Ali,Eman N, AU - Mostafa,Nada M, Y1 - 2010/09/14/ PY - 2010/9/16/entrez PY - 2010/9/16/pubmed PY - 2011/3/9/medline SP - 1255 EP - 64 JF - Pharmaceutical biology JO - Pharm Biol VL - 48 IS - 11 N2 - CONTEXT: In the absence of reliable liver-protective drugs in modern medicine, a large number of medicinal preparations are recommended for treatment of liver disorders. OBJECTIVE: The antioxidant, hepatoprotective and kidney protective activities of methanol extracts of Ficus carica Linn. (Moraceae) leaves and fruits and Morus alba Linn. root barks (Moraceae) are evaluated here. MATERIALS AND METHODS: Liver and kidney damage were induced in rats by carbon tetrachloride in a subcutaneous dose of 1 mL (40% v/v in corn oil)/kg. The extract was given intraperitoneally at doses of 50 mg/kg (F. carica leaf and M. alba root bark) and 150 mg/kg (F. carica fruit). The activity of the extracts was comparable to that of silymarin, a known hepatoprotective agent. Antioxidant activity was evaluated by measuring blood glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde equivalent (MDA). Hepatoprotective activity was evaluated by measuring serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, and total protein. These biochemical observations were supported by histopathological examination of liver sections. Kidney function was evaluated by measuring plasma urea and creatinine. RESULTS: Methanol extracts of Ficus carica and Morus alba showed potent antioxidant and hepatoprotective activities; in-depth chromatographic investigation of the most active extract (Ficus carica leaf extract) resulted in identification of umbelliferone, caffeic acid, quercetin-3-O-β-d-glucopyranoside, quercetin-3-O-α-l-rhamnopyranoside, and kaempferol-3-O-α-l-rhamnopyranoside. DISCUSSION AND CONCLUSION: These findings demonstrate that the phenolic constituents of Ficus carica leaf and Morus alba root bark are responsible at least in part for the observed protective effects. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/20839909/Antioxidant_and_hepatoprotective_activities_of_Egyptian_moraceous_plants_against_carbon_tetrachloride_induced_oxidative_stress_and_liver_damage_in_rats_ DB - PRIME DP - Unbound Medicine ER -