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The in vitro antimicrobial activity of fruit and leaf crude extracts of Momordica charantia: a Tanzania medicinal plant.
Afr Health Sci. 2009 Mar; 9(1):34-9.AH

Abstract

OBJECTIVE

To evaluate the antimicrobial activity of Momordica charantia extracts on reference strains and microorganisms isolated from clinical specimens.

METHOD

Petroleum ether and methanolic crude extracts of fruits and leaves of the plant were evaluated for antimicrobial activity using the disk diffusion method on four reference microorganisms (Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Staphylococcus aureus; and four clinical strains of Klebsiella pneumoniae, Proteus vulgaris, Salmonella typhi and Cryptococcus neoformans.

RESULT

Antimicrobial activity was observed against all the tested microorganisms with exception to P. mirabilis and C. neoformans. Methanolic crude extracts exhibited relatively broader antimicrobial spectrum of activity than petroleum ether extracts with the as lower concentration as 0.075mg/µl. Methanolic fruit crude extract displayed the broadest antimicrobial spectrum by inhibiting majority (75%) of the tested microorganisms. Neither was there synergistic nor addition effect upon mixing leaf and fruit extracts of equal concentrations derived from the same solvent.

CONCLUSION

Extracts of M.charantia demonstrated antimicrobial activity on tested microorganisms except on Proteus mirabilis and Cryptococcus neoformans. Fruit extracts showed higher antimicrobial activity than leaf extract. Further studies are recommended that will involve various parts of the plant, select different fractions of extracts and purify the active antimicrobial components.

Authors+Show Affiliations

Muhimbili University of Health & Allied Sciences, School of Pharmacy, Department of Pharmaceutical Microbiology, P.O. Box 65013, Dar es Salaam,Tanzania. kmwambete@muhas.ac.tz.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20842240

Citation

Mwambete, K D.. "The in Vitro Antimicrobial Activity of Fruit and Leaf Crude Extracts of Momordica Charantia: a Tanzania Medicinal Plant." African Health Sciences, vol. 9, no. 1, 2009, pp. 34-9.
Mwambete KD. The in vitro antimicrobial activity of fruit and leaf crude extracts of Momordica charantia: a Tanzania medicinal plant. Afr Health Sci. 2009;9(1):34-9.
Mwambete, K. D. (2009). The in vitro antimicrobial activity of fruit and leaf crude extracts of Momordica charantia: a Tanzania medicinal plant. African Health Sciences, 9(1), 34-9.
Mwambete KD. The in Vitro Antimicrobial Activity of Fruit and Leaf Crude Extracts of Momordica Charantia: a Tanzania Medicinal Plant. Afr Health Sci. 2009;9(1):34-9. PubMed PMID: 20842240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The in vitro antimicrobial activity of fruit and leaf crude extracts of Momordica charantia: a Tanzania medicinal plant. A1 - Mwambete,K D, PY - 2010/9/16/entrez PY - 2009/3/1/pubmed PY - 2010/9/24/medline KW - Antimicrobial activity KW - Momordica charantia KW - petroleum ether and methanolic crude extracts SP - 34 EP - 9 JF - African health sciences JO - Afr Health Sci VL - 9 IS - 1 N2 - OBJECTIVE: To evaluate the antimicrobial activity of Momordica charantia extracts on reference strains and microorganisms isolated from clinical specimens. METHOD: Petroleum ether and methanolic crude extracts of fruits and leaves of the plant were evaluated for antimicrobial activity using the disk diffusion method on four reference microorganisms (Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Staphylococcus aureus; and four clinical strains of Klebsiella pneumoniae, Proteus vulgaris, Salmonella typhi and Cryptococcus neoformans. RESULT: Antimicrobial activity was observed against all the tested microorganisms with exception to P. mirabilis and C. neoformans. Methanolic crude extracts exhibited relatively broader antimicrobial spectrum of activity than petroleum ether extracts with the as lower concentration as 0.075mg/µl. Methanolic fruit crude extract displayed the broadest antimicrobial spectrum by inhibiting majority (75%) of the tested microorganisms. Neither was there synergistic nor addition effect upon mixing leaf and fruit extracts of equal concentrations derived from the same solvent. CONCLUSION: Extracts of M.charantia demonstrated antimicrobial activity on tested microorganisms except on Proteus mirabilis and Cryptococcus neoformans. Fruit extracts showed higher antimicrobial activity than leaf extract. Further studies are recommended that will involve various parts of the plant, select different fractions of extracts and purify the active antimicrobial components. SN - 1729-0503 UR - https://www.unboundmedicine.com/medline/citation/20842240/The_in_vitro_antimicrobial_activity_of_fruit_and_leaf_crude_extracts_of_Momordica_charantia:_a_Tanzania_medicinal_plant_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20842240/ DB - PRIME DP - Unbound Medicine ER -