Prime

Type your tag names separated by a space and hit enter

GC-MS analysis and antileishmanial activities of two Turkish propolis types.

Abstract

Propolis is a honeybee product with a very complex chemical composition and various pharmacological properties. This study was aimed to investigate antileishmanial activities of "Bursa" and "Hatay" propolis samples against Leishmania infantum and Leishmania tropica strains. Propolis samples were analysed with the gas chromatography-mass spectrometry technique. Promastigotes were incubated in Roswell Park Memorial Institute culture medium in the absence and presence of several concentrations (50, 100, 250, 500, 750, and 1,000 μg/mL) of each propolis sample. The viability and cell morphology of promastigotes in each concentration were examined after 24, 48, 72, and 96 h of incubation. The growth of leishmania parasites was significantly suppressed in the presence of 500, 750, and 1,000 μg/mL of Hatay propolis. Bursa propolis was found to be efficient in inhibiting the growth of leishmania promastigotes in culture media at these concentrations, 250, 500, 750, and 1,000 μg/mL. Thus, the in vitro results showed that the Hatay and Bursa propolis samples decreased significantly the proliferation of L. infantum and L. tropica parasites (p < 0.001); however, Bursa propolis was found to be more effective than Hatay propolis against leishmania promastigotes. These two natural products may be useful agents in the prevention of leishmanial infections.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Medical Faculty, Department of Microbiology and Clinical Microbiology, Mustafa Kemal University, Antakya, Turkey. nizamduran@hotmail.com

    , , ,

    Source

    Parasitology research 108:1 2011 Jan pg 95-105

    MeSH

    Animals
    Antiprotozoal Agents
    Bees
    Cell Survival
    Gas Chromatography-Mass Spectrometry
    Leishmania infantum
    Leishmania tropica
    Propolis
    Turkey

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    20842509

    Citation

    TY - JOUR T1 - GC-MS analysis and antileishmanial activities of two Turkish propolis types. AU - Duran,Nizami, AU - Muz,Mustafa, AU - Culha,Gulnaz, AU - Duran,Gulay, AU - Ozer,Burcin, Y1 - 2010/09/15/ PY - 2010/5/3/received PY - 2010/8/26/accepted PY - 2010/9/15/aheadofprint PY - 2010/9/16/entrez PY - 2010/9/16/pubmed PY - 2011/4/13/medline SP - 95 EP - 105 JF - Parasitology research JO - Parasitol. Res. VL - 108 IS - 1 N2 - Propolis is a honeybee product with a very complex chemical composition and various pharmacological properties. This study was aimed to investigate antileishmanial activities of "Bursa" and "Hatay" propolis samples against Leishmania infantum and Leishmania tropica strains. Propolis samples were analysed with the gas chromatography-mass spectrometry technique. Promastigotes were incubated in Roswell Park Memorial Institute culture medium in the absence and presence of several concentrations (50, 100, 250, 500, 750, and 1,000 μg/mL) of each propolis sample. The viability and cell morphology of promastigotes in each concentration were examined after 24, 48, 72, and 96 h of incubation. The growth of leishmania parasites was significantly suppressed in the presence of 500, 750, and 1,000 μg/mL of Hatay propolis. Bursa propolis was found to be efficient in inhibiting the growth of leishmania promastigotes in culture media at these concentrations, 250, 500, 750, and 1,000 μg/mL. Thus, the in vitro results showed that the Hatay and Bursa propolis samples decreased significantly the proliferation of L. infantum and L. tropica parasites (p < 0.001); however, Bursa propolis was found to be more effective than Hatay propolis against leishmania promastigotes. These two natural products may be useful agents in the prevention of leishmanial infections. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/20842509/abstract/GC_MS_analysis_and_antileishmanial_activities_of_two_Turkish_propolis_types_ L2 - http://dx.doi.org/10.1007/s00436-010-2039-z ER -