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In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA.
Mol Hum Reprod. 2011 Jan; 17(1):63-70.MH

Abstract

Human small antral follicles (diameter 3-9 mm) were obtained from ovaries surgically removed for fertility preservation. From the individual aspirated follicles, granulosa cells and the corresponding follicular fluid were isolated in 64 follicles, of which 55 were available for mRNA analysis (24 women). Expressions of androgen receptor (AR) mRNA levels in granulosa cells, and of androstenedione and testosterone in follicular fluid, were correlated to the expression of the FSH receptor (FSHR), LH receptor (LHR), CYP19 and anti-Müllerian Hormone-receptor II (AMHRII) mRNA in the granulosa cells and to the follicular fluid concentrations of AMH, inhibin-B, progesterone and estradiol. AR mRNA expression in granulosa cells and the follicular fluid content of androgens both showed a highly significant positive association with the expression of FSHR mRNA in granulosa cells. AR mRNA expression also correlated significantly with the expression of AMHRII, but did not correlate with any of the hormones in the follicular fluid. These data demonstrate an intimate association between AR expression in immature granulosa cells, and the expression of FSHR in normal small human antral follicles and between the follicular fluid levels of androgen and FSHR expression. This suggests that follicular sensitivity towards FSH stimulation may be augmented by stimulation of androgens via the AR.

Authors+Show Affiliations

The Fertility Clinic, Odense University Hospital, Odense , Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20843821

Citation

Nielsen, M E., et al. "In Human Granulosa Cells From Small Antral Follicles, Androgen Receptor mRNA and Androgen Levels in Follicular Fluid Correlate With FSH Receptor MRNA." Molecular Human Reproduction, vol. 17, no. 1, 2011, pp. 63-70.
Nielsen ME, Rasmussen IA, Kristensen SG, et al. In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA. Mol Hum Reprod. 2011;17(1):63-70.
Nielsen, M. E., Rasmussen, I. A., Kristensen, S. G., Christensen, S. T., Møllgård, K., Wreford Andersen, E., Byskov, A. G., & Yding Andersen, C. (2011). In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA. Molecular Human Reproduction, 17(1), 63-70. https://doi.org/10.1093/molehr/gaq073
Nielsen ME, et al. In Human Granulosa Cells From Small Antral Follicles, Androgen Receptor mRNA and Androgen Levels in Follicular Fluid Correlate With FSH Receptor MRNA. Mol Hum Reprod. 2011;17(1):63-70. PubMed PMID: 20843821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA. AU - Nielsen,M E, AU - Rasmussen,I A, AU - Kristensen,S G, AU - Christensen,S T, AU - Møllgård,K, AU - Wreford Andersen,E, AU - Byskov,A G, AU - Yding Andersen,C, Y1 - 2010/09/14/ PY - 2010/9/17/entrez PY - 2010/9/17/pubmed PY - 2011/4/16/medline SP - 63 EP - 70 JF - Molecular human reproduction JO - Mol Hum Reprod VL - 17 IS - 1 N2 - Human small antral follicles (diameter 3-9 mm) were obtained from ovaries surgically removed for fertility preservation. From the individual aspirated follicles, granulosa cells and the corresponding follicular fluid were isolated in 64 follicles, of which 55 were available for mRNA analysis (24 women). Expressions of androgen receptor (AR) mRNA levels in granulosa cells, and of androstenedione and testosterone in follicular fluid, were correlated to the expression of the FSH receptor (FSHR), LH receptor (LHR), CYP19 and anti-Müllerian Hormone-receptor II (AMHRII) mRNA in the granulosa cells and to the follicular fluid concentrations of AMH, inhibin-B, progesterone and estradiol. AR mRNA expression in granulosa cells and the follicular fluid content of androgens both showed a highly significant positive association with the expression of FSHR mRNA in granulosa cells. AR mRNA expression also correlated significantly with the expression of AMHRII, but did not correlate with any of the hormones in the follicular fluid. These data demonstrate an intimate association between AR expression in immature granulosa cells, and the expression of FSHR in normal small human antral follicles and between the follicular fluid levels of androgen and FSHR expression. This suggests that follicular sensitivity towards FSH stimulation may be augmented by stimulation of androgens via the AR. SN - 1460-2407 UR - https://www.unboundmedicine.com/medline/citation/20843821/In_human_granulosa_cells_from_small_antral_follicles_androgen_receptor_mRNA_and_androgen_levels_in_follicular_fluid_correlate_with_FSH_receptor_mRNA_ L2 - https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gaq073 DB - PRIME DP - Unbound Medicine ER -