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Distribution of non-locus of enterocyte effacement pathogenic island-related genes in Escherichia coli carrying eae from patients with diarrhea and healthy individuals in Japan.
J Clin Microbiol. 2010 Nov; 48(11):4107-14.JC

Abstract

The relationship to diarrhea of genes located on the pathogenicity islands (PAI) other than the locus of enterocyte effacement (LEE) was investigated. Enteropathogenic Escherichia coli (EPEC), the retention of espC on the EspC PAI, the OI-122 genes (efa1/lifA, nleB), the phylogenetic marker gene yjaA, and the bundle-forming pilus gene bfpA on the EPEC adherence factor (EAF) plasmid were studied. E. coli strains carrying the intimin gene (eae) without the Shiga toxin gene, isolated from patients with diarrhea (n = 83) and healthy individuals (n = 38) in Japan, were evaluated using PCR. The genotypes of eae and espC were identified by heteroduplex mobility assay (HMA). The proportions of strains isolated from individuals with and without diarrhea that carried these genes were as follows: bfpA, 13.3 and 7.9%, respectively; espC, 25.3 and 36.8%; efa1/lifA, 32.5 and 13.2%; nleB, 63.9 and 60.5%; yjaA, 42.2 and 55.3%. Statistical significance (P < 0.05) was achieved only for efa1/lifA. The proportion of strains lacking espC and carrying efa1/lifA was higher for patient-derived strains (30.1%) than for strains from healthy individuals (13.2%), but the difference was not significant. Strains carrying both espC and efa1/lifA were rare (2 strains from patients). Statistical analyses revealed significant relationships between espC and yjaA and between efa1/lifA and nleB, as well as significant inverse relationships between espC and efa1/lifA and between efa1/lifA and yjaA. espC was found in eae HMA types a1, a2, and c2, whereas efa1/lifA was found in types b1, b2, and c1. In addition, 6 polymorphisms of espC were found. The espC, yjaA, efa1/lifA, and nleB genes were mutually dependent, and their distributions were related to eae type, findings that should be considered in future epidemiological studies.

Authors+Show Affiliations

Laboratory of Microbiology, Oita Prefectural Institute of Health and Environment, Department of Public Health, Faculty of Medicine, Oita University, Oita 870-1117, Japan. narimatsu-hiroshi@pref.oita.lg.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20844211

Citation

Narimatsu, Hiroshi, et al. "Distribution of Non-locus of Enterocyte Effacement Pathogenic Island-related Genes in Escherichia Coli Carrying Eae From Patients With Diarrhea and Healthy Individuals in Japan." Journal of Clinical Microbiology, vol. 48, no. 11, 2010, pp. 4107-14.
Narimatsu H, Ogata K, Makino Y, et al. Distribution of non-locus of enterocyte effacement pathogenic island-related genes in Escherichia coli carrying eae from patients with diarrhea and healthy individuals in Japan. J Clin Microbiol. 2010;48(11):4107-14.
Narimatsu, H., Ogata, K., Makino, Y., & Ito, K. (2010). Distribution of non-locus of enterocyte effacement pathogenic island-related genes in Escherichia coli carrying eae from patients with diarrhea and healthy individuals in Japan. Journal of Clinical Microbiology, 48(11), 4107-14. https://doi.org/10.1128/JCM.00677-10
Narimatsu H, et al. Distribution of Non-locus of Enterocyte Effacement Pathogenic Island-related Genes in Escherichia Coli Carrying Eae From Patients With Diarrhea and Healthy Individuals in Japan. J Clin Microbiol. 2010;48(11):4107-14. PubMed PMID: 20844211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of non-locus of enterocyte effacement pathogenic island-related genes in Escherichia coli carrying eae from patients with diarrhea and healthy individuals in Japan. AU - Narimatsu,Hiroshi, AU - Ogata,Kikuyo, AU - Makino,Yoshihiro, AU - Ito,Kenitiro, Y1 - 2010/09/15/ PY - 2010/9/17/entrez PY - 2010/9/17/pubmed PY - 2011/2/11/medline SP - 4107 EP - 14 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 48 IS - 11 N2 - The relationship to diarrhea of genes located on the pathogenicity islands (PAI) other than the locus of enterocyte effacement (LEE) was investigated. Enteropathogenic Escherichia coli (EPEC), the retention of espC on the EspC PAI, the OI-122 genes (efa1/lifA, nleB), the phylogenetic marker gene yjaA, and the bundle-forming pilus gene bfpA on the EPEC adherence factor (EAF) plasmid were studied. E. coli strains carrying the intimin gene (eae) without the Shiga toxin gene, isolated from patients with diarrhea (n = 83) and healthy individuals (n = 38) in Japan, were evaluated using PCR. The genotypes of eae and espC were identified by heteroduplex mobility assay (HMA). The proportions of strains isolated from individuals with and without diarrhea that carried these genes were as follows: bfpA, 13.3 and 7.9%, respectively; espC, 25.3 and 36.8%; efa1/lifA, 32.5 and 13.2%; nleB, 63.9 and 60.5%; yjaA, 42.2 and 55.3%. Statistical significance (P < 0.05) was achieved only for efa1/lifA. The proportion of strains lacking espC and carrying efa1/lifA was higher for patient-derived strains (30.1%) than for strains from healthy individuals (13.2%), but the difference was not significant. Strains carrying both espC and efa1/lifA were rare (2 strains from patients). Statistical analyses revealed significant relationships between espC and yjaA and between efa1/lifA and nleB, as well as significant inverse relationships between espC and efa1/lifA and between efa1/lifA and yjaA. espC was found in eae HMA types a1, a2, and c2, whereas efa1/lifA was found in types b1, b2, and c1. In addition, 6 polymorphisms of espC were found. The espC, yjaA, efa1/lifA, and nleB genes were mutually dependent, and their distributions were related to eae type, findings that should be considered in future epidemiological studies. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/20844211/Distribution_of_non_locus_of_enterocyte_effacement_pathogenic_island_related_genes_in_Escherichia_coli_carrying_eae_from_patients_with_diarrhea_and_healthy_individuals_in_Japan_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&amp;pmid=20844211 DB - PRIME DP - Unbound Medicine ER -