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New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised.
J Assoc Physicians India. 2010 Mar; 58:147-9.JA

Abstract

BACKGROUND

Carbapenems are among the few useful antibiotics against multidrug resistant gram negative bacteria particularly those with extended spectrum beta lactamase. However resistance to carbapenems occurs and is mediated by mechanisms like loss of outer membrane proteins and production of beta lactamase that is capable of hydrolyzing carbapenems. An alert issued in the UK in 2009 warned of an increasing number of carbapenem resistant Enterobacteriaceae strains identified in UK hospital patients. Many of them were recently hospitalized in India and Pakistan and had new type of metallo beta lactamase designated as New Delhi Metallo-1 (NDM-1).

OBJECTIVE

To assess the production of NDM-1 type Metallo beta lactamase enzyme in Enterobacteriaceae at a tertiary care centre in Mumbai.

MATERIALS AND METHODS

Consecutive carbapenem resistant Enterobacteriaceae isolates were collected from August 2009 to November 2009. Susceptibility testing for carbapenems was performed by the disc diffusion method. Carbapenemase production was confirmed by Modified Hodge test. These strains were then subjected to single target PCR. A 475bp product was amplified by the NDM primers and visualized on 3% agarose gel.

RESULTS AND CONCLUSIONS

Modified Hodge test was positive for all carbapenem resistant isolates. Of 24 carbapenem resistant Enterobacteriaceae 22 were NDM producers while 2 were NDM non producers. Amongst the 22 NDM producing organisms 10 were Klebsiella spp, 9 were Escherichia coli, 2 were Enterobacter spp and 1 was Morganella morganii. This high number in a relatively short span is a worrisome trend that compromises the treatment options with the carbapenems.

Authors+Show Affiliations

Dept of Research, P.D.Hinduja National Hospital & Medical Research Centre, Mumbai, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20848811

Citation

Deshpande, Payal, et al. "New Delhi Metallo-beta Lactamase (NDM-1) in Enterobacteriaceae: Treatment Options With Carbapenems Compromised." The Journal of the Association of Physicians of India, vol. 58, 2010, pp. 147-9.
Deshpande P, Rodrigues C, Shetty A, et al. New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised. J Assoc Physicians India. 2010;58:147-9.
Deshpande, P., Rodrigues, C., Shetty, A., Kapadia, F., Hedge, A., & Soman, R. (2010). New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised. The Journal of the Association of Physicians of India, 58, 147-9.
Deshpande P, et al. New Delhi Metallo-beta Lactamase (NDM-1) in Enterobacteriaceae: Treatment Options With Carbapenems Compromised. J Assoc Physicians India. 2010;58:147-9. PubMed PMID: 20848811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised. AU - Deshpande,Payal, AU - Rodrigues,Camilla, AU - Shetty,Anjali, AU - Kapadia,Farhad, AU - Hedge,Ashit, AU - Soman,Rajeev, PY - 2010/9/21/entrez PY - 2010/9/21/pubmed PY - 2010/10/6/medline SP - 147 EP - 9 JF - The Journal of the Association of Physicians of India JO - J Assoc Physicians India VL - 58 N2 - BACKGROUND: Carbapenems are among the few useful antibiotics against multidrug resistant gram negative bacteria particularly those with extended spectrum beta lactamase. However resistance to carbapenems occurs and is mediated by mechanisms like loss of outer membrane proteins and production of beta lactamase that is capable of hydrolyzing carbapenems. An alert issued in the UK in 2009 warned of an increasing number of carbapenem resistant Enterobacteriaceae strains identified in UK hospital patients. Many of them were recently hospitalized in India and Pakistan and had new type of metallo beta lactamase designated as New Delhi Metallo-1 (NDM-1). OBJECTIVE: To assess the production of NDM-1 type Metallo beta lactamase enzyme in Enterobacteriaceae at a tertiary care centre in Mumbai. MATERIALS AND METHODS: Consecutive carbapenem resistant Enterobacteriaceae isolates were collected from August 2009 to November 2009. Susceptibility testing for carbapenems was performed by the disc diffusion method. Carbapenemase production was confirmed by Modified Hodge test. These strains were then subjected to single target PCR. A 475bp product was amplified by the NDM primers and visualized on 3% agarose gel. RESULTS AND CONCLUSIONS: Modified Hodge test was positive for all carbapenem resistant isolates. Of 24 carbapenem resistant Enterobacteriaceae 22 were NDM producers while 2 were NDM non producers. Amongst the 22 NDM producing organisms 10 were Klebsiella spp, 9 were Escherichia coli, 2 were Enterobacter spp and 1 was Morganella morganii. This high number in a relatively short span is a worrisome trend that compromises the treatment options with the carbapenems. SN - 0004-5772 UR - https://www.unboundmedicine.com/medline/citation/20848811/New_Delhi_Metallo_beta_lactamase__NDM_1__in_Enterobacteriaceae:_treatment_options_with_carbapenems_compromised_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -