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[Effects of electroacupuncture plus oxygenmedicine on the expression of Bcl-2 and Bax proteins in the hippocampal CA 1 area in rats with global cerebral ischemia/reperfusion injury].
Zhen Ci Yan Jiu. 2010 Jun; 35(3):208-12.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) plus oxygenmedicine (OM) on the expression of Bcl-2 and Bax in the hippocampal CA 1 area in cerebral ischemia/reperfusion injury (CI/RI) rats.

METHODS

Thirty SD rats were randomized into sham-operation, model, EA, OM, EA+OM groups (n=6 /group). CI/RI model was established by using modified Pulsinelli 4 vessel occlusion and reperfusion. EA (100 Hz, 3.5 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) 30 min, once daily for 4 days. Rats of OM and EA+ OM groups were put into a box filled with oxygen and atomized herbal medicines containing Bingpian (Borneolum), Shexiang (Moschus), Huangjing (Rhizoma Polygonati), Shouwu (Radix Polygoni Multiflori), etc. for 30 min, once daily for 4 days. Bcl-2 and Bax expression of the hippocampal CA 1 area was detected by immunohistochemistry.

RESULTS

Compared with sham group, the numbers of Bcl-2 immunoreaction (IR) and Bax IR positive cells, and the immunoactivity of Bcl-2 IR and Bax IR positive products in the hippocampal CA 1 area were increased significantly in model group (P < 0.05, P < 0.01). In comparison with model group, the number of Bcl-2 IR positive cells and Bcl-2 immunoactivity in EA, OM and EA+OM groups increased considerably (P < 0.01), while Bax IR positive cell numbers and Bax immunoactivity in EA, OM and OM+ EA groups decreased significantly (P < 0.01). The effects of EA+ OM were significantly superior to those of EA and OM groups in upregulating Bcl-2 IR positive cell number and Bcl-2 immunoactivity and downregulating Bax IR positive cell number and Bax immunoactivity (P < 0.01). No significant differences were found between EA and OM groups in the abovemen-EA and OM and EA+OM can effectively regulate the expression of Bcl-2 and Bax in tioned indexes (P > 0.05).

CONCLUSION

EA and OM and EA + OM can effectively regulate the expression of Bcl-2 and Bax in the hippocampal CA 1 area in CI/RI rats, and the effects of EA+OM are significantly superior to those of simple EA and simple OM, which may contribute to their effect in improving cerebral ischemia.

Authors+Show Affiliations

Department of Neurology, Xuzhou Hospital of Chinese Medicine, Xuzhou 221000, China. acupuncturebu@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

20848897

Citation

Bu, Yuan, et al. "[Effects of Electroacupuncture Plus Oxygenmedicine On the Expression of Bcl-2 and Bax Proteins in the Hippocampal CA 1 Area in Rats With Global Cerebral Ischemia/reperfusion Injury]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 35, no. 3, 2010, pp. 208-12.
Bu Y, Zhang PY, Geng DQ, et al. [Effects of electroacupuncture plus oxygenmedicine on the expression of Bcl-2 and Bax proteins in the hippocampal CA 1 area in rats with global cerebral ischemia/reperfusion injury]. Zhen Ci Yan Jiu. 2010;35(3):208-12.
Bu, Y., Zhang, P. Y., Geng, D. Q., Cao, J. X., & Li, J. Z. (2010). [Effects of electroacupuncture plus oxygenmedicine on the expression of Bcl-2 and Bax proteins in the hippocampal CA 1 area in rats with global cerebral ischemia/reperfusion injury]. Zhen Ci Yan Jiu = Acupuncture Research, 35(3), 208-12.
Bu Y, et al. [Effects of Electroacupuncture Plus Oxygenmedicine On the Expression of Bcl-2 and Bax Proteins in the Hippocampal CA 1 Area in Rats With Global Cerebral Ischemia/reperfusion Injury]. Zhen Ci Yan Jiu. 2010;35(3):208-12. PubMed PMID: 20848897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture plus oxygenmedicine on the expression of Bcl-2 and Bax proteins in the hippocampal CA 1 area in rats with global cerebral ischemia/reperfusion injury]. AU - Bu,Yuan, AU - Zhang,Pei-ying, AU - Geng,De-qin, AU - Cao,Jin-xia, AU - Li,Ji-zhen, PY - 2010/9/21/entrez PY - 2010/9/21/pubmed PY - 2010/10/6/medline SP - 208 EP - 12 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 35 IS - 3 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) plus oxygenmedicine (OM) on the expression of Bcl-2 and Bax in the hippocampal CA 1 area in cerebral ischemia/reperfusion injury (CI/RI) rats. METHODS: Thirty SD rats were randomized into sham-operation, model, EA, OM, EA+OM groups (n=6 /group). CI/RI model was established by using modified Pulsinelli 4 vessel occlusion and reperfusion. EA (100 Hz, 3.5 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) 30 min, once daily for 4 days. Rats of OM and EA+ OM groups were put into a box filled with oxygen and atomized herbal medicines containing Bingpian (Borneolum), Shexiang (Moschus), Huangjing (Rhizoma Polygonati), Shouwu (Radix Polygoni Multiflori), etc. for 30 min, once daily for 4 days. Bcl-2 and Bax expression of the hippocampal CA 1 area was detected by immunohistochemistry. RESULTS: Compared with sham group, the numbers of Bcl-2 immunoreaction (IR) and Bax IR positive cells, and the immunoactivity of Bcl-2 IR and Bax IR positive products in the hippocampal CA 1 area were increased significantly in model group (P < 0.05, P < 0.01). In comparison with model group, the number of Bcl-2 IR positive cells and Bcl-2 immunoactivity in EA, OM and EA+OM groups increased considerably (P < 0.01), while Bax IR positive cell numbers and Bax immunoactivity in EA, OM and OM+ EA groups decreased significantly (P < 0.01). The effects of EA+ OM were significantly superior to those of EA and OM groups in upregulating Bcl-2 IR positive cell number and Bcl-2 immunoactivity and downregulating Bax IR positive cell number and Bax immunoactivity (P < 0.01). No significant differences were found between EA and OM groups in the abovemen-EA and OM and EA+OM can effectively regulate the expression of Bcl-2 and Bax in tioned indexes (P > 0.05). CONCLUSION: EA and OM and EA + OM can effectively regulate the expression of Bcl-2 and Bax in the hippocampal CA 1 area in CI/RI rats, and the effects of EA+OM are significantly superior to those of simple EA and simple OM, which may contribute to their effect in improving cerebral ischemia. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/20848897/[Effects_of_electroacupuncture_plus_oxygenmedicine_on_the_expression_of_Bcl_2_and_Bax_proteins_in_the_hippocampal_CA_1_area_in_rats_with_global_cerebral_ischemia/reperfusion_injury]_ L2 - https://antibodies.cancer.gov/detail/CPTC-BAX-1 DB - PRIME DP - Unbound Medicine ER -