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Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein.
Antiviral Res. 2010 Nov; 88(2):236-43.AR

Abstract

Sulphated glycosaminoglycans such as heparin inhibit the early step of dengue virus infection through interaction with envelope (E) protein. Here, we found that chondroitin sulphate E (CSE), but not CSD, which contains the same degree of sulphation, inhibited dengue virus (DENV) infection of cells with adsorption. CSE significantly reduced infectivity of all dengue virus serotypes to BHK-21 and Vero cells. DENV preferentially bound to CSE immobilised on plastic plates. Also, virus binding to CSE or heparin was cross-inhibited by soluble CSE or heparin. These findings suggested that common carbohydrate determinants on CSE and heparin could be essential epitopes for interaction of DENV, and may be responsible for inhibition of the early steps of DENV infection. A recombinant E protein directly bound heparin and CSE, but not CSD, meaning that interaction of CSE with E protein contributes to the inhibitory action of this glycosaminoglycan. These observations indicate that a specific carbohydrate structure rather than polysulphation or addition of negative charges of the glycosaminoglycan molecule would be necessary for direct binding to DENV E protein. In conclusion, CSE showed antiviral activity as an entry inhibitor targeting E protein of dengue virus.

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Authors+Show Affiliations

Kato D
Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and Global COE Program for Innovation in Human Health Sciences, 52-1 Yada, Suruga-ku, Shizuoka-shi, Shizuoka 422-8526, Japan.
Era S
No affiliation info available
Watanabe I
No affiliation info available
Arihara M
No affiliation info available
Sugiura N
No affiliation info available
Kimata K
No affiliation info available
Suzuki Y
No affiliation info available
Morita K
No affiliation info available
Hidari KI
No affiliation info available
Suzuki T
No affiliation info available

MeSH

AedesAnimalsAntiviral AgentsBinding SitesBinding, CompetitiveChlorocebus aethiopsChondroitin SulfatesDengue VirusGene ExpressionGlycosaminoglycansHeparitin SulfateHyaluronic AcidVero CellsViral Envelope ProteinsVirus AttachmentVirus Internalization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20851716

Citation

Kato, Daisuke, et al. "Antiviral Activity of Chondroitin Sulphate E Targeting Dengue Virus Envelope Protein." Antiviral Research, vol. 88, no. 2, 2010, pp. 236-43.
Kato D, Era S, Watanabe I, et al. Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein. Antiviral Res. 2010;88(2):236-43.
Kato, D., Era, S., Watanabe, I., Arihara, M., Sugiura, N., Kimata, K., Suzuki, Y., Morita, K., Hidari, K. I., & Suzuki, T. (2010). Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein. Antiviral Research, 88(2), 236-43. https://doi.org/10.1016/j.antiviral.2010.09.002
Kato D, et al. Antiviral Activity of Chondroitin Sulphate E Targeting Dengue Virus Envelope Protein. Antiviral Res. 2010;88(2):236-43. PubMed PMID: 20851716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiviral activity of chondroitin sulphate E targeting dengue virus envelope protein. AU - Kato,Daisuke, AU - Era,Shota, AU - Watanabe,Ippei, AU - Arihara,Masataka, AU - Sugiura,Nobuo, AU - Kimata,Koji, AU - Suzuki,Yasuo, AU - Morita,Kouichi, AU - Hidari,Kazuya I P J, AU - Suzuki,Takashi, Y1 - 2010/09/21/ PY - 2010/07/10/received PY - 2010/08/23/revised PY - 2010/09/02/accepted PY - 2010/9/21/entrez PY - 2010/9/21/pubmed PY - 2010/12/31/medline SP - 236 EP - 43 JF - Antiviral research JO - Antiviral Res VL - 88 IS - 2 N2 - Sulphated glycosaminoglycans such as heparin inhibit the early step of dengue virus infection through interaction with envelope (E) protein. Here, we found that chondroitin sulphate E (CSE), but not CSD, which contains the same degree of sulphation, inhibited dengue virus (DENV) infection of cells with adsorption. CSE significantly reduced infectivity of all dengue virus serotypes to BHK-21 and Vero cells. DENV preferentially bound to CSE immobilised on plastic plates. Also, virus binding to CSE or heparin was cross-inhibited by soluble CSE or heparin. These findings suggested that common carbohydrate determinants on CSE and heparin could be essential epitopes for interaction of DENV, and may be responsible for inhibition of the early steps of DENV infection. A recombinant E protein directly bound heparin and CSE, but not CSD, meaning that interaction of CSE with E protein contributes to the inhibitory action of this glycosaminoglycan. These observations indicate that a specific carbohydrate structure rather than polysulphation or addition of negative charges of the glycosaminoglycan molecule would be necessary for direct binding to DENV E protein. In conclusion, CSE showed antiviral activity as an entry inhibitor targeting E protein of dengue virus. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/20851716/Antiviral_activity_of_chondroitin_sulphate_E_targeting_dengue_virus_envelope_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(10)00706-0 DB - PRIME DP - Unbound Medicine ER -