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Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species.
Insect Mol Biol. 2011 Feb; 20(1):1-13.IM

Abstract

Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monopetala Roxb. using in vitro proteolytic assays, zymogram analyses with proteinase inhibitors, restriction digestion of RNA-PCR amplicons and quantitative real-time PCR (RT-PCR). Eight novel members of the serine proteinase gene family were identified, including an intron-spliced trypsin (AaPb4) and seven putative chymotrypsins (AaPb2, AaPb4, AaPb12, AaLm4, AaLm6, AaLm19 and AaLm29). Midgut transcript levels of the putative trypsin were higher in larvae fed P. bombycina whereas levels of transcripts encoding putative chymotrypsins were higher in larvae reared on L. monopetala. Complex, differential expression of sequence divergent midgut serine proteinases may reflect the ability of lepidopteran larvae to feed on different species of host plants. Possible implications of host plant choice on the digestive physiology of A. assamensis are discussed.

Authors+Show Affiliations

Insect-Plant Interactions Group, Department of Botany, University of Delhi, Delhi, India. mahaswetta@yahoo.co.inNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20854480

Citation

Saikia, M, et al. "Expression of Diverse Midgut Serine Proteinases in the Sericigenous Lepidoptera Antheraea Assamensis (Helfer) Is Influenced By Choice of Host Plant Species." Insect Molecular Biology, vol. 20, no. 1, 2011, pp. 1-13.
Saikia M, Singh YT, Bhattacharya A, et al. Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species. Insect Mol Biol. 2011;20(1):1-13.
Saikia, M., Singh, Y. T., Bhattacharya, A., & Mazumdar-Leighton, S. (2011). Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species. Insect Molecular Biology, 20(1), 1-13. https://doi.org/10.1111/j.1365-2583.2010.01048.x
Saikia M, et al. Expression of Diverse Midgut Serine Proteinases in the Sericigenous Lepidoptera Antheraea Assamensis (Helfer) Is Influenced By Choice of Host Plant Species. Insect Mol Biol. 2011;20(1):1-13. PubMed PMID: 20854480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species. AU - Saikia,M, AU - Singh,Y T, AU - Bhattacharya,A, AU - Mazumdar-Leighton,S, Y1 - 2010/09/21/ PY - 2010/9/22/entrez PY - 2010/9/22/pubmed PY - 2011/4/8/medline SP - 1 EP - 13 JF - Insect molecular biology JO - Insect Mol Biol VL - 20 IS - 1 N2 - Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monopetala Roxb. using in vitro proteolytic assays, zymogram analyses with proteinase inhibitors, restriction digestion of RNA-PCR amplicons and quantitative real-time PCR (RT-PCR). Eight novel members of the serine proteinase gene family were identified, including an intron-spliced trypsin (AaPb4) and seven putative chymotrypsins (AaPb2, AaPb4, AaPb12, AaLm4, AaLm6, AaLm19 and AaLm29). Midgut transcript levels of the putative trypsin were higher in larvae fed P. bombycina whereas levels of transcripts encoding putative chymotrypsins were higher in larvae reared on L. monopetala. Complex, differential expression of sequence divergent midgut serine proteinases may reflect the ability of lepidopteran larvae to feed on different species of host plants. Possible implications of host plant choice on the digestive physiology of A. assamensis are discussed. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/20854480/Expression_of_diverse_midgut_serine_proteinases_in_the_sericigenous_Lepidoptera_Antheraea_assamensis__Helfer__is_influenced_by_choice_of_host_plant_species_ L2 - https://doi.org/10.1111/j.1365-2583.2010.01048.x DB - PRIME DP - Unbound Medicine ER -