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A hybrid liquid chromatography-mass spectrometry strategy in a forensic laboratory for opioid, cocaine and amphetamine classes in human urine using a hybrid linear ion trap-triple quadrupole mass spectrometer.
J Chromatogr A. 2010 Oct 29; 1217(44):6857-66.JC

Abstract

A rapid method has been developed to analyse morphine, codeine, morphine-3-glucuronide, 6-monoacetylmorphine, cocaine, benzoylegonine, buprenorphine, dihydrocodeine, cocaethylene, 3,4-methylenedioxyamphetamine, ketamine, 3,4-methylenedioxymethamphetamine, pseudoephedrine, lignocaine, benzylpiperazine, methamphetamine, amphetamine, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and methadone in human urine. Urine samples were diluted with methanol:water (1:1, v/v) and sample aliquots were analysed by hybrid linear ion trap-triple quadrupole mass spectrometry with a runtime of 12.5 min. Multiple reaction monitoring (MRM) as survey scan and an enhanced product ion (EPI) scan as dependent scan were performed in an information-dependent acquisition (IDA) experiment. Finally, drug identification and confirmation was carried out by library search with a developed in-house MS/MS library based on EPI spectra at a collision energy spread of 35±15 in positive mode and MRM ratios. The method was validated in urine, according to the criteria defined in Commission Decision 2002/657/EC. At least two MRM transitions for each substance were monitored in addition to EPI spectra and deuterated analytes were used as internal standards for quantitation. The reporting level was 0.05 μg mL(-1) for the range of analytes tested. The regression coefficients (r(2)) for the calibration curves (0-4 μg mL(-1)) in the study were ≥0.98. The method proved to be simple and time efficient and was implemented as an analytical strategy for the illicit drug monitoring of opioids, cocaines and amphetamines in criminal samples from crime offenders, abusers or victims in the Republic of Ireland. To the best of our knowledge there are no hybrid LC-MS applications using MRM mode and product ion spectra in the linear ion trap mode for opioids, cocaines or amphetamines with validation data in urine.

Authors+Show Affiliations

The State Laboratory, Backweston Laboratory Complex, Young's Cross, Celbridge, Co. Kildare, Ireland. Geraldine.Dowling@statelab.ieNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20855077

Citation

Dowling, Geraldine, et al. "A Hybrid Liquid Chromatography-mass Spectrometry Strategy in a Forensic Laboratory for Opioid, Cocaine and Amphetamine Classes in Human Urine Using a Hybrid Linear Ion Trap-triple Quadrupole Mass Spectrometer." Journal of Chromatography. A, vol. 1217, no. 44, 2010, pp. 6857-66.
Dowling G, Regan L, Tierney J, et al. A hybrid liquid chromatography-mass spectrometry strategy in a forensic laboratory for opioid, cocaine and amphetamine classes in human urine using a hybrid linear ion trap-triple quadrupole mass spectrometer. J Chromatogr A. 2010;1217(44):6857-66.
Dowling, G., Regan, L., Tierney, J., & Nangle, M. (2010). A hybrid liquid chromatography-mass spectrometry strategy in a forensic laboratory for opioid, cocaine and amphetamine classes in human urine using a hybrid linear ion trap-triple quadrupole mass spectrometer. Journal of Chromatography. A, 1217(44), 6857-66. https://doi.org/10.1016/j.chroma.2010.08.043
Dowling G, et al. A Hybrid Liquid Chromatography-mass Spectrometry Strategy in a Forensic Laboratory for Opioid, Cocaine and Amphetamine Classes in Human Urine Using a Hybrid Linear Ion Trap-triple Quadrupole Mass Spectrometer. J Chromatogr A. 2010 Oct 29;1217(44):6857-66. PubMed PMID: 20855077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A hybrid liquid chromatography-mass spectrometry strategy in a forensic laboratory for opioid, cocaine and amphetamine classes in human urine using a hybrid linear ion trap-triple quadrupole mass spectrometer. AU - Dowling,Geraldine, AU - Regan,Liam, AU - Tierney,Julie, AU - Nangle,Michael, Y1 - 2010/09/18/ PY - 2010/04/15/received PY - 2010/08/03/revised PY - 2010/08/16/accepted PY - 2010/9/22/entrez PY - 2010/9/22/pubmed PY - 2011/1/25/medline SP - 6857 EP - 66 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 44 N2 - A rapid method has been developed to analyse morphine, codeine, morphine-3-glucuronide, 6-monoacetylmorphine, cocaine, benzoylegonine, buprenorphine, dihydrocodeine, cocaethylene, 3,4-methylenedioxyamphetamine, ketamine, 3,4-methylenedioxymethamphetamine, pseudoephedrine, lignocaine, benzylpiperazine, methamphetamine, amphetamine, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and methadone in human urine. Urine samples were diluted with methanol:water (1:1, v/v) and sample aliquots were analysed by hybrid linear ion trap-triple quadrupole mass spectrometry with a runtime of 12.5 min. Multiple reaction monitoring (MRM) as survey scan and an enhanced product ion (EPI) scan as dependent scan were performed in an information-dependent acquisition (IDA) experiment. Finally, drug identification and confirmation was carried out by library search with a developed in-house MS/MS library based on EPI spectra at a collision energy spread of 35±15 in positive mode and MRM ratios. The method was validated in urine, according to the criteria defined in Commission Decision 2002/657/EC. At least two MRM transitions for each substance were monitored in addition to EPI spectra and deuterated analytes were used as internal standards for quantitation. The reporting level was 0.05 μg mL(-1) for the range of analytes tested. The regression coefficients (r(2)) for the calibration curves (0-4 μg mL(-1)) in the study were ≥0.98. The method proved to be simple and time efficient and was implemented as an analytical strategy for the illicit drug monitoring of opioids, cocaines and amphetamines in criminal samples from crime offenders, abusers or victims in the Republic of Ireland. To the best of our knowledge there are no hybrid LC-MS applications using MRM mode and product ion spectra in the linear ion trap mode for opioids, cocaines or amphetamines with validation data in urine. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20855077/A_hybrid_liquid_chromatography_mass_spectrometry_strategy_in_a_forensic_laboratory_for_opioid_cocaine_and_amphetamine_classes_in_human_urine_using_a_hybrid_linear_ion_trap_triple_quadrupole_mass_spectrometer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)01115-5 DB - PRIME DP - Unbound Medicine ER -