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Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections.
Virol J. 2010 Sep 22; 7:249.VJ

Abstract

BACKGROUND

Differential diagnose of Japanese encephalitis virus (JEV) infection from other flavivirus especially West Nile virus (WNV) and Dengue virus (DV) infection was greatly hindered for the serological cross-reactive. Virus specific epitopes could benefit for developing JEV specific antibodies detection methods. To identify the JEV specific epitopes, we fully mapped and characterized the continuous B-cell epitope of the PrM/M protein of JEV.

RESULTS

To map the epitopes on the PrM/M protein, we designed a set of 20 partially overlapping fragments spanning the whole PrM, fused them with GST, and expressed them in an expression vector. Linear epitope M14 (105VNKKEAWLDSTKATRY120) was detected by enzyme-linked immunosorbent assay (ELISA). By removing amino acid residues individually from the carboxy and amino terminal of peptide M14, we confirmed that the minimal unit of the linear epitope of PrM/M was M14-13 (108KEAWLDSTKAT118). This epitope was highly conserved across different JEV strains. Moreover, this epitope did not cross-react with WNV-positive and DENV-positive sera.

CONCLUSION

Epitope M14-13 was a JEV specific lineal B-cell epitpe. The results may provide a useful basis for the development of epitope-based virus specific diagnostic clinical techniques.

Authors+Show Affiliations

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20858291

Citation

Hua, Rong-Hong, et al. "Identification and Characterization of a Virus-specific Continuous B-cell Epitope On the PrM/M Protein of Japanese Encephalitis Virus: Potential Application in the Detection of Antibodies to Distinguish Japanese Encephalitis Virus Infection From West Nile Virus and Dengue Virus Infections." Virology Journal, vol. 7, 2010, p. 249.
Hua RH, Chen NS, Qin CF, et al. Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections. Virol J. 2010;7:249.
Hua, R. H., Chen, N. S., Qin, C. F., Deng, Y. Q., Ge, J. Y., Wang, X. J., Qiao, Z. J., Chen, W. Y., Wen, Z. Y., Liu, W. X., Hu, S., & Bu, Z. G. (2010). Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections. Virology Journal, 7, 249. https://doi.org/10.1186/1743-422X-7-249
Hua RH, et al. Identification and Characterization of a Virus-specific Continuous B-cell Epitope On the PrM/M Protein of Japanese Encephalitis Virus: Potential Application in the Detection of Antibodies to Distinguish Japanese Encephalitis Virus Infection From West Nile Virus and Dengue Virus Infections. Virol J. 2010 Sep 22;7:249. PubMed PMID: 20858291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections. AU - Hua,Rong-Hong, AU - Chen,Na-Sha, AU - Qin,Cheng-Feng, AU - Deng,Yong-Qiang, AU - Ge,Jin-Ying, AU - Wang,Xi-Jun, AU - Qiao,Zu-Jian, AU - Chen,Wei-Ye, AU - Wen,Zhi-Yuan, AU - Liu,Wen-Xin, AU - Hu,Sen, AU - Bu,Zhi-Gao, Y1 - 2010/09/22/ PY - 2010/08/25/received PY - 2010/09/22/accepted PY - 2010/9/23/entrez PY - 2010/9/23/pubmed PY - 2010/12/14/medline SP - 249 EP - 249 JF - Virology journal JO - Virol J VL - 7 N2 - BACKGROUND: Differential diagnose of Japanese encephalitis virus (JEV) infection from other flavivirus especially West Nile virus (WNV) and Dengue virus (DV) infection was greatly hindered for the serological cross-reactive. Virus specific epitopes could benefit for developing JEV specific antibodies detection methods. To identify the JEV specific epitopes, we fully mapped and characterized the continuous B-cell epitope of the PrM/M protein of JEV. RESULTS: To map the epitopes on the PrM/M protein, we designed a set of 20 partially overlapping fragments spanning the whole PrM, fused them with GST, and expressed them in an expression vector. Linear epitope M14 (105VNKKEAWLDSTKATRY120) was detected by enzyme-linked immunosorbent assay (ELISA). By removing amino acid residues individually from the carboxy and amino terminal of peptide M14, we confirmed that the minimal unit of the linear epitope of PrM/M was M14-13 (108KEAWLDSTKAT118). This epitope was highly conserved across different JEV strains. Moreover, this epitope did not cross-react with WNV-positive and DENV-positive sera. CONCLUSION: Epitope M14-13 was a JEV specific lineal B-cell epitpe. The results may provide a useful basis for the development of epitope-based virus specific diagnostic clinical techniques. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/20858291/Identification_and_characterization_of_a_virus_specific_continuous_B_cell_epitope_on_the_PrM/M_protein_of_Japanese_Encephalitis_Virus:_potential_application_in_the_detection_of_antibodies_to_distinguish_Japanese_Encephalitis_Virus_infection_from_West_Nile_Virus_and_Dengue_Virus_infections_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-7-249 DB - PRIME DP - Unbound Medicine ER -