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Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars.
J Genet. 2010 Aug; 89(2):193-9.JG

Abstract

Proline and glutamine-rich wheat seed endosperm proteins are collectively referred to as prolamins. They are comprised of HMW-GSs, LMW-GSs and gliadins. HMW-GSs are major determinants of gluten elasticity and LMW-GSs considerably affect dough extensibility and maximum dough resistance. The inheritance of glutenin subunits follows Mendelian genetics with multiple alleles in each locus. Identification of the banding patterns of glutenin subunits could be used as an estimate for screening high quality wheat germplasm. Here, by means of a two-step 1D-SDS-PAGE procedure, we identified the allelic variations in high and low-molecular-weight glutenin subunits in 65 hexaploid wheat (Triticum aestivum L.) cultivars representing a historical trend in the cultivars introduced or released in Iran from the years 1940 to 1990. Distinct alleles 17 and 19 were detected for Glu-1 and Glu-3 loci, respectively. The allelic frequencies at the Glu-1 loci demonstrated unimodal distributions. At Glu-A1, Glu-B1 and Glu-D1, we found that the most frequent alleles were the null, 7 + 8, 2 + 12 alleles, respectively, in Iranian wheat cultivars. In contrast, Glu-3 loci showed bimodal or trimodal distributions. At Glu-A3, the most frequent alleles were c and e. At Glu-B3 the most frequent alleles were a, b and c. At Glu-D3 locus, the alleles b and a, were the most and the second most frequent alleles in Iranian wheat cultivars. This led to a significantly higher Nei coefficient of genetic variations in Glu-3 loci (0.756) as compared to Glu-1 loci (0.547). At Glu-3 loci, we observed relatively high quality alleles in Glu-A3 and Glu-D3 loci and low quality alleles at Glu-B3 locus.

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Authors+Show Affiliations

Izadi-Darbandi A
Department of Agronomy and Plant Breeding, University of Tehran, Iran.
Yazdi-Samadi B
No affiliation info available
Shanejat-Boushehri AA
No affiliation info available
Mohammadi M
No affiliation info available

MeSH

AllelesElectrophoresis, Gel, Two-DimensionalGenes, PlantGenetic VariationGlutensIranPhylogenyTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20861570

Citation

Izadi-Darbandi, Ali, et al. "Allelic Variations in Glu-1 and Glu-3 Loci of Historical and Modern Iranian Bread Wheat (Triticum Aestivum L.) Cultivars." Journal of Genetics, vol. 89, no. 2, 2010, pp. 193-9.
Izadi-Darbandi A, Yazdi-Samadi B, Shanejat-Boushehri AA, et al. Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars. J Genet. 2010;89(2):193-9.
Izadi-Darbandi, A., Yazdi-Samadi, B., Shanejat-Boushehri, A. A., & Mohammadi, M. (2010). Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars. Journal of Genetics, 89(2), 193-9.
Izadi-Darbandi A, et al. Allelic Variations in Glu-1 and Glu-3 Loci of Historical and Modern Iranian Bread Wheat (Triticum Aestivum L.) Cultivars. J Genet. 2010;89(2):193-9. PubMed PMID: 20861570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars. AU - Izadi-Darbandi,Ali, AU - Yazdi-Samadi,Bahman, AU - Shanejat-Boushehri,Ali-Akbar, AU - Mohammadi,Mohsen, PY - 2010/9/24/entrez PY - 2010/9/24/pubmed PY - 2011/2/18/medline SP - 193 EP - 9 JF - Journal of genetics JO - J Genet VL - 89 IS - 2 N2 - Proline and glutamine-rich wheat seed endosperm proteins are collectively referred to as prolamins. They are comprised of HMW-GSs, LMW-GSs and gliadins. HMW-GSs are major determinants of gluten elasticity and LMW-GSs considerably affect dough extensibility and maximum dough resistance. The inheritance of glutenin subunits follows Mendelian genetics with multiple alleles in each locus. Identification of the banding patterns of glutenin subunits could be used as an estimate for screening high quality wheat germplasm. Here, by means of a two-step 1D-SDS-PAGE procedure, we identified the allelic variations in high and low-molecular-weight glutenin subunits in 65 hexaploid wheat (Triticum aestivum L.) cultivars representing a historical trend in the cultivars introduced or released in Iran from the years 1940 to 1990. Distinct alleles 17 and 19 were detected for Glu-1 and Glu-3 loci, respectively. The allelic frequencies at the Glu-1 loci demonstrated unimodal distributions. At Glu-A1, Glu-B1 and Glu-D1, we found that the most frequent alleles were the null, 7 + 8, 2 + 12 alleles, respectively, in Iranian wheat cultivars. In contrast, Glu-3 loci showed bimodal or trimodal distributions. At Glu-A3, the most frequent alleles were c and e. At Glu-B3 the most frequent alleles were a, b and c. At Glu-D3 locus, the alleles b and a, were the most and the second most frequent alleles in Iranian wheat cultivars. This led to a significantly higher Nei coefficient of genetic variations in Glu-3 loci (0.756) as compared to Glu-1 loci (0.547). At Glu-3 loci, we observed relatively high quality alleles in Glu-A3 and Glu-D3 loci and low quality alleles at Glu-B3 locus. SN - 0973-7731 UR - https://www.unboundmedicine.com/medline/citation/20861570/Allelic_variations_in_Glu_1_and_Glu_3_loci_of_historical_and_modern_Iranian_bread_wheat__Triticum_aestivum_L___cultivars_ L2 - https://www.ias.ac.in/article/fulltext/jgen/089/02/0193 DB - PRIME DP - Unbound Medicine ER -