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Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles.
J Mass Spectrom. 2010 Sep; 45(9):966-70.JM

Abstract

Two Raboso cultivars, i.e. Raboso Veronese and Raboso Piave, are two black Vitis vinifera varieties. A genetic study suggested that Raboso Veronese is the progeny of a spontaneous cross between Raboso Piave and Marzemina Bianca cultivars. Parental relationships are usually investigated by genetic studies, which are effective to establish genetic links among different cultivars. Considering that proteome is the genome expression, in this article we evaluated the power of seed protein profiles obtained by matrix-assisted laser desorption/ionization (MALDI)/MS for parentage investigation. The three cultivars lead to very similar spectra with differences in the relative intensity of the most abundant species and the presence of very weak specific ions. In order to evaluate the analytical significance of these aspects, the variability due to instrumental factors and due to different harvesting areas and years of the same cultivars have been considered and measured by the calculation of discrepancy factor values. On one hand, the results obtained can be considered a valid confirmation of the genomic findings, whereas on the other hand, the results provide evidence for the ability of MALDI/MS to individuate minor differences in protein profiles of complex protein mixtures.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bertazzo A
Dipartimento di Scienze Farmaceutiche, Universita' di Padova, Via Francesco Marzolo 5, 35131 Padova, Italy. antonella.bertazzo@unipd.it
De Rosso M
No affiliation info available
Dalla Vedova A
No affiliation info available
Agnolin F
No affiliation info available
Flamini R
No affiliation info available
Traldi P
No affiliation info available

MeSH

Reproducibility of ResultsSeed Storage ProteinsSeedsSpecies SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationVitis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20862730

Citation

Bertazzo, Antonella, et al. "Parental Relationships Among Three Grape Varieties Studied By MALDI of Grape Seed Protein Profiles." Journal of Mass Spectrometry : JMS, vol. 45, no. 9, 2010, pp. 966-70.
Bertazzo A, De Rosso M, Dalla Vedova A, et al. Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles. J Mass Spectrom. 2010;45(9):966-70.
Bertazzo, A., De Rosso, M., Dalla Vedova, A., Agnolin, F., Flamini, R., & Traldi, P. (2010). Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles. Journal of Mass Spectrometry : JMS, 45(9), 966-70. https://doi.org/10.1002/jms.1738
Bertazzo A, et al. Parental Relationships Among Three Grape Varieties Studied By MALDI of Grape Seed Protein Profiles. J Mass Spectrom. 2010;45(9):966-70. PubMed PMID: 20862730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles. AU - Bertazzo,Antonella, AU - De Rosso,Mirko, AU - Dalla Vedova,Antonio, AU - Agnolin,Fabio, AU - Flamini,Riccardo, AU - Traldi,Pietro, PY - 2010/9/24/entrez PY - 2010/9/24/pubmed PY - 2011/1/11/medline SP - 966 EP - 70 JF - Journal of mass spectrometry : JMS JO - J Mass Spectrom VL - 45 IS - 9 N2 - Two Raboso cultivars, i.e. Raboso Veronese and Raboso Piave, are two black Vitis vinifera varieties. A genetic study suggested that Raboso Veronese is the progeny of a spontaneous cross between Raboso Piave and Marzemina Bianca cultivars. Parental relationships are usually investigated by genetic studies, which are effective to establish genetic links among different cultivars. Considering that proteome is the genome expression, in this article we evaluated the power of seed protein profiles obtained by matrix-assisted laser desorption/ionization (MALDI)/MS for parentage investigation. The three cultivars lead to very similar spectra with differences in the relative intensity of the most abundant species and the presence of very weak specific ions. In order to evaluate the analytical significance of these aspects, the variability due to instrumental factors and due to different harvesting areas and years of the same cultivars have been considered and measured by the calculation of discrepancy factor values. On one hand, the results obtained can be considered a valid confirmation of the genomic findings, whereas on the other hand, the results provide evidence for the ability of MALDI/MS to individuate minor differences in protein profiles of complex protein mixtures. SN - 1096-9888 UR - https://www.unboundmedicine.com/medline/citation/20862730/Parental_relationships_among_three_grape_varieties_studied_by_MALDI_of_grape_seed_protein_profiles_ DB - PRIME DP - Unbound Medicine ER -