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Enhancing the lateral-flow immunoassay for viral detection using an aqueous two-phase micellar system.
Anal Bioanal Chem. 2010 Dec; 398(7-8):2955-61.AB

Abstract

Availability of a rapid, accurate, and reliable point-of-care (POC) device for detection of infectious agents and pandemic pathogens, such as swine-origin influenza A (H1N1) virus, is crucial for effective patient management and outbreak prevention. Due to its ease of use, rapid processing, and minimal power and laboratory equipment requirements, the lateral-flow (immuno)assay (LFA) has gained much attention in recent years as a possible solution. However, since the sensitivity of LFA has been shown to be inferior to that of the gold standards of pathogen detection, namely cell culture and real-time PCR, LFA remains an ineffective POC assay for preventing pandemic outbreaks. A practical solution for increasing the sensitivity of LFA is to concentrate the target agent in a solution prior to the detection step. In this study, an aqueous two-phase micellar system comprised of the nonionic surfactant Triton X-114 was investigated for concentrating a model virus, namely bacteriophage M13 (M13), prior to LFA. The volume ratio of the two coexisting micellar phases was manipulated to concentrate M13 in the top, micelle-poor phase. The concentration step effectively improved the M13 detection limit of the assay by tenfold from 5 × 10(8) plaque forming units (pfu)/mL to 5 × 10(7) pfu/mL. In the future, the volume ratio can be further manipulated to yield a greater concentration of a target virus and further decrease the detection limits of the LFA.

Authors+Show Affiliations

Department of Bioengineering, University of California, 5121 Engineering V, 420 Westwood Plaza, Los Angeles, CA 90095-1600, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20865404

Citation

Mashayekhi, Foad, et al. "Enhancing the Lateral-flow Immunoassay for Viral Detection Using an Aqueous Two-phase Micellar System." Analytical and Bioanalytical Chemistry, vol. 398, no. 7-8, 2010, pp. 2955-61.
Mashayekhi F, Chiu RY, Le AM, et al. Enhancing the lateral-flow immunoassay for viral detection using an aqueous two-phase micellar system. Anal Bioanal Chem. 2010;398(7-8):2955-61.
Mashayekhi, F., Chiu, R. Y., Le, A. M., Chao, F. C., Wu, B. M., & Kamei, D. T. (2010). Enhancing the lateral-flow immunoassay for viral detection using an aqueous two-phase micellar system. Analytical and Bioanalytical Chemistry, 398(7-8), 2955-61. https://doi.org/10.1007/s00216-010-4213-7
Mashayekhi F, et al. Enhancing the Lateral-flow Immunoassay for Viral Detection Using an Aqueous Two-phase Micellar System. Anal Bioanal Chem. 2010;398(7-8):2955-61. PubMed PMID: 20865404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancing the lateral-flow immunoassay for viral detection using an aqueous two-phase micellar system. AU - Mashayekhi,Foad, AU - Chiu,Ricky Y T, AU - Le,Alexander M, AU - Chao,Felix C, AU - Wu,Benjamin M, AU - Kamei,Daniel T, Y1 - 2010/09/24/ PY - 2010/07/23/received PY - 2010/09/08/accepted PY - 2010/09/07/revised PY - 2010/9/25/entrez PY - 2010/9/25/pubmed PY - 2011/4/8/medline SP - 2955 EP - 61 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 398 IS - 7-8 N2 - Availability of a rapid, accurate, and reliable point-of-care (POC) device for detection of infectious agents and pandemic pathogens, such as swine-origin influenza A (H1N1) virus, is crucial for effective patient management and outbreak prevention. Due to its ease of use, rapid processing, and minimal power and laboratory equipment requirements, the lateral-flow (immuno)assay (LFA) has gained much attention in recent years as a possible solution. However, since the sensitivity of LFA has been shown to be inferior to that of the gold standards of pathogen detection, namely cell culture and real-time PCR, LFA remains an ineffective POC assay for preventing pandemic outbreaks. A practical solution for increasing the sensitivity of LFA is to concentrate the target agent in a solution prior to the detection step. In this study, an aqueous two-phase micellar system comprised of the nonionic surfactant Triton X-114 was investigated for concentrating a model virus, namely bacteriophage M13 (M13), prior to LFA. The volume ratio of the two coexisting micellar phases was manipulated to concentrate M13 in the top, micelle-poor phase. The concentration step effectively improved the M13 detection limit of the assay by tenfold from 5 × 10(8) plaque forming units (pfu)/mL to 5 × 10(7) pfu/mL. In the future, the volume ratio can be further manipulated to yield a greater concentration of a target virus and further decrease the detection limits of the LFA. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/20865404/Enhancing_the_lateral_flow_immunoassay_for_viral_detection_using_an_aqueous_two_phase_micellar_system_ L2 - https://dx.doi.org/10.1007/s00216-010-4213-7 DB - PRIME DP - Unbound Medicine ER -