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Puerarin attenuates amyloid-beta-induced cognitive impairment through suppression of apoptosis in rat hippocampus in vivo.

Abstract

Elevated levels of β-amyloid (Aβ) in the brains being a hallmark of Alzheimer's disease have been believed to play a critical role in the cognitive dysfunction that occurs in Alzheimer's disease. Recent evidence suggests that Aβ induces neuronal apoptosis in the brain and in primary neuronal cultures. In this study, we investigated the effects of puerarin, a phytoestrogen isolated from Pueraria lobata, on cognitive function and neuronal apoptosis in the intrahippocampal injection of Aβ rats and its mechanism of action. The results show the intrahippocampal injection of Aβ induced a spatial memory deficit, apoptosis, and caspase-9 activation in hippocampal neurons. Puerarin treatment ameliorated Aβ(1-42)-induced cognitive impairment and reversed the increase of apoptosis in the hippocampus. The attenuation is associated with the activation of Akt and phosphorylation of Bad. These results suggest that puerarin may be an anti-Alzheimer's disease candidate drug to suppress both Alzheimer's disease-related neuronal cell apoptosis and dysfunction of the memory system.

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  • Authors+Show Affiliations

    ,

    The Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China.

    , , , , , , , ,

    Source

    European journal of pharmacology 649:1-3 2010 Dec 15 pg 195-201

    MeSH

    Alzheimer Disease
    Amyloid beta-Peptides
    Animals
    Apoptosis
    Apoptosis Regulatory Proteins
    Cells, Cultured
    Cognition Disorders
    Dose-Response Relationship, Drug
    Enzyme Activation
    Gene Expression Regulation
    Hippocampus
    Isoflavones
    Male
    Maze Learning
    Memory Disorders
    Nerve Tissue Proteins
    Neurons
    Neuroprotective Agents
    Phosphorylation
    Phytoestrogens
    Proto-Oncogene Proteins c-akt
    RNA, Messenger
    Rats
    Rats, Sprague-Dawley

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20868658

    Citation

    Li, Jiaming, et al. "Puerarin Attenuates Amyloid-beta-induced Cognitive Impairment Through Suppression of Apoptosis in Rat Hippocampus in Vivo." European Journal of Pharmacology, vol. 649, no. 1-3, 2010, pp. 195-201.
    Li J, Wang G, Liu J, et al. Puerarin attenuates amyloid-beta-induced cognitive impairment through suppression of apoptosis in rat hippocampus in vivo. Eur J Pharmacol. 2010;649(1-3):195-201.
    Li, J., Wang, G., Liu, J., Zhou, L., Dong, M., Wang, R., ... Niu, Y. (2010). Puerarin attenuates amyloid-beta-induced cognitive impairment through suppression of apoptosis in rat hippocampus in vivo. European Journal of Pharmacology, 649(1-3), pp. 195-201. doi:10.1016/j.ejphar.2010.09.045.
    Li J, et al. Puerarin Attenuates Amyloid-beta-induced Cognitive Impairment Through Suppression of Apoptosis in Rat Hippocampus in Vivo. Eur J Pharmacol. 2010 Dec 15;649(1-3):195-201. PubMed PMID: 20868658.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Puerarin attenuates amyloid-beta-induced cognitive impairment through suppression of apoptosis in rat hippocampus in vivo. AU - Li,Jiaming, AU - Wang,Gang, AU - Liu,Jicheng, AU - Zhou,Li, AU - Dong,Miaoxian, AU - Wang,Rui, AU - Li,Xueyan, AU - Li,Xiaoming, AU - Lin,Chunrong, AU - Niu,Yingcai, Y1 - 2010/09/22/ PY - 2010/04/11/received PY - 2010/07/04/revised PY - 2010/09/14/accepted PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2011/3/4/medline SP - 195 EP - 201 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 649 IS - 1-3 N2 - Elevated levels of β-amyloid (Aβ) in the brains being a hallmark of Alzheimer's disease have been believed to play a critical role in the cognitive dysfunction that occurs in Alzheimer's disease. Recent evidence suggests that Aβ induces neuronal apoptosis in the brain and in primary neuronal cultures. In this study, we investigated the effects of puerarin, a phytoestrogen isolated from Pueraria lobata, on cognitive function and neuronal apoptosis in the intrahippocampal injection of Aβ rats and its mechanism of action. The results show the intrahippocampal injection of Aβ induced a spatial memory deficit, apoptosis, and caspase-9 activation in hippocampal neurons. Puerarin treatment ameliorated Aβ(1-42)-induced cognitive impairment and reversed the increase of apoptosis in the hippocampus. The attenuation is associated with the activation of Akt and phosphorylation of Bad. These results suggest that puerarin may be an anti-Alzheimer's disease candidate drug to suppress both Alzheimer's disease-related neuronal cell apoptosis and dysfunction of the memory system. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/20868658/Puerarin_attenuates_amyloid_beta_induced_cognitive_impairment_through_suppression_of_apoptosis_in_rat_hippocampus_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(10)00930-1 DB - PRIME DP - Unbound Medicine ER -