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Fetal bovine serum requirement for pyrrolidine dithiocarbamate-induced apoptotic cell death of MCF-7 breast tumor cells.
Eur J Pharmacol. 2010 Dec 15; 649(1-3):135-9.EJ

Abstract

Pyrrolidine dithiocarbamate (PDTC) can form a complex with metal ions and then act as a proteasome inhibitor, which leads to tumor cell apoptosis, and could therefore be developed as an anticancer agent. In our efforts to find factors that induce PDTC-mediated apoptosis of tumor cells, the effect of serum concentration on the apoptotic activity of PDTC was investigated. PDTC could not induce MCF-7 breast tumor cell death in serum-free media but significantly induced cell death in a dose-dependent manner at concentrations of ≥25 μM in media containing 10% fetal bovine serum. PDTC-mediated cell death was also dependent on serum concentration. PDTC-mediated cell death occurred through apoptosis. Similar to that in normal FBS, PDTC-mediated apoptotic cell death was also induced in media containing dialyzed FBS, indicating that PDTC-mediated apoptosis does not require metal ions or salts, but rather proteins in fetal bovine serum. In addition, differential apoptotic effects of PDTC were not observed with inhibitors of NF-κB activation such as N-acetylcysteine (NAC), Fenofibrate and carbobenzoxyl-l-leucyl-l-leucyl-l-leucinal (MG132) or with the metal-binding agent, 5-chloro-7-iodo-8-hydroxyquinoline (Clioquinol). These results indicate that serum is required for PDTC-mediated apoptosis and that zinc-binding compounds such as PDTC, N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) and Clioquinol may each have their own mechanisms by which they induce tumor cell death, even though they are all classified as zinc-binding compounds.

Authors+Show Affiliations

East-West Bone & Joint Research Institute, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, Gangdong-gu, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20868670

Citation

Oh, Da Hee, et al. "Fetal Bovine Serum Requirement for Pyrrolidine Dithiocarbamate-induced Apoptotic Cell Death of MCF-7 Breast Tumor Cells." European Journal of Pharmacology, vol. 649, no. 1-3, 2010, pp. 135-9.
Oh DH, Bang JS, Choi HM, et al. Fetal bovine serum requirement for pyrrolidine dithiocarbamate-induced apoptotic cell death of MCF-7 breast tumor cells. Eur J Pharmacol. 2010;649(1-3):135-9.
Oh, D. H., Bang, J. S., Choi, H. M., Yang, H. I., Yoo, M. C., & Kim, K. S. (2010). Fetal bovine serum requirement for pyrrolidine dithiocarbamate-induced apoptotic cell death of MCF-7 breast tumor cells. European Journal of Pharmacology, 649(1-3), 135-9. https://doi.org/10.1016/j.ejphar.2010.09.048
Oh DH, et al. Fetal Bovine Serum Requirement for Pyrrolidine Dithiocarbamate-induced Apoptotic Cell Death of MCF-7 Breast Tumor Cells. Eur J Pharmacol. 2010 Dec 15;649(1-3):135-9. PubMed PMID: 20868670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fetal bovine serum requirement for pyrrolidine dithiocarbamate-induced apoptotic cell death of MCF-7 breast tumor cells. AU - Oh,Da Hee, AU - Bang,Jun Soo, AU - Choi,Hyun Mi, AU - Yang,Hyung-In, AU - Yoo,Myung Chul, AU - Kim,Kyoung Soo, Y1 - 2010/09/22/ PY - 2010/07/23/received PY - 2010/09/16/accepted PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2011/3/4/medline SP - 135 EP - 9 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 649 IS - 1-3 N2 - Pyrrolidine dithiocarbamate (PDTC) can form a complex with metal ions and then act as a proteasome inhibitor, which leads to tumor cell apoptosis, and could therefore be developed as an anticancer agent. In our efforts to find factors that induce PDTC-mediated apoptosis of tumor cells, the effect of serum concentration on the apoptotic activity of PDTC was investigated. PDTC could not induce MCF-7 breast tumor cell death in serum-free media but significantly induced cell death in a dose-dependent manner at concentrations of ≥25 μM in media containing 10% fetal bovine serum. PDTC-mediated cell death was also dependent on serum concentration. PDTC-mediated cell death occurred through apoptosis. Similar to that in normal FBS, PDTC-mediated apoptotic cell death was also induced in media containing dialyzed FBS, indicating that PDTC-mediated apoptosis does not require metal ions or salts, but rather proteins in fetal bovine serum. In addition, differential apoptotic effects of PDTC were not observed with inhibitors of NF-κB activation such as N-acetylcysteine (NAC), Fenofibrate and carbobenzoxyl-l-leucyl-l-leucyl-l-leucinal (MG132) or with the metal-binding agent, 5-chloro-7-iodo-8-hydroxyquinoline (Clioquinol). These results indicate that serum is required for PDTC-mediated apoptosis and that zinc-binding compounds such as PDTC, N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) and Clioquinol may each have their own mechanisms by which they induce tumor cell death, even though they are all classified as zinc-binding compounds. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/20868670/Fetal_bovine_serum_requirement_for_pyrrolidine_dithiocarbamate_induced_apoptotic_cell_death_of_MCF_7_breast_tumor_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(10)00933-7 DB - PRIME DP - Unbound Medicine ER -