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Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Nov 01; 878(29):2943-8.JC

Abstract

High-performance liquid chromatographic (HPLC) with evaporative light scattering detection (ELSD) and HPLC with electrospray ionization multistage tandem mass spectrometry (HPLC-ESI-MS(n)) were used to identify and quantify steroid saponins in Paris and Trillium plants. The content of the known saponins such as Paris I, II, III, V, VI, VII, H, gracillin and protodioscin in Paris and Trillium plants was determined simultaneously using the developed HPLC-ELSD method. Furthermore, other 12 steroid saponins were identified by HPLC-ESI(+/-)-MS(n) detection. In the end, a developed analytical procedure was proved to be a reliable and rapid method for the quality control of Paris and Trillium plants. In addition, the alternative resources for Paris yunnanensis used as a traditional Chinese medicine were discovered according to the hierarchical clustering analysis of the saponin fraction of these plants.

Authors+Show Affiliations

School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin 300072, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20870470

Citation

Man, Shuli, et al. "Qualitative and Quantitative Determination of Major Saponins in Paris and Trillium By HPLC-ELSD and HPLC-MS/MS." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 29, 2010, pp. 2943-8.
Man S, Gao W, Zhang Y, et al. Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(29):2943-8.
Man, S., Gao, W., Zhang, Y., Wang, J., Zhao, W., Huang, L., & Liu, C. (2010). Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(29), 2943-8. https://doi.org/10.1016/j.jchromb.2010.08.033
Man S, et al. Qualitative and Quantitative Determination of Major Saponins in Paris and Trillium By HPLC-ELSD and HPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Nov 1;878(29):2943-8. PubMed PMID: 20870470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS. AU - Man,Shuli, AU - Gao,Wenyuan, AU - Zhang,Yanjun, AU - Wang,Jieyin, AU - Zhao,Wanshun, AU - Huang,Luqi, AU - Liu,Changxiao, Y1 - 2010/10/01/ PY - 2010/01/23/received PY - 2010/08/17/revised PY - 2010/08/19/accepted PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2011/1/14/medline SP - 2943 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 29 N2 - High-performance liquid chromatographic (HPLC) with evaporative light scattering detection (ELSD) and HPLC with electrospray ionization multistage tandem mass spectrometry (HPLC-ESI-MS(n)) were used to identify and quantify steroid saponins in Paris and Trillium plants. The content of the known saponins such as Paris I, II, III, V, VI, VII, H, gracillin and protodioscin in Paris and Trillium plants was determined simultaneously using the developed HPLC-ELSD method. Furthermore, other 12 steroid saponins were identified by HPLC-ESI(+/-)-MS(n) detection. In the end, a developed analytical procedure was proved to be a reliable and rapid method for the quality control of Paris and Trillium plants. In addition, the alternative resources for Paris yunnanensis used as a traditional Chinese medicine were discovered according to the hierarchical clustering analysis of the saponin fraction of these plants. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20870470/Qualitative_and_quantitative_determination_of_major_saponins_in_Paris_and_Trillium_by_HPLC_ELSD_and_HPLC_MS/MS_ DB - PRIME DP - Unbound Medicine ER -