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2-Gy whole-body irradiation significantly alters the balance of CD4+ CD25- T effector cells and CD4+ CD25+ Foxp3+ T regulatory cells in mice.
Cell Mol Immunol 2010; 7(6):419-27CM

Abstract

CD4(+)CD25(+) T regulatory (Treg) cells are critical in inducing and maintaining immunological self-tolerance as well as transplant tolerance. The effect of low doses of whole-body irradiation (WBI) on CD4(+)CD25(+)Foxp3(+) Treg cells has not been determined. The proportion, phenotypes and function of CD4(+)CD25(+) Treg cells were investigated 0.5, 5 and 15 days after euthymic, thymectomized or allogeneic bone marrow transplanted C57BL/6 mice received 2-Gy γ-rays of WBI. The 2-Gy WBI significantly enhanced the ratios of CD4(+)CD25(+) Treg cells and CD4(+)CD25(+)Foxp3(+) Treg cells to CD4(+) T cells in peripheral blood, lymph nodes, spleens and thymi of mice. The CD4(+)CD25(+) Treg cells of the WBI-treated mice showed immunosuppressive activities on the immune response of CD4(+)CD25(-) T effector cells to alloantigens or mitogens as efficiently as the control mice. Furthermore, 2-Gy γ-ray WBI significantly increased the percentage of CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery of either thymectomized mice or allogeneic bone marrow transplanted mice. The in vitro assay showed that ionizing irradiation induced less cell death in CD4(+)CD25(+)Foxp3(+) Treg cells than in CD4(+)CD25(-) T cells. Thus, a low dose of WBI could significantly enhance the level of functional CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery of naive or immunized mice. The enhanced proportion of CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery by a low dose of WBI may make hosts more susceptible to immune tolerance induction.

Authors+Show Affiliations

Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20871628

Citation

Qu, Yanyan, et al. "2-Gy Whole-body Irradiation Significantly Alters the Balance of CD4+ CD25- T Effector Cells and CD4+ CD25+ Foxp3+ T Regulatory Cells in Mice." Cellular & Molecular Immunology, vol. 7, no. 6, 2010, pp. 419-27.
Qu Y, Zhang B, Liu S, et al. 2-Gy whole-body irradiation significantly alters the balance of CD4+ CD25- T effector cells and CD4+ CD25+ Foxp3+ T regulatory cells in mice. Cell Mol Immunol. 2010;7(6):419-27.
Qu, Y., Zhang, B., Liu, S., Zhang, A., Wu, T., & Zhao, Y. (2010). 2-Gy whole-body irradiation significantly alters the balance of CD4+ CD25- T effector cells and CD4+ CD25+ Foxp3+ T regulatory cells in mice. Cellular & Molecular Immunology, 7(6), pp. 419-27. doi:10.1038/cmi.2010.45.
Qu Y, et al. 2-Gy Whole-body Irradiation Significantly Alters the Balance of CD4+ CD25- T Effector Cells and CD4+ CD25+ Foxp3+ T Regulatory Cells in Mice. Cell Mol Immunol. 2010;7(6):419-27. PubMed PMID: 20871628.
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TY - JOUR T1 - 2-Gy whole-body irradiation significantly alters the balance of CD4+ CD25- T effector cells and CD4+ CD25+ Foxp3+ T regulatory cells in mice. AU - Qu,Yanyan, AU - Zhang,Baojun, AU - Liu,Shuchun, AU - Zhang,Aijun, AU - Wu,Tingting, AU - Zhao,Yong, Y1 - 2010/09/27/ PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2011/3/3/medline SP - 419 EP - 27 JF - Cellular & molecular immunology JO - Cell. Mol. Immunol. VL - 7 IS - 6 N2 - CD4(+)CD25(+) T regulatory (Treg) cells are critical in inducing and maintaining immunological self-tolerance as well as transplant tolerance. The effect of low doses of whole-body irradiation (WBI) on CD4(+)CD25(+)Foxp3(+) Treg cells has not been determined. The proportion, phenotypes and function of CD4(+)CD25(+) Treg cells were investigated 0.5, 5 and 15 days after euthymic, thymectomized or allogeneic bone marrow transplanted C57BL/6 mice received 2-Gy γ-rays of WBI. The 2-Gy WBI significantly enhanced the ratios of CD4(+)CD25(+) Treg cells and CD4(+)CD25(+)Foxp3(+) Treg cells to CD4(+) T cells in peripheral blood, lymph nodes, spleens and thymi of mice. The CD4(+)CD25(+) Treg cells of the WBI-treated mice showed immunosuppressive activities on the immune response of CD4(+)CD25(-) T effector cells to alloantigens or mitogens as efficiently as the control mice. Furthermore, 2-Gy γ-ray WBI significantly increased the percentage of CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery of either thymectomized mice or allogeneic bone marrow transplanted mice. The in vitro assay showed that ionizing irradiation induced less cell death in CD4(+)CD25(+)Foxp3(+) Treg cells than in CD4(+)CD25(-) T cells. Thus, a low dose of WBI could significantly enhance the level of functional CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery of naive or immunized mice. The enhanced proportion of CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery by a low dose of WBI may make hosts more susceptible to immune tolerance induction. SN - 2042-0226 UR - https://www.unboundmedicine.com/medline/citation/20871628/2_Gy_whole_body_irradiation_significantly_alters_the_balance_of_CD4+_CD25__T_effector_cells_and_CD4+_CD25+_Foxp3+_T_regulatory_cells_in_mice_ L2 - http://dx.doi.org/10.1038/cmi.2010.45 DB - PRIME DP - Unbound Medicine ER -