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Improved characterization of tomato polyphenols using liquid chromatography/electrospray ionization linear ion trap quadrupole Orbitrap mass spectrometry and liquid chromatography/electrospray ionization tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2010 Oct 30; 24(20):2986-92.RC

Abstract

Tomato (Lycopersicon esculentum Mill.) is the second most important fruit crop worldwide. Tomatoes are a key component in the Mediterranean diet, which is strongly associated with a reduced risk of chronic degenerative diseases. In this work, we use a combination of mass spectrometry (MS) techniques with negative ion detection, liquid chromatography/electrospray ionization linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the constituents of tomato samples. First, we tested for the presence of polyphenolic compounds through generic MS/MS experiments such as neutral loss and precursor ion scans on the triple quadrupole system. Confirmation of the compounds previously identified was accomplished by injection into the high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. In this way, 38 compounds were identified in tomato samples with very good mass accuracy (<2 mDa), three of them, as far as we know, not previously reported in tomato samples.

Authors+Show Affiliations

Nutrition and Food Science Department, XaRTA, INSA, Pharmacy School, University of Barcelona, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20872631

Citation

Vallverdú-Queralt, Anna, et al. "Improved Characterization of Tomato Polyphenols Using Liquid Chromatography/electrospray Ionization Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry and Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 24, no. 20, 2010, pp. 2986-92.
Vallverdú-Queralt A, Jáuregui O, Medina-Remón A, et al. Improved characterization of tomato polyphenols using liquid chromatography/electrospray ionization linear ion trap quadrupole Orbitrap mass spectrometry and liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 2010;24(20):2986-92.
Vallverdú-Queralt, A., Jáuregui, O., Medina-Remón, A., Andrés-Lacueva, C., & Lamuela-Raventós, R. M. (2010). Improved characterization of tomato polyphenols using liquid chromatography/electrospray ionization linear ion trap quadrupole Orbitrap mass spectrometry and liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 24(20), 2986-92. https://doi.org/10.1002/rcm.4731
Vallverdú-Queralt A, et al. Improved Characterization of Tomato Polyphenols Using Liquid Chromatography/electrospray Ionization Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry and Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2010 Oct 30;24(20):2986-92. PubMed PMID: 20872631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved characterization of tomato polyphenols using liquid chromatography/electrospray ionization linear ion trap quadrupole Orbitrap mass spectrometry and liquid chromatography/electrospray ionization tandem mass spectrometry. AU - Vallverdú-Queralt,Anna, AU - Jáuregui,Olga, AU - Medina-Remón,Alexander, AU - Andrés-Lacueva,Cristina, AU - Lamuela-Raventós,Rosa M, PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2010/12/31/medline SP - 2986 EP - 92 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 24 IS - 20 N2 - Tomato (Lycopersicon esculentum Mill.) is the second most important fruit crop worldwide. Tomatoes are a key component in the Mediterranean diet, which is strongly associated with a reduced risk of chronic degenerative diseases. In this work, we use a combination of mass spectrometry (MS) techniques with negative ion detection, liquid chromatography/electrospray ionization linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the constituents of tomato samples. First, we tested for the presence of polyphenolic compounds through generic MS/MS experiments such as neutral loss and precursor ion scans on the triple quadrupole system. Confirmation of the compounds previously identified was accomplished by injection into the high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. In this way, 38 compounds were identified in tomato samples with very good mass accuracy (<2 mDa), three of them, as far as we know, not previously reported in tomato samples. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/20872631/Improved_characterization_of_tomato_polyphenols_using_liquid_chromatography/electrospray_ionization_linear_ion_trap_quadrupole_Orbitrap_mass_spectrometry_and_liquid_chromatography/electrospray_ionization_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -