Tags

Type your tag names separated by a space and hit enter

Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome.
Rapid Commun Mass Spectrom. 2010 Oct 30; 24(20):3021-32.RC

Abstract

Peptide tagging is a useful tool to improve matrix-assisted laser desorption/ionization tandem mass spectrometric (MALDI-MS/MS) analysis. We present a new application of the use of the dansyl chloride (DNS-Cl). DNS-Cl is a specific primary amine reagent widely used in protein biochemistry. It adds a fluorescent dimethylaminonaphthalene moiety to the molecule. The evaluation of MALDI-MS and MS/MS analyses of dansylated peptides shows that dansylation raises the ionization efficiency of the most hydrophilic species compared with the most hydrophobic ones. Consequently, higher Mascot scores and protein sequence coverage are obtained by combining MS and MS/MS data of native and tagged samples. The N-terminal DNS-Cl sulfonation improves the peptide fragmentation and promotes the generation of b-fragments allowing better peptide sequencing. In addition, we set up a labeling protocol based on the microwave chemistry. Peptide dansylation proved to be a rapid and cheap method to improve the performance of liquid chromatography (LC)/MALDI-MS/MS analysis at the proteomic scale in terms of peptide detection and sequence coverage.

Authors+Show Affiliations

USR 3149 CNRS/ESPCI ParisTech, Ecole Supérieure de Physique et de Chimie Industrielles de Paris, Paris, France. giovanni.chiappetta@unina.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20872635

Citation

Chiappetta, Giovanni, et al. "Dansyl-peptides Matrix-assisted Laser Desorption/ionization Mass Spectrometric (MALDI-MS) and Tandem Mass Spectrometric (MS/MS) Features Improve the Liquid chromatography/MALDI-MS/MS Analysis of the Proteome." Rapid Communications in Mass Spectrometry : RCM, vol. 24, no. 20, 2010, pp. 3021-32.
Chiappetta G, Ndiaye S, Demey E, et al. Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome. Rapid Commun Mass Spectrom. 2010;24(20):3021-32.
Chiappetta, G., Ndiaye, S., Demey, E., Haddad, I., Marino, G., Amoresano, A., & Vinh, J. (2010). Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome. Rapid Communications in Mass Spectrometry : RCM, 24(20), 3021-32. https://doi.org/10.1002/rcm.4734
Chiappetta G, et al. Dansyl-peptides Matrix-assisted Laser Desorption/ionization Mass Spectrometric (MALDI-MS) and Tandem Mass Spectrometric (MS/MS) Features Improve the Liquid chromatography/MALDI-MS/MS Analysis of the Proteome. Rapid Commun Mass Spectrom. 2010 Oct 30;24(20):3021-32. PubMed PMID: 20872635.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome. AU - Chiappetta,Giovanni, AU - Ndiaye,Sega, AU - Demey,Emmanuelle, AU - Haddad,Iman, AU - Marino,Gennaro, AU - Amoresano,Angela, AU - Vinh,Joëlle, PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2010/12/31/medline SP - 3021 EP - 32 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 24 IS - 20 N2 - Peptide tagging is a useful tool to improve matrix-assisted laser desorption/ionization tandem mass spectrometric (MALDI-MS/MS) analysis. We present a new application of the use of the dansyl chloride (DNS-Cl). DNS-Cl is a specific primary amine reagent widely used in protein biochemistry. It adds a fluorescent dimethylaminonaphthalene moiety to the molecule. The evaluation of MALDI-MS and MS/MS analyses of dansylated peptides shows that dansylation raises the ionization efficiency of the most hydrophilic species compared with the most hydrophobic ones. Consequently, higher Mascot scores and protein sequence coverage are obtained by combining MS and MS/MS data of native and tagged samples. The N-terminal DNS-Cl sulfonation improves the peptide fragmentation and promotes the generation of b-fragments allowing better peptide sequencing. In addition, we set up a labeling protocol based on the microwave chemistry. Peptide dansylation proved to be a rapid and cheap method to improve the performance of liquid chromatography (LC)/MALDI-MS/MS analysis at the proteomic scale in terms of peptide detection and sequence coverage. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/20872635/Dansyl_peptides_matrix_assisted_laser_desorption/ionization_mass_spectrometric__MALDI_MS__and_tandem_mass_spectrometric__MS/MS__features_improve_the_liquid_chromatography/MALDI_MS/MS_analysis_of_the_proteome_ L2 - https://doi.org/10.1002/rcm.4734 DB - PRIME DP - Unbound Medicine ER -