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Determination of pethidine in human plasma by LC-MS/MS.
Biomed Chromatogr. 2011 Jul; 25(7):833-7.BC

Abstract

A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of pethidine in human plasma was developed and validated over the concentration range of 4-2000 ng/mL. After addition of ketamine as internal standard, liquid-liquid extraction was used to produce a protein-free extract. Chromatographic separation was achieved on a 100 × 2.1 mm, 5 µm particle, Allure™ PFP propyl column, with 45:40:15 (v/v/v) acetonitrile-methanol-water containing 0.2% formic acid as mobile phase. The MS data acquisition was accomplished by multiple reactions monitoring mode with positive electrospray ionization interface. The lower limit of quantification was 4 ng/mL; for inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 7%, and the accuracy was within 95.9-106.5%. The method is sensitive and simple, and was successfully applied to analysis of samples of clinical intoxication.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang X
Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China. lankywang@yahoo.cn
Xiang Z
No affiliation info available
Cai X
No affiliation info available
Wu H
No affiliation info available
Wang X
No affiliation info available
Li J
No affiliation info available
Zhang M
No affiliation info available

MeSH

AdultChemical FractionationChromatography, LiquidDrug StabilityFemaleHumansInfantLinear ModelsMaleMeperidineReproducibility of ResultsSensitivity and SpecificityTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20872940

Citation

Wang, Xianqin, et al. "Determination of Pethidine in Human Plasma By LC-MS/MS." Biomedical Chromatography : BMC, vol. 25, no. 7, 2011, pp. 833-7.
Wang X, Xiang Z, Cai X, et al. Determination of pethidine in human plasma by LC-MS/MS. Biomed Chromatogr. 2011;25(7):833-7.
Wang, X., Xiang, Z., Cai, X., Wu, H., Wang, X., Li, J., & Zhang, M. (2011). Determination of pethidine in human plasma by LC-MS/MS. Biomedical Chromatography : BMC, 25(7), 833-7. https://doi.org/10.1002/bmc.1524
Wang X, et al. Determination of Pethidine in Human Plasma By LC-MS/MS. Biomed Chromatogr. 2011;25(7):833-7. PubMed PMID: 20872940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of pethidine in human plasma by LC-MS/MS. AU - Wang,Xianqin, AU - Xiang,Zheng, AU - Cai,Xiaojun, AU - Wu,Haiya, AU - Wang,Xuebao, AU - Li,Junwei, AU - Zhang,Meiling, Y1 - 2010/09/27/ PY - 2010/05/27/received PY - 2010/08/10/accepted PY - 2010/9/28/entrez PY - 2010/9/28/pubmed PY - 2011/10/8/medline SP - 833 EP - 7 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 25 IS - 7 N2 - A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of pethidine in human plasma was developed and validated over the concentration range of 4-2000 ng/mL. After addition of ketamine as internal standard, liquid-liquid extraction was used to produce a protein-free extract. Chromatographic separation was achieved on a 100 × 2.1 mm, 5 µm particle, Allure™ PFP propyl column, with 45:40:15 (v/v/v) acetonitrile-methanol-water containing 0.2% formic acid as mobile phase. The MS data acquisition was accomplished by multiple reactions monitoring mode with positive electrospray ionization interface. The lower limit of quantification was 4 ng/mL; for inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 7%, and the accuracy was within 95.9-106.5%. The method is sensitive and simple, and was successfully applied to analysis of samples of clinical intoxication. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/20872940/Determination_of_pethidine_in_human_plasma_by_LC_MS/MS_ L2 - https://doi.org/10.1002/bmc.1524 DB - PRIME DP - Unbound Medicine ER -