Tags

Type your tag names separated by a space and hit enter

Co-expression of xylose reductase gene from Candida shehatae and endogenous xylitol dehydrogenase gene in Saccharomyces cerevisiae and the effect of metabolizing xylose to ethanol.
Prikl Biokhim Mikrobiol. 2010 Jul-Aug; 46(4):456-61.PB

Abstract

The inability of Saccharomyces cerevisiae to utilize xylose is attributed to its inability to convert xylose to xylulose. Low xylose reductase (XR) and xylitol dehydrogenase (XDH) activities in S. cerevisiae are regarded as the reason of blocking the pathway from xylose to xylulose. We had found that Candida shehatae could also be another source for XR gene except Pichia stipitis in the previous study. In this study, we tried to investigate if the expressed XR from C. shehatae could work with the over-expressed endogenous XDH together to achieve the same goal of converting xylose to ethanol in S. cerevisiae. The XR gene (XYL1) from C. shehatae and endogenous XDH gene (XYL2) were both cloned and over-expressed in host S. cerevisiae cell. The specific enzyme activities of XR and XDH were measured and the result of fermentation revealed that the new combination of two enzymes from different sources other than P. stipitis could also coordinate and work with each other and confer xylose utilization ability to S. cerevisiae.

Authors+Show Affiliations

College of Life Science, Capital Normal University, Beijing 100048, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20873171

Citation

Zhang, J, et al. "Co-expression of Xylose Reductase Gene From Candida Shehatae and Endogenous Xylitol Dehydrogenase Gene in Saccharomyces Cerevisiae and the Effect of Metabolizing Xylose to Ethanol." Prikladnaia Biokhimiia I Mikrobiologiia, vol. 46, no. 4, 2010, pp. 456-61.
Zhang J, Yang M, Tian S, et al. Co-expression of xylose reductase gene from Candida shehatae and endogenous xylitol dehydrogenase gene in Saccharomyces cerevisiae and the effect of metabolizing xylose to ethanol. Prikl Biokhim Mikrobiol. 2010;46(4):456-61.
Zhang, J., Yang, M., Tian, S., Zhang, Y., & Yang, X. (2010). Co-expression of xylose reductase gene from Candida shehatae and endogenous xylitol dehydrogenase gene in Saccharomyces cerevisiae and the effect of metabolizing xylose to ethanol. Prikladnaia Biokhimiia I Mikrobiologiia, 46(4), 456-61.
Zhang J, et al. Co-expression of Xylose Reductase Gene From Candida Shehatae and Endogenous Xylitol Dehydrogenase Gene in Saccharomyces Cerevisiae and the Effect of Metabolizing Xylose to Ethanol. Prikl Biokhim Mikrobiol. 2010 Jul-Aug;46(4):456-61. PubMed PMID: 20873171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-expression of xylose reductase gene from Candida shehatae and endogenous xylitol dehydrogenase gene in Saccharomyces cerevisiae and the effect of metabolizing xylose to ethanol. AU - Zhang,J, AU - Yang,M, AU - Tian,S, AU - Zhang,Y, AU - Yang,X, PY - 2010/9/29/entrez PY - 2010/9/29/pubmed PY - 2010/11/13/medline SP - 456 EP - 61 JF - Prikladnaia biokhimiia i mikrobiologiia JO - Prikl Biokhim Mikrobiol VL - 46 IS - 4 N2 - The inability of Saccharomyces cerevisiae to utilize xylose is attributed to its inability to convert xylose to xylulose. Low xylose reductase (XR) and xylitol dehydrogenase (XDH) activities in S. cerevisiae are regarded as the reason of blocking the pathway from xylose to xylulose. We had found that Candida shehatae could also be another source for XR gene except Pichia stipitis in the previous study. In this study, we tried to investigate if the expressed XR from C. shehatae could work with the over-expressed endogenous XDH together to achieve the same goal of converting xylose to ethanol in S. cerevisiae. The XR gene (XYL1) from C. shehatae and endogenous XDH gene (XYL2) were both cloned and over-expressed in host S. cerevisiae cell. The specific enzyme activities of XR and XDH were measured and the result of fermentation revealed that the new combination of two enzymes from different sources other than P. stipitis could also coordinate and work with each other and confer xylose utilization ability to S. cerevisiae. SN - 0555-1099 UR - https://www.unboundmedicine.com/medline/citation/20873171/Co_expression_of_xylose_reductase_gene_from_Candida_shehatae_and_endogenous_xylitol_dehydrogenase_gene_in_Saccharomyces_cerevisiae_and_the_effect_of_metabolizing_xylose_to_ethanol_ L2 - https://www.yeastgenome.org/reference/20873171 DB - PRIME DP - Unbound Medicine ER -