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Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli.
FEMS Microbiol Lett. 2010 Nov; 312(2):160-8.FM

Abstract

The transcription factor CsgD plays a key role in the control of biofilm formation in Escherichia coli by controlling the production of curli fimbriae and other biofilm components. In concert with its regulatory role, the promoter for the csgD operon is under the control of more than 10 regulatory factors, each monitoring a different condition or factor in stressful environments. Previously, we classified three factors (OmpR, RstA and IHF) as activators and two factors (CpxR and H-NS) as repressors, and found novel modes of their interplay. Here we describe an as yet uncharacterized regulator, MlrA, that has been suggested to participate in control of curli formation. Based on both in vivo and in vitro analyses, we identified MlrA as a positive factor of the csgD promoter by directly binding to its upstream region (-113 to -146) with a palindromic sequence of AAAATTGTACA(12N)TGTACAATTTT between the binding sites of two activators, IHF and OmpR. The possible interplay between three activators was analysed in detail.

Authors+Show Affiliations

Department of Frontier Bioscience, Hosei University, Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20874755

Citation

Ogasawara, Hiroshi, et al. "Regulatory Role of MlrA in Transcription Activation of csgD, the Master Regulator of Biofilm Formation in Escherichia Coli." FEMS Microbiology Letters, vol. 312, no. 2, 2010, pp. 160-8.
Ogasawara H, Yamamoto K, Ishihama A. Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli. FEMS Microbiol Lett. 2010;312(2):160-8.
Ogasawara, H., Yamamoto, K., & Ishihama, A. (2010). Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli. FEMS Microbiology Letters, 312(2), 160-8. https://doi.org/10.1111/j.1574-6968.2010.02112.x
Ogasawara H, Yamamoto K, Ishihama A. Regulatory Role of MlrA in Transcription Activation of csgD, the Master Regulator of Biofilm Formation in Escherichia Coli. FEMS Microbiol Lett. 2010;312(2):160-8. PubMed PMID: 20874755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli. AU - Ogasawara,Hiroshi, AU - Yamamoto,Kaneyoshi, AU - Ishihama,Akira, Y1 - 2010/09/27/ PY - 2010/9/30/entrez PY - 2010/9/30/pubmed PY - 2011/2/16/medline SP - 160 EP - 8 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 312 IS - 2 N2 - The transcription factor CsgD plays a key role in the control of biofilm formation in Escherichia coli by controlling the production of curli fimbriae and other biofilm components. In concert with its regulatory role, the promoter for the csgD operon is under the control of more than 10 regulatory factors, each monitoring a different condition or factor in stressful environments. Previously, we classified three factors (OmpR, RstA and IHF) as activators and two factors (CpxR and H-NS) as repressors, and found novel modes of their interplay. Here we describe an as yet uncharacterized regulator, MlrA, that has been suggested to participate in control of curli formation. Based on both in vivo and in vitro analyses, we identified MlrA as a positive factor of the csgD promoter by directly binding to its upstream region (-113 to -146) with a palindromic sequence of AAAATTGTACA(12N)TGTACAATTTT between the binding sites of two activators, IHF and OmpR. The possible interplay between three activators was analysed in detail. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/20874755/Regulatory_role_of_MlrA_in_transcription_activation_of_csgD_the_master_regulator_of_biofilm_formation_in_Escherichia_coli_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2010.02112.x DB - PRIME DP - Unbound Medicine ER -