Tags

Type your tag names separated by a space and hit enter

CELF and PTB proteins modulate the inclusion of the β-tropomyosin exon 6B during myogenic differentiation.
Exp Cell Res. 2011 Jan 01; 317(1):94-106.EC

Abstract

Exon 6B from the chicken β-tropomyosin pre-mRNA is alternatively spliced during myogenic differentiation. Exon 6B is excluded in mRNA from myoblasts and included in mRNA from myotubes. We investigated the regulation of exon 6B inclusion ex vivo in a quail myogenic cell line, which behaves as myoblasts in undifferentiated state and as myotubes after differentiation. We show that the β-tropomyosin exon 6B is a novel target of CUG-BP and ETR-3-like factor (CELF). Overexpression of CELF proteins in myoblasts activates splicing of exon 6B. Using a dominant-negative form of CELF4, we demonstrate that CELF proteins are involved in switching splicing from exon 6A towards exon 6B inclusion during myogenic differentiation. We also found that polypyrimidine tract binding protein (PTB) is required for splicing repression of exon 6B in myoblasts. CELF and PTB proteins exhibit antagonistic properties toward inclusion of exon 6B during myogenic differentiation. Our results suggest that a change in the protein level of CUGBP1 and PTB proteins, associated with a distinct pattern of PTB during the transition from myoblasts to myotubes is one of the parameters involved in regulating splicing of exon 6B during myogenesis.

Authors+Show Affiliations

Centre de Génétique Moléculaire, FRE 3144, CNRS, Gif sur Yvette, France, Université Paris XI, Orsay, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20875406

Citation

Sureau, Alain, et al. "CELF and PTB Proteins Modulate the Inclusion of the Β-tropomyosin Exon 6B During Myogenic Differentiation." Experimental Cell Research, vol. 317, no. 1, 2011, pp. 94-106.
Sureau A, Saulière J, Expert-Bezançon A, et al. CELF and PTB proteins modulate the inclusion of the β-tropomyosin exon 6B during myogenic differentiation. Exp Cell Res. 2011;317(1):94-106.
Sureau, A., Saulière, J., Expert-Bezançon, A., & Marie, J. (2011). CELF and PTB proteins modulate the inclusion of the β-tropomyosin exon 6B during myogenic differentiation. Experimental Cell Research, 317(1), 94-106. https://doi.org/10.1016/j.yexcr.2010.09.010
Sureau A, et al. CELF and PTB Proteins Modulate the Inclusion of the Β-tropomyosin Exon 6B During Myogenic Differentiation. Exp Cell Res. 2011 Jan 1;317(1):94-106. PubMed PMID: 20875406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CELF and PTB proteins modulate the inclusion of the β-tropomyosin exon 6B during myogenic differentiation. AU - Sureau,Alain, AU - Saulière,Jérôme, AU - Expert-Bezançon,Alain, AU - Marie,Joëlle, Y1 - 2010/09/25/ PY - 2010/07/07/received PY - 2010/09/14/revised PY - 2010/09/19/accepted PY - 2010/9/30/entrez PY - 2010/9/30/pubmed PY - 2010/12/16/medline SP - 94 EP - 106 JF - Experimental cell research JO - Exp Cell Res VL - 317 IS - 1 N2 - Exon 6B from the chicken β-tropomyosin pre-mRNA is alternatively spliced during myogenic differentiation. Exon 6B is excluded in mRNA from myoblasts and included in mRNA from myotubes. We investigated the regulation of exon 6B inclusion ex vivo in a quail myogenic cell line, which behaves as myoblasts in undifferentiated state and as myotubes after differentiation. We show that the β-tropomyosin exon 6B is a novel target of CUG-BP and ETR-3-like factor (CELF). Overexpression of CELF proteins in myoblasts activates splicing of exon 6B. Using a dominant-negative form of CELF4, we demonstrate that CELF proteins are involved in switching splicing from exon 6A towards exon 6B inclusion during myogenic differentiation. We also found that polypyrimidine tract binding protein (PTB) is required for splicing repression of exon 6B in myoblasts. CELF and PTB proteins exhibit antagonistic properties toward inclusion of exon 6B during myogenic differentiation. Our results suggest that a change in the protein level of CUGBP1 and PTB proteins, associated with a distinct pattern of PTB during the transition from myoblasts to myotubes is one of the parameters involved in regulating splicing of exon 6B during myogenesis. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/20875406/CELF_and_PTB_proteins_modulate_the_inclusion_of_the_β_tropomyosin_exon_6B_during_myogenic_differentiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(10)00446-5 DB - PRIME DP - Unbound Medicine ER -