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Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS).
Talanta. 2010 Oct 15; 82(5):1687-95.T

Abstract

Presented here is the quantitative analysis of green tea NIST standard reference materials (SRMs) via liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Three different NIST green tea standard reference materials (SRM 3254 Camellia sinesis Leaves, SRM 3255 C. sinesis Extract and SRM 3256 Green Tea-containing Oral Dosage Form) are characterized for the content of caffeine and a series of catechin species (gallic acid, catechin, epicatechin, epigallocatechin, epicatechin gallate and epigallocatechin gallate (EGCG)). The absolute limits of detection for caffeine and the catechin species were determined to be on the nanogram level. A reversed-phase chromatographic separation of the green tea reference materials was carried out on a commercial C(18) column using a gradient of water (containing 0.1% TFA) and 2:1 methanol:acetonitrile (containing 0.1%TFA) at 0.9mLmin(-1) and an analysis time of 50min. Quantification of caffeine and the catechin species was carried out using the standard addition and internal standard methods, with the latter providing appreciable improvements in precision and recovery.

Authors+Show Affiliations

Clemson University, Department of Chemistry, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634-1905, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20875564

Citation

Castro, Joaudimir, et al. "Determination of Catechins and Caffeine in Proposed Green Tea Standard Reference Materials By Liquid Chromatography-particle Beam/electron Ionization Mass Spectrometry (LC-PB/EIMS)." Talanta, vol. 82, no. 5, 2010, pp. 1687-95.
Castro J, Pregibon T, Chumanov K, et al. Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Talanta. 2010;82(5):1687-95.
Castro, J., Pregibon, T., Chumanov, K., & Marcus, R. K. (2010). Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Talanta, 82(5), 1687-95. https://doi.org/10.1016/j.talanta.2010.07.054
Castro J, et al. Determination of Catechins and Caffeine in Proposed Green Tea Standard Reference Materials By Liquid Chromatography-particle Beam/electron Ionization Mass Spectrometry (LC-PB/EIMS). Talanta. 2010 Oct 15;82(5):1687-95. PubMed PMID: 20875564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). AU - Castro,Joaudimir, AU - Pregibon,Tara, AU - Chumanov,Kristina, AU - Marcus,R Kenneth, Y1 - 2010/07/30/ PY - 2010/05/07/received PY - 2010/07/21/revised PY - 2010/07/22/accepted PY - 2010/9/30/entrez PY - 2010/9/30/pubmed PY - 2011/1/29/medline SP - 1687 EP - 95 JF - Talanta JO - Talanta VL - 82 IS - 5 N2 - Presented here is the quantitative analysis of green tea NIST standard reference materials (SRMs) via liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Three different NIST green tea standard reference materials (SRM 3254 Camellia sinesis Leaves, SRM 3255 C. sinesis Extract and SRM 3256 Green Tea-containing Oral Dosage Form) are characterized for the content of caffeine and a series of catechin species (gallic acid, catechin, epicatechin, epigallocatechin, epicatechin gallate and epigallocatechin gallate (EGCG)). The absolute limits of detection for caffeine and the catechin species were determined to be on the nanogram level. A reversed-phase chromatographic separation of the green tea reference materials was carried out on a commercial C(18) column using a gradient of water (containing 0.1% TFA) and 2:1 methanol:acetonitrile (containing 0.1%TFA) at 0.9mLmin(-1) and an analysis time of 50min. Quantification of caffeine and the catechin species was carried out using the standard addition and internal standard methods, with the latter providing appreciable improvements in precision and recovery. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20875564/Determination_of_catechins_and_caffeine_in_proposed_green_tea_standard_reference_materials_by_liquid_chromatography_particle_beam/electron_ionization_mass_spectrometry__LC_PB/EIMS__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00584-9 DB - PRIME DP - Unbound Medicine ER -