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Stacking and separation of aspartic acid enantiomers under discontinuous system by capillary electrophoresis with light-emitting diode-induced fluorescence detection.
Talanta. 2010 Oct 15; 82(5):1912-8.T

Abstract

We describe the stacking and separation of d- and l-aspartic acid (Asp) by capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF). In the presence of cyanide, d- and l-Asp were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to form fluorescent derivatives prior to CE-LEDIF. The separation of NDA-derivatized d- and l-Asp was accomplished using a discontinuous system - buffer vials contained a solution of 0.6% poly(ethylene oxide) (PEO), 150 mM sodium dodecyl sulfate (SDS), and 60mM hydroxypropyl-β-cyclodextrin (Hp-β-CD), while a capillary was filled with a solution of 150 mM SDS and 60mM Hp-β-CD. The role of PEO, Hp-β-CD, and SDS is to act as a concentrating media, as a chiral selector, and as a pseudostationary phase, respectively. This discontinuous system could be employed for the stacking of 600 nL of NDA-derivatized d- and l-Asp without the loss of chiral resolution. The stacking mechanism is mainly based on the difference in viscosity between sample zone and PEO as well as SDS sweeping. The limits of detection at signal-to-noise of 3 for d- and l-Asp were down to 2.4 and 2.5 × 10(-10)M, respectively. Compared to normal sample injection volume (25 nL), this stacking approach provided a 100- and 110-fold improvement in the sensitivity of d- and l-Asp, respectively. This method was further applied for determining d- and l-Asp in cerebrospinal fluid, soymilk, and beer.

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Authors+Show Affiliations

Lin KC
Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Taiwan.
Hsieh MM
No affiliation info available
Chang CW
No affiliation info available
Lin EP
No affiliation info available
Wu TH
No affiliation info available

MeSH

AnimalsAspartic AcidBeerChromatography, Micellar Electrokinetic CapillaryFluorescenceFluorescent DyesLimit of DetectionPolyethylene GlycolsSodium Dodecyl SulfateSoy MilkStereoisomerismalpha-Cyclodextrins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20875595

Citation

Lin, Kai-Cheng, et al. "Stacking and Separation of Aspartic Acid Enantiomers Under Discontinuous System By Capillary Electrophoresis With Light-emitting Diode-induced Fluorescence Detection." Talanta, vol. 82, no. 5, 2010, pp. 1912-8.
Lin KC, Hsieh MM, Chang CW, et al. Stacking and separation of aspartic acid enantiomers under discontinuous system by capillary electrophoresis with light-emitting diode-induced fluorescence detection. Talanta. 2010;82(5):1912-8.
Lin, K. C., Hsieh, M. M., Chang, C. W., Lin, E. P., & Wu, T. H. (2010). Stacking and separation of aspartic acid enantiomers under discontinuous system by capillary electrophoresis with light-emitting diode-induced fluorescence detection. Talanta, 82(5), 1912-8. https://doi.org/10.1016/j.talanta.2010.08.009
Lin KC, et al. Stacking and Separation of Aspartic Acid Enantiomers Under Discontinuous System By Capillary Electrophoresis With Light-emitting Diode-induced Fluorescence Detection. Talanta. 2010 Oct 15;82(5):1912-8. PubMed PMID: 20875595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stacking and separation of aspartic acid enantiomers under discontinuous system by capillary electrophoresis with light-emitting diode-induced fluorescence detection. AU - Lin,Kai-Cheng, AU - Hsieh,Ming-Mu, AU - Chang,Chia-Wei, AU - Lin,En-Ping, AU - Wu,Tsung-Han, Y1 - 2010/08/17/ PY - 2010/06/14/received PY - 2010/08/06/revised PY - 2010/08/09/accepted PY - 2010/9/30/entrez PY - 2010/9/30/pubmed PY - 2011/1/29/medline SP - 1912 EP - 8 JF - Talanta JO - Talanta VL - 82 IS - 5 N2 - We describe the stacking and separation of d- and l-aspartic acid (Asp) by capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF). In the presence of cyanide, d- and l-Asp were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to form fluorescent derivatives prior to CE-LEDIF. The separation of NDA-derivatized d- and l-Asp was accomplished using a discontinuous system - buffer vials contained a solution of 0.6% poly(ethylene oxide) (PEO), 150 mM sodium dodecyl sulfate (SDS), and 60mM hydroxypropyl-β-cyclodextrin (Hp-β-CD), while a capillary was filled with a solution of 150 mM SDS and 60mM Hp-β-CD. The role of PEO, Hp-β-CD, and SDS is to act as a concentrating media, as a chiral selector, and as a pseudostationary phase, respectively. This discontinuous system could be employed for the stacking of 600 nL of NDA-derivatized d- and l-Asp without the loss of chiral resolution. The stacking mechanism is mainly based on the difference in viscosity between sample zone and PEO as well as SDS sweeping. The limits of detection at signal-to-noise of 3 for d- and l-Asp were down to 2.4 and 2.5 × 10(-10)M, respectively. Compared to normal sample injection volume (25 nL), this stacking approach provided a 100- and 110-fold improvement in the sensitivity of d- and l-Asp, respectively. This method was further applied for determining d- and l-Asp in cerebrospinal fluid, soymilk, and beer. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20875595/Stacking_and_separation_of_aspartic_acid_enantiomers_under_discontinuous_system_by_capillary_electrophoresis_with_light_emitting_diode_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00626-0 DB - PRIME DP - Unbound Medicine ER -
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