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Core-shell nanostructures for ultrasensitive detection of α-thrombin.
Nanoscale. 2010 Dec; 2(12):2841-6.N

Abstract

We have synthesized a stable, sensitive and specific surface-enhanced Raman tag, and demonstrated its application in human α-thrombin detection. The tag consists of aptamer-modified core-shell nanoparticles with hydrophobic Au@Ag as core and silica as shell encapsulating Raman active molecules. By taking advantage of the Raman signal enhancement effect by metallic nanostructures, high stability and robustness of glass-coated core-shell nanostructures and the recognition capabilities of aptamers, we designed a sandwich detection for protein identification with high selectivity and sensitivity. In this way, we realized the ultrasensitive detection of α-thrombin. GDNs (glass-coated, dye-tagged nanoparticles), which were conjugated with oligonucleotides or antibodies, were extremely soluble in water, and had mechanical and chemical stability, easily controllable-size distribution and friendly biocompatibility. Specifically, the glass coating renders the particles amenable to use in many solvents without altering the Raman spectral response and makes agglomeration a nonfactor. All these merits open the door of the real applications in diagnostics or medical investigations in complex biofluids, such as human plasma and serum. Using the aptamer-modified GDNs as Raman tags, we successfully performed the detection of α-thrombin in human plasma. Furthermore, the overall method have been proved effective and selective, and may be implemented for multiplex target analysis simultaneously.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20877894

Citation

Chen, Xia, et al. "Core-shell Nanostructures for Ultrasensitive Detection of Α-thrombin." Nanoscale, vol. 2, no. 12, 2010, pp. 2841-6.
Chen X, Liu H, Zhou X, et al. Core-shell nanostructures for ultrasensitive detection of α-thrombin. Nanoscale. 2010;2(12):2841-6.
Chen, X., Liu, H., Zhou, X., & Hu, J. (2010). Core-shell nanostructures for ultrasensitive detection of α-thrombin. Nanoscale, 2(12), 2841-6. https://doi.org/10.1039/c0nr00318b
Chen X, et al. Core-shell Nanostructures for Ultrasensitive Detection of Α-thrombin. Nanoscale. 2010;2(12):2841-6. PubMed PMID: 20877894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Core-shell nanostructures for ultrasensitive detection of α-thrombin. AU - Chen,Xia, AU - Liu,Hongli, AU - Zhou,Xiaodong, AU - Hu,Jiming, Y1 - 2010/09/29/ PY - 2010/9/30/entrez PY - 2010/9/30/pubmed PY - 2011/3/22/medline SP - 2841 EP - 6 JF - Nanoscale JO - Nanoscale VL - 2 IS - 12 N2 - We have synthesized a stable, sensitive and specific surface-enhanced Raman tag, and demonstrated its application in human α-thrombin detection. The tag consists of aptamer-modified core-shell nanoparticles with hydrophobic Au@Ag as core and silica as shell encapsulating Raman active molecules. By taking advantage of the Raman signal enhancement effect by metallic nanostructures, high stability and robustness of glass-coated core-shell nanostructures and the recognition capabilities of aptamers, we designed a sandwich detection for protein identification with high selectivity and sensitivity. In this way, we realized the ultrasensitive detection of α-thrombin. GDNs (glass-coated, dye-tagged nanoparticles), which were conjugated with oligonucleotides or antibodies, were extremely soluble in water, and had mechanical and chemical stability, easily controllable-size distribution and friendly biocompatibility. Specifically, the glass coating renders the particles amenable to use in many solvents without altering the Raman spectral response and makes agglomeration a nonfactor. All these merits open the door of the real applications in diagnostics or medical investigations in complex biofluids, such as human plasma and serum. Using the aptamer-modified GDNs as Raman tags, we successfully performed the detection of α-thrombin in human plasma. Furthermore, the overall method have been proved effective and selective, and may be implemented for multiplex target analysis simultaneously. SN - 2040-3372 UR - https://www.unboundmedicine.com/medline/citation/20877894/Core_shell_nanostructures_for_ultrasensitive_detection_of_α_thrombin_ L2 - https://doi.org/10.1039/c0nr00318b DB - PRIME DP - Unbound Medicine ER -