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Satellite cell heterogeneity with respect to expression of MyoD, myogenin, Dlk1 and c-Met in human skeletal muscle: application to a cohort of power lifters and sedentary men.
Histochem Cell Biol. 2010 Oct; 134(4):371-85.HC

Abstract

Human satellite cells (SCs) are heterogeneous with respect to markers for their identification in the niche between the muscle fibre plasma membrane and its basal lamina. We have previously shown that, in biopsies from highly competitive power lifters, power lifters with long-term use of anabolic steroids and a population of healthy sedentary men, antibodies against the neuronal cell adhesion molecule (NCAM) and the paired box transcription factor Pax7 together label 94% of the SCs, NCAM alone labels 4% and Pax7 alone labels 1%. In the present study, we have further studied these biopsies with four markers related to SC activation and differentiation. Our study unequivocally shows that staining for MyoD and myogenin are present in nuclei of SCs and of myoblasts and myotubes in areas of muscle fibre regeneration. Staining for c-Met was observed in a proportion of Pax7+ SCs. However, widespread labelling of the sarcolemma precluded the quantification of c-Met+/Pax7+ SCs and the use of c-Met as a reliable SC marker. Pax7+ SCs labelled by anti-Delta like1 (Dlk1) were present in all samples but in variable proportions, whereas muscle progenitor cells related to repair were Dlk1⁻. Staining for Dlk1 was also observed in Pax7⁻ interstitial cells and in the cytoplasm of some small muscle fibres. Interestingly, the proportion of Dlk1+/Pax7+ SCs was significantly different between the groups of power lifters. Thus, our study confirms that human SCs show marked heterogeneity and this is discussed in terms of SC activation, myonuclei turnover, muscle fibre growth and muscle fibre damage and repair.

Authors+Show Affiliations

Section for Anatomy, Department of Integrative Medical Biology, Umeå University, 90187 Umeå, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20878332

Citation

Lindström, Mona, et al. "Satellite Cell Heterogeneity With Respect to Expression of MyoD, Myogenin, Dlk1 and c-Met in Human Skeletal Muscle: Application to a Cohort of Power Lifters and Sedentary Men." Histochemistry and Cell Biology, vol. 134, no. 4, 2010, pp. 371-85.
Lindström M, Pedrosa-Domellöf F, Thornell LE. Satellite cell heterogeneity with respect to expression of MyoD, myogenin, Dlk1 and c-Met in human skeletal muscle: application to a cohort of power lifters and sedentary men. Histochem Cell Biol. 2010;134(4):371-85.
Lindström, M., Pedrosa-Domellöf, F., & Thornell, L. E. (2010). Satellite cell heterogeneity with respect to expression of MyoD, myogenin, Dlk1 and c-Met in human skeletal muscle: application to a cohort of power lifters and sedentary men. Histochemistry and Cell Biology, 134(4), 371-85. https://doi.org/10.1007/s00418-010-0743-5
Lindström M, Pedrosa-Domellöf F, Thornell LE. Satellite Cell Heterogeneity With Respect to Expression of MyoD, Myogenin, Dlk1 and c-Met in Human Skeletal Muscle: Application to a Cohort of Power Lifters and Sedentary Men. Histochem Cell Biol. 2010;134(4):371-85. PubMed PMID: 20878332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Satellite cell heterogeneity with respect to expression of MyoD, myogenin, Dlk1 and c-Met in human skeletal muscle: application to a cohort of power lifters and sedentary men. AU - Lindström,Mona, AU - Pedrosa-Domellöf,Fatima, AU - Thornell,Lars-Eric, Y1 - 2010/09/28/ PY - 2010/09/08/accepted PY - 2010/9/30/entrez PY - 2010/9/30/pubmed PY - 2011/3/22/medline SP - 371 EP - 85 JF - Histochemistry and cell biology JO - Histochem Cell Biol VL - 134 IS - 4 N2 - Human satellite cells (SCs) are heterogeneous with respect to markers for their identification in the niche between the muscle fibre plasma membrane and its basal lamina. We have previously shown that, in biopsies from highly competitive power lifters, power lifters with long-term use of anabolic steroids and a population of healthy sedentary men, antibodies against the neuronal cell adhesion molecule (NCAM) and the paired box transcription factor Pax7 together label 94% of the SCs, NCAM alone labels 4% and Pax7 alone labels 1%. In the present study, we have further studied these biopsies with four markers related to SC activation and differentiation. Our study unequivocally shows that staining for MyoD and myogenin are present in nuclei of SCs and of myoblasts and myotubes in areas of muscle fibre regeneration. Staining for c-Met was observed in a proportion of Pax7+ SCs. However, widespread labelling of the sarcolemma precluded the quantification of c-Met+/Pax7+ SCs and the use of c-Met as a reliable SC marker. Pax7+ SCs labelled by anti-Delta like1 (Dlk1) were present in all samples but in variable proportions, whereas muscle progenitor cells related to repair were Dlk1⁻. Staining for Dlk1 was also observed in Pax7⁻ interstitial cells and in the cytoplasm of some small muscle fibres. Interestingly, the proportion of Dlk1+/Pax7+ SCs was significantly different between the groups of power lifters. Thus, our study confirms that human SCs show marked heterogeneity and this is discussed in terms of SC activation, myonuclei turnover, muscle fibre growth and muscle fibre damage and repair. SN - 1432-119X UR - https://www.unboundmedicine.com/medline/citation/20878332/Satellite_cell_heterogeneity_with_respect_to_expression_of_MyoD_myogenin_Dlk1_and_c_Met_in_human_skeletal_muscle:_application_to_a_cohort_of_power_lifters_and_sedentary_men_ L2 - https://dx.doi.org/10.1007/s00418-010-0743-5 DB - PRIME DP - Unbound Medicine ER -