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Resolvin D1 attenuates activation of sensory transient receptor potential channels leading to multiple anti-nociception.
Br J Pharmacol. 2010 Oct; 161(3):707-20.BJ

Abstract

BACKGROUND AND PURPOSE

Temperature-sensitive transient receptor potential ion channels (thermoTRPs) expressed in primary sensory neurons and skin keratinocytes play a crucial role as peripheral pain detectors. Many natural and synthetic ligands have been found to act on thermoTRPs, but little is known about endogenous compounds that inhibit these TRPs. Here, we asked whether resolvin D1 (RvD1), a naturally occurring anti-inflammatory and pro-resolving lipid molecule is able to affect the TRP channel activation.

EXPERIMENTAL APPROACH

We examined the effect of RvD1 on the six thermoTRPs using Ca(2+) imaging and whole cell electrophysiology experiments using the HEK cell heterologous expression system, cultured sensory neurons and HaCaT keratinocytes. We also checked changes in agonist-specific acute licking/flicking or flinching behaviours and TRP-related mechanical and thermal pain behaviours using Hargreaves, Randall-Selitto and von Frey assay systems with or without inflammation.

KEY RESULTS

RvD1 inhibited the activities of TRPA1, TRPV3 and TRPV4 at nanomolar and micromolar levels. Consistent attenuations in agonist-specific acute pain behaviours by immediate peripheral administration with RvD1 were also observed. Furthermore, local pretreatment with RvD1 significantly reversed mechanical and thermal hypersensitivity in inflamed tissues.

CONCLUSIONS AND IMPLICATIONS

RvD1 was a novel endogenous inhibitor for several sensory TRPs. The results of our behavioural studies suggest that RvD1 has an analgesic potential via these TRP-related mechanisms.

Authors+Show Affiliations

Korea University Graduate School of Medicine, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20880407

Citation

Bang, S, et al. "Resolvin D1 Attenuates Activation of Sensory Transient Receptor Potential Channels Leading to Multiple Anti-nociception." British Journal of Pharmacology, vol. 161, no. 3, 2010, pp. 707-20.
Bang S, Yoo S, Yang TJ, et al. Resolvin D1 attenuates activation of sensory transient receptor potential channels leading to multiple anti-nociception. Br J Pharmacol. 2010;161(3):707-20.
Bang, S., Yoo, S., Yang, T. J., Cho, H., Kim, Y. G., & Hwang, S. W. (2010). Resolvin D1 attenuates activation of sensory transient receptor potential channels leading to multiple anti-nociception. British Journal of Pharmacology, 161(3), 707-20. https://doi.org/10.1111/j.1476-5381.2010.00909.x
Bang S, et al. Resolvin D1 Attenuates Activation of Sensory Transient Receptor Potential Channels Leading to Multiple Anti-nociception. Br J Pharmacol. 2010;161(3):707-20. PubMed PMID: 20880407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resolvin D1 attenuates activation of sensory transient receptor potential channels leading to multiple anti-nociception. AU - Bang,S, AU - Yoo,S, AU - Yang,T J, AU - Cho,H, AU - Kim,Y G, AU - Hwang,S W, PY - 2010/10/1/entrez PY - 2010/10/1/pubmed PY - 2011/1/25/medline SP - 707 EP - 20 JF - British journal of pharmacology JO - Br J Pharmacol VL - 161 IS - 3 N2 - BACKGROUND AND PURPOSE: Temperature-sensitive transient receptor potential ion channels (thermoTRPs) expressed in primary sensory neurons and skin keratinocytes play a crucial role as peripheral pain detectors. Many natural and synthetic ligands have been found to act on thermoTRPs, but little is known about endogenous compounds that inhibit these TRPs. Here, we asked whether resolvin D1 (RvD1), a naturally occurring anti-inflammatory and pro-resolving lipid molecule is able to affect the TRP channel activation. EXPERIMENTAL APPROACH: We examined the effect of RvD1 on the six thermoTRPs using Ca(2+) imaging and whole cell electrophysiology experiments using the HEK cell heterologous expression system, cultured sensory neurons and HaCaT keratinocytes. We also checked changes in agonist-specific acute licking/flicking or flinching behaviours and TRP-related mechanical and thermal pain behaviours using Hargreaves, Randall-Selitto and von Frey assay systems with or without inflammation. KEY RESULTS: RvD1 inhibited the activities of TRPA1, TRPV3 and TRPV4 at nanomolar and micromolar levels. Consistent attenuations in agonist-specific acute pain behaviours by immediate peripheral administration with RvD1 were also observed. Furthermore, local pretreatment with RvD1 significantly reversed mechanical and thermal hypersensitivity in inflamed tissues. CONCLUSIONS AND IMPLICATIONS: RvD1 was a novel endogenous inhibitor for several sensory TRPs. The results of our behavioural studies suggest that RvD1 has an analgesic potential via these TRP-related mechanisms. SN - 1476-5381 UR - https://www.unboundmedicine.com/medline/citation/20880407/Resolvin_D1_attenuates_activation_of_sensory_transient_receptor_potential_channels_leading_to_multiple_anti_nociception_ L2 - https://doi.org/10.1111/j.1476-5381.2010.00909.x DB - PRIME DP - Unbound Medicine ER -