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GluN2B subunits of the NMDA receptor contribute to the AMPA receptor internalization during long-term depression in the lateral amygdala of juvenile rats.
Neuroscience. 2010 Dec 29; 171(4):1102-8.N

Abstract

The lateral nucleus of the amygdala (LA) is a critical structure involved in fear conditioning. We recently showed that regulated exocytosis and endocytosis of postsynaptic A-amino-3-hydroxy-5-methylisoxazole-4-propionate subtype of glutamate receptors (AMPARs) are involved in the expression of N-methyl-D-aspartate subtype glutamate receptors (NMDARs) dependent long-term potentiation (LTP) and long-term depression (LTD) in coronal slices of the LA. However, the molecular mechanisms of this effect remain unclear. In the present study, we investigated the role of distinct NMDAR subtypes in the endocytosis of AMPARs during LTD expression at the synapses of the thalamic inputs to the LA neurons. Here we show that the NMDARs antagonist DL-2-amino-5-phosphonovalerate (D-APV) blocked the induction of LTD and thus prevented endocytosis of surface AMPARs, indicating that NMDAR activation enhanced the internalization of AMPARs in LTD expression. Furthermore, the selective blocking of GluN2B-containing NMDARs completely abolished the NMDAR-induced AMPAR endocytosis, whereas preferential inhibition of GluN2A-containing NMDARs did not block the NMDAR-induced AMPAR endocytosis during LTD expression. These results suggest that there exist a preferred NMDAR subtype for AMPAR internalization and activation of GluN2B-containing NMDARs represent the predominate pathway triggered during the early stages of this NMDAR-induced endocytosis of AMPARs during LTD in the thalamic inputs to the LA of juvenile rats.

Authors+Show Affiliations

Department of Physiology, Shandong University, School of Medicine, Wenhuaxilu Road, Jinan, Shandong Province, 250012, PR China. shuyanyu@sdu.edu.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20884329

Citation

Yu, S Y., et al. "GluN2B Subunits of the NMDA Receptor Contribute to the AMPA Receptor Internalization During Long-term Depression in the Lateral Amygdala of Juvenile Rats." Neuroscience, vol. 171, no. 4, 2010, pp. 1102-8.
Yu SY, Wu DC, Zhan RZ. GluN2B subunits of the NMDA receptor contribute to the AMPA receptor internalization during long-term depression in the lateral amygdala of juvenile rats. Neuroscience. 2010;171(4):1102-8.
Yu, S. Y., Wu, D. C., & Zhan, R. Z. (2010). GluN2B subunits of the NMDA receptor contribute to the AMPA receptor internalization during long-term depression in the lateral amygdala of juvenile rats. Neuroscience, 171(4), 1102-8. https://doi.org/10.1016/j.neuroscience.2010.09.038
Yu SY, Wu DC, Zhan RZ. GluN2B Subunits of the NMDA Receptor Contribute to the AMPA Receptor Internalization During Long-term Depression in the Lateral Amygdala of Juvenile Rats. Neuroscience. 2010 Dec 29;171(4):1102-8. PubMed PMID: 20884329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GluN2B subunits of the NMDA receptor contribute to the AMPA receptor internalization during long-term depression in the lateral amygdala of juvenile rats. AU - Yu,S Y, AU - Wu,D C, AU - Zhan,R Z, Y1 - 2010/09/25/ PY - 2010/07/25/received PY - 2010/09/03/revised PY - 2010/09/22/accepted PY - 2010/10/2/entrez PY - 2010/10/5/pubmed PY - 2011/3/11/medline SP - 1102 EP - 8 JF - Neuroscience JO - Neuroscience VL - 171 IS - 4 N2 - The lateral nucleus of the amygdala (LA) is a critical structure involved in fear conditioning. We recently showed that regulated exocytosis and endocytosis of postsynaptic A-amino-3-hydroxy-5-methylisoxazole-4-propionate subtype of glutamate receptors (AMPARs) are involved in the expression of N-methyl-D-aspartate subtype glutamate receptors (NMDARs) dependent long-term potentiation (LTP) and long-term depression (LTD) in coronal slices of the LA. However, the molecular mechanisms of this effect remain unclear. In the present study, we investigated the role of distinct NMDAR subtypes in the endocytosis of AMPARs during LTD expression at the synapses of the thalamic inputs to the LA neurons. Here we show that the NMDARs antagonist DL-2-amino-5-phosphonovalerate (D-APV) blocked the induction of LTD and thus prevented endocytosis of surface AMPARs, indicating that NMDAR activation enhanced the internalization of AMPARs in LTD expression. Furthermore, the selective blocking of GluN2B-containing NMDARs completely abolished the NMDAR-induced AMPAR endocytosis, whereas preferential inhibition of GluN2A-containing NMDARs did not block the NMDAR-induced AMPAR endocytosis during LTD expression. These results suggest that there exist a preferred NMDAR subtype for AMPAR internalization and activation of GluN2B-containing NMDARs represent the predominate pathway triggered during the early stages of this NMDAR-induced endocytosis of AMPARs during LTD in the thalamic inputs to the LA of juvenile rats. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/20884329/GluN2B_subunits_of_the_NMDA_receptor_contribute_to_the_AMPA_receptor_internalization_during_long_term_depression_in_the_lateral_amygdala_of_juvenile_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(10)01286-8 DB - PRIME DP - Unbound Medicine ER -