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[Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats].

Abstract

OBJECTIVE

To explore the mechanism of electroacupuncture therapy on Parkinson's disease (PD).

METHODS

Fifty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. PD model was duplicated by microinjection of 6-Hydroxyl-Dopamine into right corpora striata, and by microinjection of normal saline in sham-operation group. Rats in normal group, sham-operation group and model group were not treated. In Fengfu-Taichong group, the rats were treated by electroacupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) on the basis of the PD model, and by electroacupuncture at "Fengfu" (GV 16), "Taichong" (LR 3), "Guanyuan" (CV 4) and "Zusanli" (ST 36) in Shuanggu Yitong group, once daily for 2 weeks. GDNF and Ret expression were detected by immunohistochemistry and western blotting, respectively.

RESULTS

The number of GDNF positive cells and the content of Ret receptor increased significantly in the two electroacupuncture groups compared with those in the other groups (all P < 0.01), and the expression of GDNF increased significantly in Shuanggu Yitong group compared with that in Fengfu-Taichong group (P < 0.01).

CONCLUSION

Electroacupuncture can not only increase the expression of GDNF, but also enhance its effect. "Shuanggu Yitong" method is better than simple acupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) in increasing expression of GDNF.

Authors+Show Affiliations

,

Hubei University of TCM, Wuhan, China.

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Source

MeSH

Animals
DNA-Binding Proteins
Disease Models, Animal
Electroacupuncture
Gene Expression
Glial Cell Line-Derived Neurotrophic Factor
Humans
Nuclear Proteins
Parkinson Disease
Random Allocation
Rats
Rats, Wistar

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

20886794

Citation

TY - JOUR T1 - [Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats]. AU - Wang,Yan-Chun, AU - Cheng,Yu-He, AU - Ma,Jun, AU - Gan,Shui-Yong, AU - Wang,Shu-Ju, AU - Zhou,Hua, AU - Du,Yan-Jun, AU - Yang,Min, AU - Shen,Feng, PY - 2010/10/5/entrez PY - 2010/10/5/pubmed PY - 2010/10/22/medline SP - 739 EP - 43 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 30 IS - 9 N2 - OBJECTIVE: To explore the mechanism of electroacupuncture therapy on Parkinson's disease (PD). METHODS: Fifty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. PD model was duplicated by microinjection of 6-Hydroxyl-Dopamine into right corpora striata, and by microinjection of normal saline in sham-operation group. Rats in normal group, sham-operation group and model group were not treated. In Fengfu-Taichong group, the rats were treated by electroacupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) on the basis of the PD model, and by electroacupuncture at "Fengfu" (GV 16), "Taichong" (LR 3), "Guanyuan" (CV 4) and "Zusanli" (ST 36) in Shuanggu Yitong group, once daily for 2 weeks. GDNF and Ret expression were detected by immunohistochemistry and western blotting, respectively. RESULTS: The number of GDNF positive cells and the content of Ret receptor increased significantly in the two electroacupuncture groups compared with those in the other groups (all P < 0.01), and the expression of GDNF increased significantly in Shuanggu Yitong group compared with that in Fengfu-Taichong group (P < 0.01). CONCLUSION: Electroacupuncture can not only increase the expression of GDNF, but also enhance its effect. "Shuanggu Yitong" method is better than simple acupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) in increasing expression of GDNF. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/20886794/ L2 - https://medlineplus.gov/parkinsonsdisease.html ER -