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[Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats].
Zhongguo Zhen Jiu 2010; 30(9):739-43ZZ

Abstract

OBJECTIVE

To explore the mechanism of electroacupuncture therapy on Parkinson's disease (PD).

METHODS

Fifty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. PD model was duplicated by microinjection of 6-Hydroxyl-Dopamine into right corpora striata, and by microinjection of normal saline in sham-operation group. Rats in normal group, sham-operation group and model group were not treated. In Fengfu-Taichong group, the rats were treated by electroacupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) on the basis of the PD model, and by electroacupuncture at "Fengfu" (GV 16), "Taichong" (LR 3), "Guanyuan" (CV 4) and "Zusanli" (ST 36) in Shuanggu Yitong group, once daily for 2 weeks. GDNF and Ret expression were detected by immunohistochemistry and western blotting, respectively.

RESULTS

The number of GDNF positive cells and the content of Ret receptor increased significantly in the two electroacupuncture groups compared with those in the other groups (all P < 0.01), and the expression of GDNF increased significantly in Shuanggu Yitong group compared with that in Fengfu-Taichong group (P < 0.01).

CONCLUSION

Electroacupuncture can not only increase the expression of GDNF, but also enhance its effect. "Shuanggu Yitong" method is better than simple acupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) in increasing expression of GDNF.

Authors+Show Affiliations

Hubei University of TCM, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

20886794

Citation

Wang, Yan-Chun, et al. "[Effects of Electroacupuncture On the Expression of GDNF and Ret in Parkinson's Disease Model Rats]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 30, no. 9, 2010, pp. 739-43.
Wang YC, Cheng YH, Ma J, et al. [Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats]. Zhongguo Zhen Jiu. 2010;30(9):739-43.
Wang, Y. C., Cheng, Y. H., Ma, J., Gan, S. Y., Wang, S. J., Zhou, H., ... Shen, F. (2010). [Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 30(9), pp. 739-43.
Wang YC, et al. [Effects of Electroacupuncture On the Expression of GDNF and Ret in Parkinson's Disease Model Rats]. Zhongguo Zhen Jiu. 2010;30(9):739-43. PubMed PMID: 20886794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats]. AU - Wang,Yan-Chun, AU - Cheng,Yu-He, AU - Ma,Jun, AU - Gan,Shui-Yong, AU - Wang,Shu-Ju, AU - Zhou,Hua, AU - Du,Yan-Jun, AU - Yang,Min, AU - Shen,Feng, PY - 2010/10/5/entrez PY - 2010/10/5/pubmed PY - 2010/10/22/medline SP - 739 EP - 43 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 30 IS - 9 N2 - OBJECTIVE: To explore the mechanism of electroacupuncture therapy on Parkinson's disease (PD). METHODS: Fifty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. PD model was duplicated by microinjection of 6-Hydroxyl-Dopamine into right corpora striata, and by microinjection of normal saline in sham-operation group. Rats in normal group, sham-operation group and model group were not treated. In Fengfu-Taichong group, the rats were treated by electroacupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) on the basis of the PD model, and by electroacupuncture at "Fengfu" (GV 16), "Taichong" (LR 3), "Guanyuan" (CV 4) and "Zusanli" (ST 36) in Shuanggu Yitong group, once daily for 2 weeks. GDNF and Ret expression were detected by immunohistochemistry and western blotting, respectively. RESULTS: The number of GDNF positive cells and the content of Ret receptor increased significantly in the two electroacupuncture groups compared with those in the other groups (all P < 0.01), and the expression of GDNF increased significantly in Shuanggu Yitong group compared with that in Fengfu-Taichong group (P < 0.01). CONCLUSION: Electroacupuncture can not only increase the expression of GDNF, but also enhance its effect. "Shuanggu Yitong" method is better than simple acupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) in increasing expression of GDNF. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/20886794/[Effects_of_electroacupuncture_on_the_expression_of_GDNF_and_Ret_in_Parkinson's_disease_model_rats]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -