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C-terminally truncated derivatives of Escherichia coli Hfq are proficient in riboregulation.
J Mol Biol. 2010 Nov 26; 404(2):173-82.JM

Abstract

The prokaryotic Sm-like protein Hfq plays an essential role in the stability and function of trans-encoded small regulatory RNAs in enterobacteria that function in posttranscriptional control by base-pairing with cognate target mRNAs. Hfq associates with both regulatory RNA and target RNA, and its interaction promotes annealing. So far, mutational and structural studies have established that Escherichia coli Hfq contains two separate RNA binding sites that are part of the conserved N-terminal portion of the protein. Moreover, it has been suggested that the nonconserved C-terminal extension of E. coli Hfq might constitute a third RNA interaction surface with specificity for mRNA. However, the role of the C-terminus has not been fully resolved but is clearly important for a complete understanding of Hfq function in posttranscriptional regulation and RNA decay. Here we examined the ability of E. coli Hfq derivatives, consisting of the conserved core and short C-terminal extensions, to support the regulation of rpoS expression and riboregulation by various well-characterized small regulatory RNAs. Our data show that, in all cases tested, the truncated proteins are fully capable of promoting posttranscriptional control, indicating that the C-terminal tail of E. coli Hfq plays a small role or no role in riboregulation.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20888338

Citation

Olsen, Anders Steno, et al. "C-terminally Truncated Derivatives of Escherichia Coli Hfq Are Proficient in Riboregulation." Journal of Molecular Biology, vol. 404, no. 2, 2010, pp. 173-82.
Olsen AS, Møller-Jensen J, Brennan RG, et al. C-terminally truncated derivatives of Escherichia coli Hfq are proficient in riboregulation. J Mol Biol. 2010;404(2):173-82.
Olsen, A. S., Møller-Jensen, J., Brennan, R. G., & Valentin-Hansen, P. (2010). C-terminally truncated derivatives of Escherichia coli Hfq are proficient in riboregulation. Journal of Molecular Biology, 404(2), 173-82. https://doi.org/10.1016/j.jmb.2010.09.038
Olsen AS, et al. C-terminally Truncated Derivatives of Escherichia Coli Hfq Are Proficient in Riboregulation. J Mol Biol. 2010 Nov 26;404(2):173-82. PubMed PMID: 20888338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - C-terminally truncated derivatives of Escherichia coli Hfq are proficient in riboregulation. AU - Olsen,Anders Steno, AU - Møller-Jensen,Jakob, AU - Brennan,Richard G, AU - Valentin-Hansen,Poul, Y1 - 2010/10/01/ PY - 2010/06/18/received PY - 2010/09/16/revised PY - 2010/09/18/accepted PY - 2010/10/5/entrez PY - 2010/10/5/pubmed PY - 2010/12/17/medline SP - 173 EP - 82 JF - Journal of molecular biology JO - J Mol Biol VL - 404 IS - 2 N2 - The prokaryotic Sm-like protein Hfq plays an essential role in the stability and function of trans-encoded small regulatory RNAs in enterobacteria that function in posttranscriptional control by base-pairing with cognate target mRNAs. Hfq associates with both regulatory RNA and target RNA, and its interaction promotes annealing. So far, mutational and structural studies have established that Escherichia coli Hfq contains two separate RNA binding sites that are part of the conserved N-terminal portion of the protein. Moreover, it has been suggested that the nonconserved C-terminal extension of E. coli Hfq might constitute a third RNA interaction surface with specificity for mRNA. However, the role of the C-terminus has not been fully resolved but is clearly important for a complete understanding of Hfq function in posttranscriptional regulation and RNA decay. Here we examined the ability of E. coli Hfq derivatives, consisting of the conserved core and short C-terminal extensions, to support the regulation of rpoS expression and riboregulation by various well-characterized small regulatory RNAs. Our data show that, in all cases tested, the truncated proteins are fully capable of promoting posttranscriptional control, indicating that the C-terminal tail of E. coli Hfq plays a small role or no role in riboregulation. SN - 1089-8638 UR - https://www.unboundmedicine.com/medline/citation/20888338/C_terminally_truncated_derivatives_of_Escherichia_coli_Hfq_are_proficient_in_riboregulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(10)01037-5 DB - PRIME DP - Unbound Medicine ER -