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LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries.
Vascul Pharmacol. 2010 Nov-Dec; 53(5-6):250-7.VP

Abstract

The purpose of our study was to examine if lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g.) modifies the vasomotor responses to Endothelin-1 (ET-1) and Sarafotoxin 6c (S6c) in rat coronary arteries. The arteries were studied directly or following organ culture for 24 h in absence and presence of 2.5EU/ml LPS. The contractile responses of coronary arteries were investigated by using the selective ETB receptor agonist S6c (1 pM-0.3 μM) and ET-1 (1 pM-0.3 μM). The functional studies demonstrated an augmented contractile response only to S6c in isolated rat coronary arteries after organ culture (with or without LPS). These contractile responses by S6c were blocked by the selective ETB receptor antagonist BQ788 in both vessel groups. The augmented contractile response to S6c was supported by immunohistochemistry, where a significant increase in fluorescence intensity for ETB receptors in smooth muscle cells was observed after organ culture. The presence of LPS in the culture medium significantly increased the sensitivity of endothelium-intact coronary artery to S6c as compared to endothelium-denuded segments. Our results showed a significant increase in both ETB receptor protein levels and S6c-induced maximal contraction in coronary arteries upon 24 h of organ culture, which was further sensitized by LPS.

Authors+Show Affiliations

Department of Periodontology, School of Dentistry, University of Copenhagen, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20888431

Citation

Ghorbani, Bahareh, et al. "LPS From Porphyromonas Gingivalis Increases the Sensitivity of Contractile Response Mediated By endothelin-B (ET(B)) Receptors in Cultured Endothelium-intact Rat Coronary Arteries." Vascular Pharmacology, vol. 53, no. 5-6, 2010, pp. 250-7.
Ghorbani B, Holmstrup P, Edvinsson L, et al. LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. Vascul Pharmacol. 2010;53(5-6):250-7.
Ghorbani, B., Holmstrup, P., Edvinsson, L., Kristiansen, K. A., & Sheykhzade, M. (2010). LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. Vascular Pharmacology, 53(5-6), 250-7. https://doi.org/10.1016/j.vph.2010.09.006
Ghorbani B, et al. LPS From Porphyromonas Gingivalis Increases the Sensitivity of Contractile Response Mediated By endothelin-B (ET(B)) Receptors in Cultured Endothelium-intact Rat Coronary Arteries. Vascul Pharmacol. 2010 Nov-Dec;53(5-6):250-7. PubMed PMID: 20888431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. AU - Ghorbani,Bahareh, AU - Holmstrup,Palle, AU - Edvinsson,Lars, AU - Kristiansen,Kim A, AU - Sheykhzade,Majid, Y1 - 2010/10/01/ PY - 2010/07/16/received PY - 2010/09/14/revised PY - 2010/09/24/accepted PY - 2010/10/5/entrez PY - 2010/10/5/pubmed PY - 2011/3/29/medline SP - 250 EP - 7 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 53 IS - 5-6 N2 - The purpose of our study was to examine if lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g.) modifies the vasomotor responses to Endothelin-1 (ET-1) and Sarafotoxin 6c (S6c) in rat coronary arteries. The arteries were studied directly or following organ culture for 24 h in absence and presence of 2.5EU/ml LPS. The contractile responses of coronary arteries were investigated by using the selective ETB receptor agonist S6c (1 pM-0.3 μM) and ET-1 (1 pM-0.3 μM). The functional studies demonstrated an augmented contractile response only to S6c in isolated rat coronary arteries after organ culture (with or without LPS). These contractile responses by S6c were blocked by the selective ETB receptor antagonist BQ788 in both vessel groups. The augmented contractile response to S6c was supported by immunohistochemistry, where a significant increase in fluorescence intensity for ETB receptors in smooth muscle cells was observed after organ culture. The presence of LPS in the culture medium significantly increased the sensitivity of endothelium-intact coronary artery to S6c as compared to endothelium-denuded segments. Our results showed a significant increase in both ETB receptor protein levels and S6c-induced maximal contraction in coronary arteries upon 24 h of organ culture, which was further sensitized by LPS. SN - 1879-3649 UR - https://www.unboundmedicine.com/medline/citation/20888431/LPS_from_Porphyromonas_gingivalis_increases_the_sensitivity_of_contractile_response_mediated_by_endothelin_B__ET_B___receptors_in_cultured_endothelium_intact_rat_coronary_arteries_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1537-1891(10)00134-5 DB - PRIME DP - Unbound Medicine ER -