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Maneb and paraquat-induced modulation of toxicant responsive genes in the rat liver: comparison with polymorphonuclear leukocytes.
Chem Biol Interact. 2010 Dec 05; 188(3):566-79.CB

Abstract

Experimental studies have shown that toxicant responsive genes, cytochrome P450s (CYPs) and glutathione S-transferases (GSTs) play a critical role in pesticide-induced toxicity. CYPs play pro-oxidant role and GSTs offer protection in maneb (MB) and paraquat (PQ)-induced brain and lung toxicities. The present study aimed to investigate the effect of repeated exposures of MB and/or PQ on lipid peroxidation (LPO), glutathione content (GSH) and toxicant responsive genes, i.e., CYP1A1, 1A2, 2E1, GSTA4-4, GSTA1-1 and GSTA3-3 in the liver and to correlate the same with polymorphonuclear leukocytes (PMNs). A significant augmentation in LPO and reduction in GSH content was observed in a time of exposure dependent manner in the liver and PMNs of MB and/or PQ treated animals. The expression and catalytic activity of CYP2E1 and GSTA4-4 were significantly increased following MB and/or PQ exposure both in the liver and PMNs. Although the expression of GSTA3-3 was increased, the expression of GSTA1-1 was unaltered after MB and/or PQ treatment in both the liver and PMNs. MB augmented the expression and catalytic activity of CYP1A1 in the liver, however, CYP1A2 was unaffected. PQ, on the other hand, significantly increased hepatic CYP1A2 expression and catalytic activity. MB and/or PQ did not produce any significant changes in CYP1A1 and CYP1A2 in PMNs. The results of the study thus demonstrate that MB and PQ differentially regulate hepatic CYP1A1 and CYP1A2 while LPO, GSH, CYP2E1, GSTA4-4 and GSTA3-3 are modulated in the similar fashions both in the liver and PMNs.

Authors+Show Affiliations

Indian Institute of Toxicology Research, Lucknow 226 001, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20888808

Citation

Ahmad, Israr, et al. "Maneb and Paraquat-induced Modulation of Toxicant Responsive Genes in the Rat Liver: Comparison With Polymorphonuclear Leukocytes." Chemico-biological Interactions, vol. 188, no. 3, 2010, pp. 566-79.
Ahmad I, Shukla S, Kumar A, et al. Maneb and paraquat-induced modulation of toxicant responsive genes in the rat liver: comparison with polymorphonuclear leukocytes. Chem Biol Interact. 2010;188(3):566-79.
Ahmad, I., Shukla, S., Kumar, A., Singh, B. K., Patel, D. K., Pandey, H. P., & Singh, C. (2010). Maneb and paraquat-induced modulation of toxicant responsive genes in the rat liver: comparison with polymorphonuclear leukocytes. Chemico-biological Interactions, 188(3), 566-79. https://doi.org/10.1016/j.cbi.2010.09.023
Ahmad I, et al. Maneb and Paraquat-induced Modulation of Toxicant Responsive Genes in the Rat Liver: Comparison With Polymorphonuclear Leukocytes. Chem Biol Interact. 2010 Dec 5;188(3):566-79. PubMed PMID: 20888808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maneb and paraquat-induced modulation of toxicant responsive genes in the rat liver: comparison with polymorphonuclear leukocytes. AU - Ahmad,Israr, AU - Shukla,Smriti, AU - Kumar,Ashutosh, AU - Singh,Brajesh Kumar, AU - Patel,Devendra Kumar, AU - Pandey,Haushila Prasad, AU - Singh,Chetna, Y1 - 2010/10/01/ PY - 2010/06/03/received PY - 2010/09/23/revised PY - 2010/09/23/accepted PY - 2010/10/5/entrez PY - 2010/10/5/pubmed PY - 2010/12/22/medline SP - 566 EP - 79 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 188 IS - 3 N2 - Experimental studies have shown that toxicant responsive genes, cytochrome P450s (CYPs) and glutathione S-transferases (GSTs) play a critical role in pesticide-induced toxicity. CYPs play pro-oxidant role and GSTs offer protection in maneb (MB) and paraquat (PQ)-induced brain and lung toxicities. The present study aimed to investigate the effect of repeated exposures of MB and/or PQ on lipid peroxidation (LPO), glutathione content (GSH) and toxicant responsive genes, i.e., CYP1A1, 1A2, 2E1, GSTA4-4, GSTA1-1 and GSTA3-3 in the liver and to correlate the same with polymorphonuclear leukocytes (PMNs). A significant augmentation in LPO and reduction in GSH content was observed in a time of exposure dependent manner in the liver and PMNs of MB and/or PQ treated animals. The expression and catalytic activity of CYP2E1 and GSTA4-4 were significantly increased following MB and/or PQ exposure both in the liver and PMNs. Although the expression of GSTA3-3 was increased, the expression of GSTA1-1 was unaltered after MB and/or PQ treatment in both the liver and PMNs. MB augmented the expression and catalytic activity of CYP1A1 in the liver, however, CYP1A2 was unaffected. PQ, on the other hand, significantly increased hepatic CYP1A2 expression and catalytic activity. MB and/or PQ did not produce any significant changes in CYP1A1 and CYP1A2 in PMNs. The results of the study thus demonstrate that MB and PQ differentially regulate hepatic CYP1A1 and CYP1A2 while LPO, GSH, CYP2E1, GSTA4-4 and GSTA3-3 are modulated in the similar fashions both in the liver and PMNs. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/20888808/Maneb_and_paraquat_induced_modulation_of_toxicant_responsive_genes_in_the_rat_liver:_comparison_with_polymorphonuclear_leukocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(10)00569-7 DB - PRIME DP - Unbound Medicine ER -