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Effects from filtration, capping agents, and presence/absence of food on the toxicity of silver nanoparticles to Daphnia magna.
Environ Toxicol Chem. 2010 Dec; 29(12):2742-50.ET

Abstract

Relatively little is known about the behavior and toxicity of nanoparticles in the environment. Objectives of work presented here include establishing the toxicity of a variety of silver nanoparticles (AgNPs) to Daphnia magna neonates, assessing the applicability of a commonly used bioassay for testing AgNPs, and determining the advantages and disadvantages of multiple characterization techniques for AgNPs in simple aquatic systems. Daphnia magna were exposed to a silver nitrate solution and AgNPs suspensions including commercially available AgNPs (uncoated and coated), and laboratory-synthesized AgNPs (coated with coffee or citrate). The nanoparticle suspensions were analyzed for silver concentration (microwave acid digestions), size (dynamic light scattering and electron microscopy), shape (electron microscopy), surface charge (zeta potentiometer), and chemical speciation (X-ray absorption spectroscopy, X-ray diffraction). Toxicities of filtered (100 nm) versus unfiltered suspensions were compared. Additionally, effects from addition of food were examined. Stock suspensions were prepared by adding AgNPs to moderately hard reconstituted water, which were then diluted and used straight or after filtration with 100-nm filters. All nanoparticle exposure suspensions, at every time interval, were digested via microwave digester and analyzed by inductively coupled argon plasma-optical emission spectroscopy or graphite furnace-atomic absorption spectroscopy. Dose-response curves were generated and median lethal concentration (LC50) values calculated. The LC50 values for the unfiltered particles were (in µg/L): 1.1 ± 0.1-AgNO(3) ; 1.0 ± 0.1-coffee coated; 1.1 ± 0.2-citrate coated; 16.7 ± 2.4 Sigma Aldrich Ag-nanoparticles (SA) uncoated; 31.5 ± 8.1 SA coated. LC50 values for the filtered particles were (in µg/L): 0.7 ± 0.1-AgNO(3) ; 1.4 ± 0.1-SA uncoated; 4.4 ± 1.4-SA coated. The LC50 resulting from the addition of food was 176.4 ± 25.5-SA coated. Recommendations presented in this study include AgNP handling methods, effects from sample preparation, and advantages/disadvantages of different nanoparticle characterization techniques.

Authors+Show Affiliations

US Environmental Protection Agency, Office of Research and Development, Cincinnati, Ohio, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20890913

Citation

Allen, H Joel, et al. "Effects From Filtration, Capping Agents, and Presence/absence of Food On the Toxicity of Silver Nanoparticles to Daphnia Magna." Environmental Toxicology and Chemistry, vol. 29, no. 12, 2010, pp. 2742-50.
Allen HJ, Impellitteri CA, Macke DA, et al. Effects from filtration, capping agents, and presence/absence of food on the toxicity of silver nanoparticles to Daphnia magna. Environ Toxicol Chem. 2010;29(12):2742-50.
Allen, H. J., Impellitteri, C. A., Macke, D. A., Heckman, J. L., Poynton, H. C., Lazorchak, J. M., Govindaswamy, S., Roose, D. L., & Nadagouda, M. N. (2010). Effects from filtration, capping agents, and presence/absence of food on the toxicity of silver nanoparticles to Daphnia magna. Environmental Toxicology and Chemistry, 29(12), 2742-50. https://doi.org/10.1002/etc.329
Allen HJ, et al. Effects From Filtration, Capping Agents, and Presence/absence of Food On the Toxicity of Silver Nanoparticles to Daphnia Magna. Environ Toxicol Chem. 2010;29(12):2742-50. PubMed PMID: 20890913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects from filtration, capping agents, and presence/absence of food on the toxicity of silver nanoparticles to Daphnia magna. AU - Allen,H Joel, AU - Impellitteri,Christopher A, AU - Macke,Dana A, AU - Heckman,J Lee, AU - Poynton,Helen C, AU - Lazorchak,James M, AU - Govindaswamy,Shekar, AU - Roose,Deborah L, AU - Nadagouda,Mallikarjuna N, Y1 - 2010/10/01/ PY - 2009/10/13/received PY - 2009/12/15/revised PY - 2010/06/20/accepted PY - 2010/10/5/entrez PY - 2010/10/5/pubmed PY - 2011/6/10/medline SP - 2742 EP - 50 JF - Environmental toxicology and chemistry JO - Environ Toxicol Chem VL - 29 IS - 12 N2 - Relatively little is known about the behavior and toxicity of nanoparticles in the environment. Objectives of work presented here include establishing the toxicity of a variety of silver nanoparticles (AgNPs) to Daphnia magna neonates, assessing the applicability of a commonly used bioassay for testing AgNPs, and determining the advantages and disadvantages of multiple characterization techniques for AgNPs in simple aquatic systems. Daphnia magna were exposed to a silver nitrate solution and AgNPs suspensions including commercially available AgNPs (uncoated and coated), and laboratory-synthesized AgNPs (coated with coffee or citrate). The nanoparticle suspensions were analyzed for silver concentration (microwave acid digestions), size (dynamic light scattering and electron microscopy), shape (electron microscopy), surface charge (zeta potentiometer), and chemical speciation (X-ray absorption spectroscopy, X-ray diffraction). Toxicities of filtered (100 nm) versus unfiltered suspensions were compared. Additionally, effects from addition of food were examined. Stock suspensions were prepared by adding AgNPs to moderately hard reconstituted water, which were then diluted and used straight or after filtration with 100-nm filters. All nanoparticle exposure suspensions, at every time interval, were digested via microwave digester and analyzed by inductively coupled argon plasma-optical emission spectroscopy or graphite furnace-atomic absorption spectroscopy. Dose-response curves were generated and median lethal concentration (LC50) values calculated. The LC50 values for the unfiltered particles were (in µg/L): 1.1 ± 0.1-AgNO(3) ; 1.0 ± 0.1-coffee coated; 1.1 ± 0.2-citrate coated; 16.7 ± 2.4 Sigma Aldrich Ag-nanoparticles (SA) uncoated; 31.5 ± 8.1 SA coated. LC50 values for the filtered particles were (in µg/L): 0.7 ± 0.1-AgNO(3) ; 1.4 ± 0.1-SA uncoated; 4.4 ± 1.4-SA coated. The LC50 resulting from the addition of food was 176.4 ± 25.5-SA coated. Recommendations presented in this study include AgNP handling methods, effects from sample preparation, and advantages/disadvantages of different nanoparticle characterization techniques. SN - 1552-8618 UR - https://www.unboundmedicine.com/medline/citation/20890913/Effects_from_filtration_capping_agents_and_presence/absence_of_food_on_the_toxicity_of_silver_nanoparticles_to_Daphnia_magna_ L2 - https://doi.org/10.1002/etc.329 DB - PRIME DP - Unbound Medicine ER -