Tags

Type your tag names separated by a space and hit enter

Effect of oxotremorine, physostigmine, and scopolamine on brain acetylcholine synthesis: a study using HPLC.
Neurochem Res. 1990 Nov; 15(11):1097-100.NR

Abstract

The synthesis rate of brain acetylcholine (ACh) was estimated in mice following i.v. administration of [3H]choline (Ch). The measurements were performed 1 min after the tracer injection, using the [3H]ACh/[3H]Ch specific radioactivity ratio as an index of ACh synthesis rate. Endogenous and labeled Ch and ACh were quantified using HPLC methodology. Oxotremorine and physostigmine (0.5 mg/kg, i.p.) increased the steady state concentration of brain ACh by + 130% and 84%, respectively and of Ch by + 60% (oxotremorine); they decreased ACh synthesis by 62 and 55%, respectively. By contrast, scopolamine (0.7 mg/kg, i.p.) decreased the cerebral content of Ch by - 26% and of ACh by - 23% without enhancing the synthesis of ACh. The results show the utility of HPLC methodology in the investigation of ACh turnover.

Authors+Show Affiliations

Laboratoire de Pharmacodynamie et de Physiologie Pharmaceutique, Faculté de Pharmacie, Dijon, France.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2089268

Citation

Bertrand, N, and A Beley. "Effect of Oxotremorine, Physostigmine, and Scopolamine On Brain Acetylcholine Synthesis: a Study Using HPLC." Neurochemical Research, vol. 15, no. 11, 1990, pp. 1097-100.
Bertrand N, Beley A. Effect of oxotremorine, physostigmine, and scopolamine on brain acetylcholine synthesis: a study using HPLC. Neurochem Res. 1990;15(11):1097-100.
Bertrand, N., & Beley, A. (1990). Effect of oxotremorine, physostigmine, and scopolamine on brain acetylcholine synthesis: a study using HPLC. Neurochemical Research, 15(11), 1097-100.
Bertrand N, Beley A. Effect of Oxotremorine, Physostigmine, and Scopolamine On Brain Acetylcholine Synthesis: a Study Using HPLC. Neurochem Res. 1990;15(11):1097-100. PubMed PMID: 2089268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of oxotremorine, physostigmine, and scopolamine on brain acetylcholine synthesis: a study using HPLC. AU - Bertrand,N, AU - Beley,A, PY - 1990/11/1/pubmed PY - 1990/11/1/medline PY - 1990/11/1/entrez SP - 1097 EP - 100 JF - Neurochemical research JO - Neurochem Res VL - 15 IS - 11 N2 - The synthesis rate of brain acetylcholine (ACh) was estimated in mice following i.v. administration of [3H]choline (Ch). The measurements were performed 1 min after the tracer injection, using the [3H]ACh/[3H]Ch specific radioactivity ratio as an index of ACh synthesis rate. Endogenous and labeled Ch and ACh were quantified using HPLC methodology. Oxotremorine and physostigmine (0.5 mg/kg, i.p.) increased the steady state concentration of brain ACh by + 130% and 84%, respectively and of Ch by + 60% (oxotremorine); they decreased ACh synthesis by 62 and 55%, respectively. By contrast, scopolamine (0.7 mg/kg, i.p.) decreased the cerebral content of Ch by - 26% and of ACh by - 23% without enhancing the synthesis of ACh. The results show the utility of HPLC methodology in the investigation of ACh turnover. SN - 0364-3190 UR - https://www.unboundmedicine.com/medline/citation/2089268/Effect_of_oxotremorine_physostigmine_and_scopolamine_on_brain_acetylcholine_synthesis:_a_study_using_HPLC_ DB - PRIME DP - Unbound Medicine ER -