Tags

Type your tag names separated by a space and hit enter

Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach.
J Agric Food Chem. 2010 Oct 27; 58(20):10812-6.JA

Abstract

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method based on isocratic elution has been developed and validated for the simultaneous quantitation of flavonols (myricetin, quercetin, kaempferol, and isorhamnetin), flavones (luteolin and apigenin), and phenolic acids (chlorogenic, caffeic, ellagic, and rosmarinic acids) as important constituents in fruits, vegetables, and medicinal plants. Analysis was achieved on a C18 column at ambient temperature. The wavelengths used for the detection of flavonols, flavones, and phenolic acids were 370, 350, and 325 nm, respectively. After acid hydrolysis, the flavonoid aglycones were quantified straightforwardly in 20 dry herbal samples. The plants with the highest flavonoids were Rosa damascena, Solidago virgaurea, Ginkgo biloba, and Camellia sinensis. The contents of flavonoids aglycons ranged from 0.54 to 11.10 mg/g, from 0.03 to 14.80 mg/g, from 0.19 to 2.76 mg/g, from 0.15 to 2.36 mg/g, from 0.27 to 2.05 mg/g, and from 0.42 to 1.82 mg/g for quercetin, kaempferol, isorhamnetin, luteolin, apigenin, and myricetin in dry plant samples, respectively.

Authors+Show Affiliations

Jundi Shapour Research Center, Phytochemistry Group, 87135-1187 Kashan, Iran.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20919719

Citation

Haghi, Ghasem, and Alireza Hatami. "Simultaneous Quantification of Flavonoids and Phenolic Acids in Plant Materials By a Newly Developed Isocratic High-performance Liquid Chromatography Approach." Journal of Agricultural and Food Chemistry, vol. 58, no. 20, 2010, pp. 10812-6.
Haghi G, Hatami A. Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. J Agric Food Chem. 2010;58(20):10812-6.
Haghi, G., & Hatami, A. (2010). Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. Journal of Agricultural and Food Chemistry, 58(20), 10812-6. https://doi.org/10.1021/jf102175x
Haghi G, Hatami A. Simultaneous Quantification of Flavonoids and Phenolic Acids in Plant Materials By a Newly Developed Isocratic High-performance Liquid Chromatography Approach. J Agric Food Chem. 2010 Oct 27;58(20):10812-6. PubMed PMID: 20919719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. AU - Haghi,Ghasem, AU - Hatami,Alireza, Y1 - 2010/10/04/ PY - 2010/10/6/entrez PY - 2010/10/6/pubmed PY - 2014/12/15/medline SP - 10812 EP - 6 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 20 N2 - A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method based on isocratic elution has been developed and validated for the simultaneous quantitation of flavonols (myricetin, quercetin, kaempferol, and isorhamnetin), flavones (luteolin and apigenin), and phenolic acids (chlorogenic, caffeic, ellagic, and rosmarinic acids) as important constituents in fruits, vegetables, and medicinal plants. Analysis was achieved on a C18 column at ambient temperature. The wavelengths used for the detection of flavonols, flavones, and phenolic acids were 370, 350, and 325 nm, respectively. After acid hydrolysis, the flavonoid aglycones were quantified straightforwardly in 20 dry herbal samples. The plants with the highest flavonoids were Rosa damascena, Solidago virgaurea, Ginkgo biloba, and Camellia sinensis. The contents of flavonoids aglycons ranged from 0.54 to 11.10 mg/g, from 0.03 to 14.80 mg/g, from 0.19 to 2.76 mg/g, from 0.15 to 2.36 mg/g, from 0.27 to 2.05 mg/g, and from 0.42 to 1.82 mg/g for quercetin, kaempferol, isorhamnetin, luteolin, apigenin, and myricetin in dry plant samples, respectively. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20919719/Simultaneous_quantification_of_flavonoids_and_phenolic_acids_in_plant_materials_by_a_newly_developed_isocratic_high_performance_liquid_chromatography_approach_ L2 - https://doi.org/10.1021/jf102175x DB - PRIME DP - Unbound Medicine ER -