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Tobacco smoke exposure and altered nasal responses to live attenuated influenza virus.

Abstract

BACKGROUND

Epidemiologic evidence links tobacco smoke and increased risk for influenza in humans, but the specific host defense pathways involved are unclear.

OBJECTIVE

We developed a model to examine influenza-induced innate immune responses in humans and test the hypothesis that exposure to cigarette smoke alters nasal inflammatory and antiviral responses to live attenuated influenza virus (LAIV).

METHODS

This was an observational cohort study comparing nasal mucosal responses to LAIV among young adult active smokers (n = 17), nonsmokers exposed to secondhand smoke (SHS; n = 20), and unexposed controls (n = 23). Virus RNA and inflammatory factors were measured in nasal lavage fluids (NLF) serially after LAIV inoculation. For key end points, peak and total (area under curve) responses were compared among groups.

RESULTS

Compared with controls, NLF interleukin-6 (IL-6) responses to LAIV (peak and total) were suppressed in smokers. Virus RNA in NLF cells was significantly increased in smokers, as were interferon-inducible protein 10:virus ratios. Responses in SHS-exposed subjects were generally intermediate between controls and smokers. We observed significant associations between urine cotinine and NLF IL-6 responses (negative correlation) or virus RNA in NLF cells (positive correlation) for all subjects combined.

CONCLUSIONS

Nasal inoculation with LAIV results in measurable inflammatory and antiviral responses in human volunteers, thus providing a model for investigating environmental effects on influenza infections in humans. Exposure to cigarette smoke was associated with suppression of specific nasal inflammatory and antiviral responses, as well as increased virus quantity, after nasal inoculation with LAIV. These data suggest mechanisms for increased susceptibility to influenza infection among persons exposed to tobacco smoke.

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  • Authors+Show Affiliations

    ,

    Department of Pediatrics, University of North Carolina–Chapel Hill, Chapel Hill, North Carolina 27599-7310, USA. terry_noah@med.unc.edu

    , , , ,

    Source

    Environmental health perspectives 119:1 2011 Jan pg 78-83

    MeSH

    Adolescent
    Adult
    Chemokine CXCL10
    Female
    Humans
    Immunity, Mucosal
    Influenza, Human
    Interferon-gamma
    Interleukin-6
    Male
    Nasal Mucosa
    Orthomyxoviridae
    Prospective Studies
    Respiratory Tract Infections
    Rhinitis
    Risk Factors
    Tobacco Smoke Pollution
    Young Adult

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, Non-P.H.S.

    Language

    eng

    PubMed ID

    20920950

    Citation

    Noah, Terry L., et al. "Tobacco Smoke Exposure and Altered Nasal Responses to Live Attenuated Influenza Virus." Environmental Health Perspectives, vol. 119, no. 1, 2011, pp. 78-83.
    Noah TL, Zhou H, Monaco J, et al. Tobacco smoke exposure and altered nasal responses to live attenuated influenza virus. Environ Health Perspect. 2011;119(1):78-83.
    Noah, T. L., Zhou, H., Monaco, J., Horvath, K., Herbst, M., & Jaspers, I. (2011). Tobacco smoke exposure and altered nasal responses to live attenuated influenza virus. Environmental Health Perspectives, 119(1), pp. 78-83. doi:10.1289/ehp.1002258.
    Noah TL, et al. Tobacco Smoke Exposure and Altered Nasal Responses to Live Attenuated Influenza Virus. Environ Health Perspect. 2011;119(1):78-83. PubMed PMID: 20920950.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Tobacco smoke exposure and altered nasal responses to live attenuated influenza virus. AU - Noah,Terry L, AU - Zhou,Haibo, AU - Monaco,Jane, AU - Horvath,Katie, AU - Herbst,Margaret, AU - Jaspers,Ilona, Y1 - 2010/10/04/ PY - 2010/04/06/received PY - 2010/10/04/accepted PY - 2010/10/6/entrez PY - 2010/10/6/pubmed PY - 2011/3/23/medline SP - 78 EP - 83 JF - Environmental health perspectives JO - Environ. Health Perspect. VL - 119 IS - 1 N2 - BACKGROUND: Epidemiologic evidence links tobacco smoke and increased risk for influenza in humans, but the specific host defense pathways involved are unclear. OBJECTIVE: We developed a model to examine influenza-induced innate immune responses in humans and test the hypothesis that exposure to cigarette smoke alters nasal inflammatory and antiviral responses to live attenuated influenza virus (LAIV). METHODS: This was an observational cohort study comparing nasal mucosal responses to LAIV among young adult active smokers (n = 17), nonsmokers exposed to secondhand smoke (SHS; n = 20), and unexposed controls (n = 23). Virus RNA and inflammatory factors were measured in nasal lavage fluids (NLF) serially after LAIV inoculation. For key end points, peak and total (area under curve) responses were compared among groups. RESULTS: Compared with controls, NLF interleukin-6 (IL-6) responses to LAIV (peak and total) were suppressed in smokers. Virus RNA in NLF cells was significantly increased in smokers, as were interferon-inducible protein 10:virus ratios. Responses in SHS-exposed subjects were generally intermediate between controls and smokers. We observed significant associations between urine cotinine and NLF IL-6 responses (negative correlation) or virus RNA in NLF cells (positive correlation) for all subjects combined. CONCLUSIONS: Nasal inoculation with LAIV results in measurable inflammatory and antiviral responses in human volunteers, thus providing a model for investigating environmental effects on influenza infections in humans. Exposure to cigarette smoke was associated with suppression of specific nasal inflammatory and antiviral responses, as well as increased virus quantity, after nasal inoculation with LAIV. These data suggest mechanisms for increased susceptibility to influenza infection among persons exposed to tobacco smoke. SN - 1552-9924 UR - https://www.unboundmedicine.com/medline/citation/20920950/Tobacco_smoke_exposure_and_altered_nasal_responses_to_live_attenuated_influenza_virus_ L2 - https://ehp.niehs.nih.gov/doi/full/10.1289/ehp.1002258?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -