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Apocynum venetum leaf extract protects rat cortical neurons from injury induced by oxygen and glucose deprivation in vitro.
Can J Physiol Pharmacol. 2010 Sep; 88(9):907-17.CJ

Abstract

This study aimed to investigate the protective effect of Apocynum venetum leaf extract (AVLE) on an in vitro model of ischemia-reperfusion induced by oxygen and glucose deprivation (OGD) and further explored the possible mechanisms underlying protection. Cell injury was assessed by morphological examination using phase-contrast microscopy and quantified by measuring the amount of lactate dehydrogenase (LDH) leakage; cell viability was measured by XTT reduction. Neuronal apoptosis was determined by flow cytometry, and electron microscopy was used to study morphological changes of neurons. Caspase-3, -8, and -9 activation and Bcl-2/Bax protein expression were determined by Western blot analysis. We report that treatment with AVLE (5 and 50 µg/mL) effectively reduced neuronal cell death and relieved cell injury induced by OGD. Moreover, AVLE decreased the percentage of apoptotic neurons, relieved neuronal morphological damage, suppressed overexpression of active caspase-3 and -8 and Bax, and inhibited the reduction of Bcl-2 expression. These findings indicate that AVLE protects against OGD-induced injury by inhibiting apoptosis in rat cortical neurons by down-regulating caspase-3 activation and modulating the Bcl-2/Bax ratio.

Authors+Show Affiliations

Laboratory of Neurology, Institute of Integrative Medicine, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20921977

Citation

Xiang, Jun, et al. "Apocynum Venetum Leaf Extract Protects Rat Cortical Neurons From Injury Induced By Oxygen and Glucose Deprivation in Vitro." Canadian Journal of Physiology and Pharmacology, vol. 88, no. 9, 2010, pp. 907-17.
Xiang J, Tang YP, Zhou ZY, et al. Apocynum venetum leaf extract protects rat cortical neurons from injury induced by oxygen and glucose deprivation in vitro. Can J Physiol Pharmacol. 2010;88(9):907-17.
Xiang, J., Tang, Y. P., Zhou, Z. Y., Wu, P., Wang, Z., Mori, M., & Cai, D. F. (2010). Apocynum venetum leaf extract protects rat cortical neurons from injury induced by oxygen and glucose deprivation in vitro. Canadian Journal of Physiology and Pharmacology, 88(9), 907-17. https://doi.org/10.1139/y10-069
Xiang J, et al. Apocynum Venetum Leaf Extract Protects Rat Cortical Neurons From Injury Induced By Oxygen and Glucose Deprivation in Vitro. Can J Physiol Pharmacol. 2010;88(9):907-17. PubMed PMID: 20921977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apocynum venetum leaf extract protects rat cortical neurons from injury induced by oxygen and glucose deprivation in vitro. AU - Xiang,Jun, AU - Tang,Yu-Ping, AU - Zhou,Zi-Yi, AU - Wu,Pin, AU - Wang,Zhong, AU - Mori,Masao, AU - Cai,Ding-Fang, PY - 2010/10/6/entrez PY - 2010/10/6/pubmed PY - 2011/1/25/medline SP - 907 EP - 17 JF - Canadian journal of physiology and pharmacology JO - Can J Physiol Pharmacol VL - 88 IS - 9 N2 - This study aimed to investigate the protective effect of Apocynum venetum leaf extract (AVLE) on an in vitro model of ischemia-reperfusion induced by oxygen and glucose deprivation (OGD) and further explored the possible mechanisms underlying protection. Cell injury was assessed by morphological examination using phase-contrast microscopy and quantified by measuring the amount of lactate dehydrogenase (LDH) leakage; cell viability was measured by XTT reduction. Neuronal apoptosis was determined by flow cytometry, and electron microscopy was used to study morphological changes of neurons. Caspase-3, -8, and -9 activation and Bcl-2/Bax protein expression were determined by Western blot analysis. We report that treatment with AVLE (5 and 50 µg/mL) effectively reduced neuronal cell death and relieved cell injury induced by OGD. Moreover, AVLE decreased the percentage of apoptotic neurons, relieved neuronal morphological damage, suppressed overexpression of active caspase-3 and -8 and Bax, and inhibited the reduction of Bcl-2 expression. These findings indicate that AVLE protects against OGD-induced injury by inhibiting apoptosis in rat cortical neurons by down-regulating caspase-3 activation and modulating the Bcl-2/Bax ratio. SN - 1205-7541 UR - https://www.unboundmedicine.com/medline/citation/20921977/Apocynum_venetum_leaf_extract_protects_rat_cortical_neurons_from_injury_induced_by_oxygen_and_glucose_deprivation_in_vitro_ L2 - https://cdnsciencepub.com/doi/10.1139/y10-069?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -